117,521 research outputs found
«Un tirocinio aspro e ininterrotto». Architettura e vita di Adriano Marabini
No full textQuesto saggio è dedicato alla vita e alle opere dell’architetto Adriano Marabini. Dagli anni di formazione presso la Reale Accademia di Belle Arti di Bologna, passando per le prime esperienze professionali durante il Ventennio, fino all’intensa attività progettuale ed edilizia negli anni della ricostruzione, la lunga carriera di Marabini si intreccia con la sua vita personale, creando una complessa rete di relazioni che intercettano molti dei grandi temi dell’architettura italiana del Novecento. Tra questi, la costruzione di memoriali per la Grande Guerra e le architetture celebrative per il regime fascista, tra cui spicca la realizzazione della Casa del Fascio di Imola e alcuni progetti per servizi quali colonie montane e elioterapiche; i piani di ricostruzione urbana avviati nel secondo Dopoguerra, per i comuni di Camposanto (MO) e Solarolo (RA); i progetti di edilizia residenziale, dalle residenze private all’edilizia popolare del Villaggio “La Barca” di Bologna; l’architettura sacra postconciliare legata alla figura del cardinale Giacomo Lercaro; infine, la progettazione architettonica nell’Italia del boom, che include spazi per lo spettacolo, ovvero progetti e restauri di teatri e cinema, e spazi per il lavoro, quali stabilimenti industriali e uffici bancari. Se il baricentro geografico delle opere di Marabini ruota tra le città di Imola e Bologna, con realizzazioni che abbracciano anche la Romagna e le Marche, la sua esperienza tuttavia supera la dimensione locale e affronta tutte le grandi tappe del secolo breve. Ogni momento storico lascia infatti una traccia nella vita di Marabini: l’esperienza militare durante la Grande Guerra, l’affermarsi come professionista durante il regime fascista, il ruolo nei delicati anni della ricostruzione nel secondo Dopoguerra. Attraverso il ricco materiale documentario conservato presso l’Archivio Adriano Marabini della Cassa di Risparmio di Imola e grazie a una ricerca bibliografica specifica, questo saggio vuole tracciare un quadro della vita e della carriera dell’architetto. Inoltre, grazie alla presenza di documenti personali quali corrispondenza privata e fotografie, questa ricerca vuole dare voce non solo al professionista, ma anche all’uomo, con un duplice scopo: comprendere quanto le scelte di vita personale abbiano influenzato la sua opera architettonica, e collocare tale opera all’interno del più ampio quadro dell’architettura italiana del Novecento
«Un tirocinio aspro e ininterrotto». Architettura e vita di Adriano Marabini
No full textQuesto saggio è dedicato alla vita e alle opere dell’architetto Adriano Marabini. Dagli anni di formazione presso la Reale Accademia di Belle Arti di Bologna, passando per le prime esperienze professionali durante il Ventennio, fino all’intensa attività progettuale ed edilizia negli anni della ricostruzione, la lunga carriera di Marabini si intreccia con la sua vita personale, creando una complessa rete di relazioni che intercettano molti dei grandi temi dell’architettura italiana del Novecento. Tra questi, la costruzione di memoriali per la Grande Guerra e le architetture celebrative per il regime fascista, tra cui spicca la realizzazione della Casa del Fascio di Imola e alcuni progetti per servizi quali colonie montane e elioterapiche; i piani di ricostruzione urbana avviati nel secondo Dopoguerra, per i comuni di Camposanto (MO) e Solarolo (RA); i progetti di edilizia residenziale, dalle residenze private all’edilizia popolare del Villaggio “La Barca” di Bologna; l’architettura sacra postconciliare legata alla figura del cardinale Giacomo Lercaro; infine, la progettazione architettonica nell’Italia del boom, che include spazi per lo spettacolo, ovvero progetti e restauri di teatri e cinema, e spazi per il lavoro, quali stabilimenti industriali e uffici bancari. Se il baricentro geografico delle opere di Marabini ruota tra le città di Imola e Bologna, con realizzazioni