5,904 research outputs found
The pathway of cross-presentation is influenced by the particle size of phagocytosed antigen
No full textCross-presentation is the presentation by MHC class I of antigenic peptides from exogenous proteins that have been internalised and processed by professional antigen presenting cells, eg. dendritic cells (DC). We have investigated the influence of particle size and antigen load on cross-presentation following antigen delivery on microspheres (MS). Cross-presentation from small particles (0.8 ?m) is sensitive to proteasome inhibition and the blockade of ER-resident MHC class I complex export, whereas cross-presentation from larger particles (aggregated clumps of 0.8 ?m MS) is resistant to these antagonists. This observation may have been overlooked previously, due to the heterogeneity of particle size and MS uptake in unsorted DC populations. Whilst larger particles carry more antigen, we show that antigen load does not influence the cross-presentation pathway utilised. Whereas early endosome autoantigen 1 (EEA1) could be observed in all phagosomes, we observed endoplasmic reticulum SNARE of 24 kDa (ERS24) and cathepsin S in association with 3.0 ?m and aggregated 0.8 ?m MS, but not individual 0.8 ?m MS. A potential mechanism underlying our observations may be the activation of ?-catenin by disruption of E-cadherin-mediated adhesion. Activated ?-catenin was detected in the cytoplasm of cells after phagocytosis of MS (highest levels for the largest particles). We propose that particle size can direct the use of different pathways for the cross-presentation of an identical antigen. Furthermore, these pathways have differing yields of MHC class I-peptide complexes, which is an important variable in designing vaccination strategies for maximal antigen expression and CD8(+) T cell priming
Do dolphins benefit from nonlinear mathematics when processing their sonar returns?
No full textAn interview with author Tim Leighton about the paper
Opportunities for linking young surveyors across professional surveying member organisations and FIG
No full textTim Di Muzio on 'Sabotage'
No full textIn a series of essays published in 2013 and 2014 on capitaspower.com, political economist Tim Di Muzio explored the concept of ‘sabotage’ as it applies to capitalist power. I recently rediscovered these essays and was so impressed by them that I have reposted them here as a single piece.
About the author: Tim Di Muzio is a researcher at the University of Wollongong. He is the author of numerous books, including Debt as power, Carbon capitalism, and The 1% and the Rest of us
1996-1997 Tim Gautreaux
No full textTim Gautreaux is the author of three novels and two earlier short story collections. His work has appeared in The New Yorker, The Best American Short Stories, The Atlantic, Harper’s, and GQ. After teaching for thirty years at Southeastern Louisiana University, he now lives, with his wife, in Chattanooga, Tennessee. (Photo credit: Randy Bergeron)https://egrove.olemiss.edu/grisham_res/1023/thumbnail.jp
First person - Tim Petzold
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping researchers promote themselves alongside their papers. Tim Petzold is first author on ‘ Connexin 41.8 governs timely haematopoietic stem and progenitor cell specification’, published in BiO. Tim conducted the research described in this article while a PhD student in Julien Bertrand's lab at the Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Switzerland. He is now a postdoc in the lab of Holger Gerhardt at the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, investigating developmental biology – previously his focus was on how blood stem cells develop and now it has shifted to how the vascular system develops
Tim Seibles, 40th Annual ODU Literary Festival
No full textTim Seibles is the author of several poetry collections including Hurdy-Gurdy, Hammerlock, Buffalo Head Solos, and Fast Animal, which was a finalist for the 2012 National Book Award. In 2013 he received both the Pen Oakland Josephine Miles Award for poetry and an honorary Doctorate of Humane Letters from Misericordia University for his literary accomplishments. His latest collection, One Turn Around the Sun, has just been released. Tim is the current Poet Laureate of Virginia and is a Professor of English at Old Dominion University where he teaches literature as well as classes in the MFA in writing program
Tim Seibles, 39th Annual ODU Literary Festival
No full textTim Seibles is the author of several poetry collections including Hurdy-Gurdy, Hammerlock, Buffalo Head Solos, and Fast Animal, which was a finalist for the 2012 National Book Award. In 2013 he received both the Pen Oakland Josephine Miles Award for poetry and an honorary Doctorate of Humane Letters from Misericordia University for his literary accomplishments. His latest collection, One Turn Around the Sun, has just been released. Tim is the current Poet Laureate of Virginia and is a Professor of English at Old Dominion University where he teaches literature as well as classes in the MFA in writing program
Global Media Ideas - Infinite Pathways to Creative Succes - Tim Chang - Part One.mp4
No full textDuring the X Media Lab: Global Media Ideas summit in June 2011 media and technology writer Brad Howarth conducted interviews with industry experts for Creativeinnovation. This video is part one of Brad Howarth's interview with Tim Chang about his role as Partner at Norwest Venture Partners (Palo Alto). Tim focuses on investments in mobile, gaming, digital media, and also leads Norwest Venture Partners's investment practice in China and Asia-Pacific. Tim shares tips on how to get an introduction to a Venture Capital; the elements of a good pitch and follow-up. And what he looks at when considering a deal - The 3Ts: Team, Traction, Tier 1 co-investors
Polymer microarrays: identification of substrates for phagocytosis assays
No full textA polymer microarray of 120 polyurethanes was used to identify polymers that promoted the adhesion of bone marrow dendritic cells (BMDC). Identified polymers were coated onto glass cover slips and shown to be efficient substrates for the immobilisation of these primary cells, which underwent efficient phagocytosis while still presumably maintaining their immature stat
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