1,647 research outputs found
Abscisic acid induces CBF gene transcription and subsequent induction of cold-regulated genes via the CRT promoter element
No full textMany cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA) as well as by cold. This has been thought to occur via two separate signaling pathways, with ABA acting via ABA-responsive promoter elements and low temperature activating the C-repeat element (CRT; dehydration-responsive) promoter element via CBF (DREB1) transcription factors. We show here that ABA is also capable of activating the CRT promoter element. Although the more recently discovered ABA-inducible CBF4 transcription factor might have accounted for this, we show here that CBF1-3 transcript levels also increase in response to elevated ABA levels. This increase in CBF1-3 transcript levels appears to be at least in part due to increased activity of the CBF promoters in response to ABA. A total of 125 bp of the CBF2 promoter, which has previously been shown to be sufficient for cold-, mechanical-, and cycloheximide-induced expression, was also sufficient for ABA-induced expression. However, the ABA-responsive promoter element-like motif within this region is not needed for ABA-induced expression. An observed increase in CBF protein levels after ABA treatment, together with previous data showing that increased CBF levels are sufficient for cold-regulated gene induction, suggests that ABA-induced increases in CBF1-3 transcript levels do have the potential to activate the CRT. Our data indicate therefore that activation of the CRT may also occur via a novel ABA-inducible signaling pathway using the normally cold-inducible CBFs
The ?-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana
No full textHeterotrimeric G-proteins have been implicated in having a role in many plant signalling pathways. To understand further the role of G-proteins, a preliminary experiment was performed to assess the impact of the G? subunit loss-of-function mutation gpa1-1 on the Arabidopsis transcriptome. The analysis indicated that the G? subunit may play a role in response to jasmonic acid (JA). Consistent with this, G? mutants showed a reduced response to JA in inhibition of chlorophyll accumulation and root growth, whilst G? gain-of-function plants overexpressing G? showed the opposite phenotype. The levels of JA and related compounds were unaffected in the gpa1-1 mutant, as was autoregulation of the Allene Oxide Synthase (AOS) gene that encodes a key enzyme for JA biosynthesis. In contrast, further analyses using G? loss- and gain-of-function Arabidopsis lines indicated that G? positively modulates the expression of the Vegetative Storage Protein (VSP) gene. This indicates that the G? subunit regulates a subset of JA-regulated genes defining a branch point in this signalling pathway in Arabidopsis. Further analysis of the impact of G? loss of function upon the JA-regulated transcriptome using Arabidopsis full genome arrays indicated that up to 29% of genes that are >2-fold regulated by JA in the wild type are misregulated in the G? mutant. This supports the observation that a significant proportion of, but not all, JA-regulated gene expression is mediated by G
Textbooks designed for students’ learning in the digital age
No full textKnight, BA ORCiD: 0000-0001-6627-378XIn this paper the author challenges the thoughts, practices and assumptions related to the creation, acquisition and use of textbooks and digital learning resources in the digital age. Firstly, the discussion centres on student characteristics in the culture of the knowledge society, and then examines the efficacy of
textbooks. Sophisticated Individualised Data Delivery Systems (SIDDS) (Knight 2015) are then introduced as the new “textbooks” to utilise the benefits of digital learning resources. The chapter will describe principles underpinning a learner-centred design framework as aspects to consider when designing learning experiences for diverse learners in textbooks incorporating new media formats (Fasso, Knight & Knight 2013)
Studies in Figured Tours of Knight in Two and Higher Dimensions
No full textTour of knight is over a millennium year old puzzle but ‘Figured tour’ of knight is a recent field of research. T. R. Dawson, an English chess problemist and the father of Fairy Chess, coined the term in 1940s. The name figured tour is appropriate for any numbered tour in which certain arithmetically related numbers are arranged in a geometrical pattern. Figured tours have been only looked into two-dimensional boards, mostly on 8x8 board. The author has constructed knight tour with square numbers in fiveleaper {3, 4} + {0, 5} path and various other figured tours on 6x6 board and extended it in three and four dimensional space. Construction of figured tours is a mathematical recreation and can also be used in pedagogy of higher mathematics
Potential use of Dicyphus hesperus Knight (Heteroptera: Miridae) for biological control of pests of greenhouse tomatoes