che abbracciano anche la Romagna e le Marche, la sua esperienza tuttavia supera la dimensione locale e affronta tutte le grandi tappe del secolo breve. Ogni momento storico lascia infatti una traccia nella vita di Marabini: l’esperienza militare durante la Grande Guerra, l’affermarsi come professionista durante il regime fascista, il ruolo nei delicati anni della ricostruzione nel secondo Dopoguerra. Attraverso il ricco materiale documentario conservato presso l’Archivio Adriano Marabini della Cassa di Risparmio di Imola e grazie a una ricerca bibliografica specifica, questo saggio vuole tracciare un quadro della vita e della carriera dell’architetto. Inoltre, grazie alla presenza di documenti personali quali corrispondenza privata e fotografie, questa ricerca vuole dare voce non solo al professionista, ma anche all’uomo, con un duplice scopo: comprendere quanto le scelte di vita personale abbiano influenzato la sua opera architettonica, e collocare tale opera all’interno del più ampio quadro dell’architettura italiana del Novecento
Thymol and Thymus Vulgaris L. activity against UVA- and UVB-induced damage in NCTC 2544 cell line
No full textMany authors focused on the research of natural compounds in order to protect skin from indirect (UVA) and direct (UVB) ultraviolet radiation side effects. The aim of this study to evaluate the protective effect of a dry extract from T. vulgaris L. and of its major synthetic compound thymol (about 60%), against oxidative and genotoxic UVA and UVB damage. Experiments were reproduced in a low differentiated keratinocytes cell line (NCTC 2544) Cells were pretreated for 1h, in serum-free medium, with thymol (1μg/mL) or T. vulgaris L. (1.82μg/mL) then exposed to different UVA (8-24J/cm2) or UVB doses (0.016-0.72J/cm2). Immediately after the UV exposure the intracellular redox status was evaluated by ROS quantification and by LPO. Genotoxic aspects were evaluated 24h after the end of irradiations using the alkaline comet assay, the micronucleus formation assay and the immunostaining of phosphorylated H2AX histone protein (detected 1h after the end of UV exposure). Thymol and T. vulgaris L. extract inhibited ROS generation in UVA and UVB-irradiated cells. On the contrary, MDA formation was reduced only in UVA treated cells. Both agents decreased the DNA damage evaluated by the alkaline comet assay, but not in the micronucleus and H2AX tests probably because of the severity of damage (double strands) detected
Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage
No full textObjective: The aims of our study were to characterize ultraviolet (UV)A- and UVB-induced damages in a keratinocytes cell line (HaCaT), and to evaluate the protective capacities two plant-derivative compounds, namely Thymus vulgaris L leaf extract and thymol, its major component. A polyphenol rich diet has gained wide attention and it is now considered to be a protective agent for human skin, which can be over-exposed to environmental factors and in particular UV light.
Methods: Cells were pretreated for 1 h, in serum-free medium, with thymol (1 μg/ml) or Thymus vulgaris L (1.82 μg/ml) then exposed to different UVA (8-24 J/cm2) or UVB doses (0.016-0.72 J/cm2). Immediately after the UV exposure the intracellular redox status was evaluated by reactive oxygen species quantification and apoptotic events. Genotoxic aspects were evaluated 24 h after the end of irradiations using the alkaline comet assay and the immunostaining of phosphorylated H2AX histone protein (detected 1 h after the end of UV exposure).
Results: The pre-treatment of our experimental model with the two substances confirmed an antioxidant action and anti-apoptotic effect by reducing the cells percentage (sub-G1 phase). Furthermore, thymol and extract of Thymus vulgaris L were able to reduce genotoxic damage. The alkaline comet assay showed that the two substances were capable to decrease DNA damage. Also in this case, Thymus vulgaris L extract is more effective than thymol in decreasing genotoxicity markers.
