156 research outputs found
A multiplex real-time PCR method for detection of GSTM1 and GSTT1 copy numbers.
No full textObjectives: Deletion polymorphisms of Glutathione-S-transferase (GST) M I and T I are considered risk factors for various diseases. However, most previous studies only distinguished "null" and "non-null" genotypes. Our aim was to develop a reliable. high-throughput GSTM1/T1 genotyping method able to determine allele copy numbers. Design and methods: We developed a multiplex real time PCR method to distinguish between heterozygous (1/0) and homozygous (1/1) GSTM1 and GSTT1 genotypes. The principle of relative quantification was applied and an expectation-maximisation (EM) algorithm was developed to assign one of 3 possible genotypes: 1/1, 1/0 or 0/0 for each of the two genes. Results: 1320 Caucasians were genotyped using the newly developed method. The observed genotype distributions did not deviate from the expected and were in Hardly-Weinberg equilibrium. GSTM1 duplication was detected in one sample. Conclusion: This new semiquantitative genotyping method is a sensitive and promising tool for large-scale molecular epidemiological and clinical studies
Genetic polymorphisms of myeloperoxidase (MPO), epoxide hydrolase 1 (EPHX1) and NAD(P)H dehydrogenase 1 (NQO1) as risk factors of early onset lung caner
No full textCase-control study of genetic susceptibility in early onset lung cancer: Investigation of Matrix Metalloproteinase-1 (MMP1)
No full textGlutathione-S-transferase T1, M1 and P1 polymorphisms as risk factors for early onset lung cancer
No full textTo the question about the determinations of the terrorism in the works of the foreign jurisprudents
No full textThe Article is dedicated to study of the problem, undoubtedly actual and significant, which will promote the production of the ways of the fight with International Terrorism. The Author addresses to analysis one of a such aspect of the problem - a question about the base determination of the notion «terrorism» on foundation of the study of the multiple scientific works of the foreign jurisprudents, that has allowed to show the differences in the determinations of the Terrorism nowadays
N. S. Gumilev as a translator and an editor of “Robert Sothey. Ballads” of the publishing house “Vsemirnaya literature”
No full textN. Gumilev. The purpose of the publishing house was to produce collections of translations of the most famous literary works and to get Russian readers to acquaint with the world literature. Gumilev’s “Ballads” of Southey has pursued the same goal but with the characteristic of the Russian tradition of this poet’s reception. For the English public, Southey was mainly known as an author of historical epic poems, but in Russian literature he was more of a ballad master. This perception has made a lodgement thanks to the first and major translator of his poetry, V. A. Zhukovskiy whose favorite genre was a ballad. In the introduction to the collection of poems Gumilev has drawn attention to the importance of Zhukovskiy for the tradition of Russian Southey and has dedicated the whole book to this idea. Six ballads, the third part of all poems in the book are connected with Zhukovskiy: four of them were translated by him as individual poems and two more were included in a short hexametric novel “Two tales and one more” (“Mary, the maid of the Inn” and “Jaspar”). M. Gor’kiy didn’t like the idea of including Zhukovskiy’s translations in the book, but for Gumilev it was the question of high importance and he has struggled for it. His choice of the only ballad for his own translation also prove this concept: he has chosen “The Surgeon’s Warning”, Southey’s self-parody for one of his most noted poem in Russia thanks to Zhukovskiy’s translation
A revision of Heterocotyle (Monogenea: Monocotylidae) with a description of Heterocotyle capricornensis n. sp. from Himantura fai (Dasyatididae) from Heron Island, Great Barrier Reef, Australia
No full textHeterocotyle capricornensis n. sp. is described from the gills of Himantura fai collected from Heron Island, Great Barrier Reef, Australia. It is distinguished from other species in the genus by the morphology of the sclerotised male copulatory organ which is curved and tapered distally, the ovary which loops once before entering the oviduct, and the testis which has 3 posteriorly-directed lobes. The oncomiracidium of this species is also described. The homology between glands found in the oncomiracidium and the adult is discussed. Additional data based on the examination of type material and, in some cases, new material of previously described species are included. The genus is revised and a key to species is provided. Characters which unite the members of the genus could not be confirmed for Heterocotyle elliptica Pillai and Pillai, 1976 and H. robusta (Johnston and Tiegs, 1922) Price, 1938 and therefore we consider them species inquirendae. New host and locality records are presented for H. chinensis Timofeeva, 1983 and a new host record is presented for H. granulatae Young, 1967. Host-specificity and phylogenetic relationships in Heterocotyle are discussed
CYP450 polymorphisms as risk factors for early-onset lung cancer: Gender-specific differences.
No full textCytochrome P450 (CYP) enzymes, involved in metabolism of tobacco carcinogens, are also involved in estrogen metabolism and many are regulated by estrogens. These genes may thus be of relevance to gender-specific differences in lung cancer risk, particularly in early-onset lung cancer, where a high proportion of women is observed. We conducted a case-control study to investigate genetic polymorphisms in cytochromes that might modify the risk of developing early-onset lung cancer. In total, 638 Caucasian patients under the age of 51 with primary lung cancer and 1300 cancer-free control individuals, matched by age and sex, were included in this analysis. Thirteen polymorphisms in the CYP1A1, CYP1B1, CYP2A13, CYP3A4 and CYP3A5 genes were analyzed. No significant association was found for any of the analyzed polymorphisms and lung cancer risk overall. However, among women, a significantly increased risk of early-onset lung cancer was observed for carriers of the minor allele of CYP1B1 SNP rs1056836 [odds ratio (OR) 1.97; 95% confidence interval (CI) 1.32-2.94; P < 0.001]. Also, a non-significant increase in lung cancer risk was observed in the group of women carriers of the minor allele of CYP2A13 SNP rs1709084 (OR 1.64; 95% CI 1.00-2.70; P = 0.05). The effect of these two polymorphisms was shown to be modified by smoking. Haplotype analysis was performed for CYP1B1 and CYP2A13. No differences between cases and controls were observed for both genes (P = 0.63 and P = 0.42 for CYP1B1 and CYP2A13, respectively). Our results suggest that the CYP1B1 and the CYP2A13 genotypes may contribute to individual susceptibility to early-onset lung cancer in women
Synthesis and characterization of ZnBTC-based MOFs: effect of solvents and salt
No full textIn this work, we studied the optimization of synthetic approaches to creating structurally modified metal-organic frameworks under various synthesis conditions. We investigated the influence of the various solvents and zinc salts on the structural characteristics of the metal-organic framework based on benzene-1,3,5-tricarboxylic acid (H3BTC). The results indicate that the variation of the types of both solvent and salt is a parameter affecting the crystallinity, phase purity, and morphology of the metal-organic framework. This was confirmed by comprehensive structural characterization (SEM, EDX, PXRD)
Identification of lung cancer histology-specific variants applying Bayesian framework variant prioritization approaches within the TRICL and ILCCO consortia
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