No full textThe potential of Dicyphus hesperus Knight as a predator of greenhouse whitefly, Trialeurodes vaporariorum (Westwood), and two-spotted spider mites, Tetranychus urticae Koch, was assessed in the laboratory and in a tomato greenhouse. D. hesperus adults fed readily upon both pest species in the laboratory, and nymphs completed development from egg to adult on either whitefly or mites. Whitefly, however, were a superior food for growth and development of D. hesperus compared to mites. Development time was shorter and resulting adult body size was larger for nymphs reared on whitefly compared to those reared on mites. In a greenhouse release, adults oriented to and oviposited on whitefly-infested sentinel plants but did not orient to mite-infested sentinel plants. D. hesperus adults oviposited on greenhouse tomatoes and their progeny completed development in a greenhouse in which both whitefly and mites were present. The results are discussed as they relate to the use of D. hesperus for biological control of pests of greenhouse vegetables.Peer reviewedFinal article publishedDicyphus hesperusgreenhouse vegetablestomatoomnivoresgeneralist predatorsHeteropterazoophytophagousTetranychus urticaeTrialeurodes vaporarioru
The regulation and role of oxidative signal-inducible 1 protein kinase in Arabidopsis thaliana
No full textWord processed copy.Includes bibliographical references (leaves 209-232).This study attempted to further characterise OXI1 protein kinase. Confocal microscopy and subcellular fractionation studies revealed a cytosolic localisation pattern for OXI1. Employment of a bioinformatics approach confirmed the induction of OXI1 gene expression in response to a range of AOS generating stimuli. However, the transcriptional increase of OXI1 in response to salinity and heat appears to be of no biological significance since the oxi1 mutant did not display altered tolerance to these two stresses in comparison to wild type
Battle of the Blockbusters: Joss Whedon as Public Pedagogue
No full textThis article discusses the concept of public pedagogy and the reasons for considering it relevant to the work of the writer/ director/ producer Joss Whedon, creator of numberous TV programmes, such as Buffy the Vampire Slayer, Angel and Firefly, and Films Serenity, Marvel's The Avengers and The Age of Ultron. It analyzes Marvel’s The Avengers (Whedon, 2012) and Christopher Nolan’s (2012b) The Dark Knight Rises as competing public pedagogies.It suggests that popular films can be seen as important educational projects; filmmakers have tremendous resources at their disposal and their creations have a global reach that cannot be matched by individual teachers or national education systems. Whedon can be seen as a radical educator; he enables his audiences to experience ways of looking at the world that challenge aspects of neo-liberal hegemony, and also encourages them to become critical thinkers who have to reflect on their own feelings and perspectives and resist simplistic perspectives on morality and the difficult political choices facing global society
Economic Growth in China: Productivity and Policy. Editorial Introduction.
No full texthttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000297470100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701EconomicsSocial Sciences, Mathematical MethodsStatistics & ProbabilitySCI(E)SSCI0EDITORIAL MATERIAL6,SI719-7217
The Oxford handbook of Neo-Latin /
No full textIncludes bibliographical references and index.Classical Latin, Medieval Latin, Neo-Latin / Keith Sidwell -- Neo-Latin's interplay with other languages / Demmy Verbeke -- Lyric poetry / Victoria Moul -- Narrative poetry / Florian Schaffenrath -- Epigram and occasional poetry / David Money -- Comedy / Stefan Tilg -- Tragedy / Gary R. Grund -- Oratory / Marc Van der Poel -- Political advice / Erik De Bom -- Historiography / Patrick Baker -- Letters / Jan Papy -- Fiction / Mark T. Riley -- Satire / Ingrid A.R. De Smet -- School / Robert Black -- University / Sarah Knight -- Philosophy / Guido Giglioni -- Science and medicine / Brian W. Ogilvie -- Contacts with the Arab world / Dag Nikolaus Hasse -- Biblical humanism / Andrew Taylor -- Catholicism / Jason Harris -- Protestantism / Irena Bakus -- Political action / Marc Laureys -- Gender / Diana Robin -- Social status / Françoise Waquet -- Italy /David Marsh -- France / Paul White -- The British isles / Estelle Haan -- The German-speaking countries / Robert Seidel -- Iberian peninsula / Alejandro Coroleu and Catarina Fouto -- The low countries / Dirk Sacré -- Scandinavia / Annika Ström and Peter Zeeberg -- East-central Europe / Cristina Neagu -- Colonial Spanish America and Brazil / Andrew Laird -- North America / Jean-François Cottier, Haijo Westra, and John Gallucci -- Asia / Noël Golvers
Low‐temperature perception leading to gene expression and cold tolerance in higher plants
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