Conclusions: Our results confirmed the more oxidant UVA and more genotoxic UVB effects. Regarding the protective effect of thymol and Thymus vulgaris L extract, data obtained proved their antioxidant and free-radical scavenging ability as known for phenolic (which our compounds belong to) and polyphenolic compounds. Thymol and mainly Thymus vulgaris L extract were also able to reduce the direct genotoxic damage
Goldman and Siegel on the epistemic aims of education
No full textPhilosophers have claimed that education aims at fostering disparate epistemic goals. In this paper we focus on an important segment of this debate involving conversation between Alvin Goldman and Harvey Siegel. Goldman claims that education is essentially aimed at producing true beliefs. Siegel contends that education is essentially aimed at fostering both true beliefs and, independently, critical thinking and rational belief. Although we find Siegel's position intuitively more plausible than Goldman's, we also find Siegel's defence of it wanting. We suggest novel argumentative strategies that draw on Siegel's own arguments but look to us more promising
DNA damage and oxidative stress in cell fish line RTG-2 after exposure to PCB mixture(PCB153,138, 101, 118)
No full textBoghossian's template and transmission failure
No full textWithin his overarching program aiming to defend an epistemic conception of
analyticity, Boghossian (1996 and 1997) has offered a clear-cut explanation of how we can acquire a priori knowledge of logical truths and logical rules through implicit definition. The explanation is based on a special template or general form of argument. Ebert (2005) has argued that an enhanced version of this template is flawed because a segment of it is unable to transmit warrant from its premises to the conclusion. This article aims to defend the template from this objection. We provide an accurate description of the type of non-transmissivity that Ebert attributes to the template and clarify why this is a novel type of non- transmissivity. Then, we argue that Jenkins (2008)’s response to Ebert fails because it focuses on doxastic rather than propositional warrant. Finally, we rebut Ebert’s objection on Boghossian’s behalf by showing that it rests on an unwarranted assumption and is internally incoherent
Protective effect of Vaccinium myrtillus extract against UVA- and UVB-induced damage in a human keratinocyte cell line (HaCaT cells)
No full textRecently, the field of skin protection have shown a considerable interest in the use of botanicals. Vaccinium myrtillus contains several polyphenols and anthocyanins with multiple pharmacological properties. The purpose of our study was to examine whether a water-soluble V. myrtillus extract (dry matter 12.4%; total polyphenols 339.3 mg/100 g fw; total anthocyanins 297.4 mg/100 g fw) was able to reduce UVA- and UVB-induced damage using a human keratinocyte cell line (HaCaT). HaCaT cells were pretreated for 1 h with extract in a serum-free medium and then irradiated with UVA (8-40 J/cm(2)) and UVB (0.008-0.72 J/cm(2)) rays. All experiments were performed 24 h after the end of irradiation, except for oxidative stress tests. The extract was able to reduce the UVB-induced cytotoxicity and genotoxicity (studied by comet and micronucleous assays) at lower doses. V. myrtillus extract reduced lipid peroxidation UVB-induced, but had no effect against the ROS UVB-produced. With UVA-induced damage V. myrtillus reduced genotoxicity as well as the unbalance of redox intracellular status. Moreover our extract reduced the UVA-induced apoptosis, but had no effect against the UVB one. V. myrtillus extract showed its free radical scavenging properties reducing oxidative stress and apoptotic markers, especially in UVA-irradiated cells
UV-B damage: a potential molecular play of Vitis vinifera L. extract
No full textUltraviolet component of sunlight UV-B (280-315 nm) is one of the major cause of skin damage. UV-B radiations have a low wavelength being absorbed almost completely by the epidermis; however, they interact directly with DNA causing molecular rearrangements. Photoaging and the development of skin cancer are of increasing relevance since lifestyle changes have led to an increase in the individual UV doses. Therefore, new prevention strategies have to be developed in order to reduce UV damage and delay photoaging process. In this context, different polyphenol-enriched botanicals have been proposed for the prevention of UV-mediated skin damage. Here it has been evaluated the antioxidant and DNA protective potential of an aqueous extracts of Vitis vinifera L., validated for the contents of anthocyanins, flavonoids and caffeic acid, against UV-B radiation in human keratinocyte (HaCaT) cell line. Treatment with the extract (100 μg/ml, 1h in serum-free media), was followed by the exposure to UV-B (20-30-40-80-160-320-640 mJ/cm2) radiation in PBS. The extract protects from the direct DNA damage induced by UV-B exposure even at doses where no oxidative damage was observed, as indicated by the alkaline comet and ɣH2AX tests, evaluated at T0 after the exposure. Moreover, it seems that the extract maintains the protective apoptotic pathway induced by UV-B. All these evidences led us to study the effect of the extract on DNA damage at molecular level, looking at the expression of genes involved in several pathways of DNA damage recognition, repair and apoptosis induction. Interestingly, the extract is able to modulate the expression of several genes involved both in damage signalling and NER (Nucleotide excision repair) pathway, mostly at 40 mJ/cm2 dose. The most important effect of the extract is on GADD45α especially at 40 and 80 mJ/cm2. This gene is pivotal in DNA repair pathway induced by UVR exposure, acting also on negative growth control. All together these findings suggest a potential interesting play of the extract also at the molecular level, modulating the expression of key genes involved in DNA damage signalling and repair
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