9 research outputs found
Research Progress on Mechanical Behavior of Closed-Cell Al Foams Influenced by Different TiH2 and SiC Additions and Correlation Porosity-Mechanical Properties
No full textClosed-cell aluminium foams with different compositions have been manufactured starting from powders and also characterized from a morphological point of view and by means of compressive tests in order to determine mechanical properties. Circularity, equivalent diameter, and average porosity area of such foams have been calculated from the analysis of cross-sections as well specific energy absorption in compression tests. Samples with a higher amount of blowing agent (TiH2) have the highest energy absorption while samples with a higher amount of stabilizing agent (SiC) exhibit good foam properties overall (best compromise between morphology and energy absorption). The analysis of morphological properties, such as area, circularity, and equivalent diameter, can provide a better understanding of the foam’s structure and porosity-parameters which can be manipulated to enhance the foam’s properties for specific applications, both structural and functional
Streptococcus pneumoniae serotype prevalence and antibiotic resistance among young children with invasive pneumococcal disease: experience from a tertiary care center in South India
No full textIntroduction: We performed a study to describe the clinical profile, antimicrobial susceptibility and prevalent serotypes of pneumococcal isolates from children with suspected invasive pneumococcal disease (IPD) admitted to a tertiary care hospital in South India. Methods: Hospitalized children, ≤5 years with fever (>38 °C); increased respiratory rate or neurological symptoms were recruited, (as part of the Alliance for Surveillance of Invasive Pneumococci – ASIP – project) from January 2011 to March 2013. Identification of pneumococcal isolates from blood or cerebrospinal fluid samples was done by routine culture methods. Isolates were analysed for antimicrobial susceptibility, and confirmed by serotyping (using Quellung’s test) and multiplex PCR. Results: Out of the 171 samples received in the lab, 17 grew pneumococci identified by standard methods. Fourteen of them were confirmed by multiplex PCR. Maximum recruitment was observed during the months of January and February (36.4%, 28.6%). The average age of affected subjects was 21 months. The common clinical presentation was pneumonia (42.8%). Two isolates belonging to the 19F and 19B serotypes were resistant to penicillin (on Etest). The observed serotype distribution was 6B and 19F (2 each), and 1, 2, 6A, 9V, 10A, 14, 15A, 19B, 21, 35F (1 each). The overall fatality rate was 14.3% (n = 2); the S. pneumoniae isolates from these two patients belonged to the non-vaccine serotype 19B and serotype 19F and demonstrated in vitro resistance to penicillin and erythromycin. Conclusion: Our study demonstrates the presence of serotypes not covered by available vaccines in a pediatric cohort. Emergence of drug resistant Streptococcus pneumoniae may by associated with severe clinical outcomes
‐cymene complexes of chiral aroylthiourea ligands derived from unprotected D/L‐alanine as proficient catalysts for asymmetric transfer hydrogenation of ketones
No full textSynthesis, structures and mechanistic pathways of anticancer activity of palladium(<scp>ii</scp>) complexes with indole-3-carbaldehyde thiosemicarbazones
No full textPalladium(ii) complexes featuring bidentate heterocyclic thiosemicarbazones have been synthesized, characterized and evaluated for their biomolecular interactions. The complexes induced in vitro anticancer activity through apoptosis.</p
Water-Soluble Mono- and Binuclear Ru(η<sup>6</sup>-<i>p</i>-cymene) Complexes Containing Indole Thiosemicarbazones: Synthesis, DFT Modeling, Biomolecular Interactions, and <i>In Vitro</i> Anticancer Activity through Apoptosis
No full textErratum: Dual HER2 blockade: preclinical and clinical data
No full textAfter publication of our review [1], we noted errors to the legend of Figure 1B, C. The ado-trastuzumab-emtansine concentration should be 1 μg/ml instead of 1 mg/ml. The trastuzumab concentration should be 10 μg/ml instead of 10 mg/ml. The lapatinib concentration should be 10 μM instead of 10 mM (Please see Figure 1, a corrected version of the original Figure 1). Author detail
Water-Soluble Mono- and Binuclear Ru(η<sup>6</sup>‑<i>p</i>‑cymene) Complexes Containing Indole Thiosemicarbazones: Synthesis, DFT Modeling, Biomolecular Interactions, and <i>In Vitro</i> Anticancer Activity through Apoptosis
No full textIndole
thiosemicarbazone ligands were prepared from indole-3-carboxaldehyde
and N-(un)substituted thiosemicarbazide. The Ru(η6-p-cymene) complexes [Ru(η6-p-cymene)(HL1)Cl]Cl (1) and [Ru(η6-p-cymene)(L2)]2Cl2 (2*) were exclusively synthesized from thiosemicarbazone
(TSC) ligands HL1 and HL2, and [RuCl2(p-cymene)]2. The compounds were characterized by analytical
and various spectroscopic (electronic, FT-IR, 1D/2D NMR, and mass)
tools. The exact structures of the compounds (HL1, HL2, 1, and 2*) were confirmed by single-crystal X-ray diffraction
technique. In complexes 1 and 2*, the ligand
coordinated in a bidentate neutral (1)/monobasic (2*) fashion to form a five-membered ring. The complexes showed
a piano-stool geometry around the Ru ion. While 2* existed
as a dimer, 1 existed as a monomer, and this was well
explained through free energy, bond parameter, and charge values computed
at the B3LYP/SDD level. The intercalative binding mode of the complexes
with calf thymus DNA (CT DNA) was revealed by spectroscopic and viscometric
studies. The DNA (pUC19 and pBR322 DNA) cleavage ability of these
complexes evaluated by an agarose gel electrophoresis method confirmed
significant DNA cleavage activity. Further, the interaction of the
complexes with bovine serum albumin (BSA) was investigated using spectroscopic
methods, which disclosed that the complexes could bind strongly with
BSA. A hemolysis study with human erythrocytes revealed blood biocompatibility
of the complexes. The in vitro anticancer activity
of the compounds (HL1, HL2, 1, and 2*) was
screened against two cancer cell lines (A549 and HepG-2) and one normal
cell line (L929). Interestingly, the binuclear complex 2* showed superior activity with IC50 = 11.5 μM, which
was lower than that of cisplatin against the A549 cancer cell line.
The activity of the same complex (IC50 = 35.3 μM)
was inferior to that of cisplatin in the HepG-2 cancer cell line.
Further, the apoptosis mode of cell death in the cancer cell line
was confirmed by using confocal microscopy and DNA fragmentation analysis
Erratum: The Cancer Genome Atlas Comprehensive Molecular Characterization of Renal Cell Carcinoma (Cell Reports (2018) 23(1) (313–326.e5) (S2211124718304364) (10.1016/j.celrep.2018.03.075))
No full text(Cell Reports 23, 313–326; April 3, 2018) In the originally published version of this article, the author list contained two errors. Specifically, David J. Kwiatkowski was misspelled as David J. Kwaitkowski, and William Y. Kim was inadvertently written as William T. Kim. Both names have been corrected online. The authors regret this error
Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase
No full textN-terminal acetylation is an abundant modification influencing protein functions. Because ∼80% of mammalian cytosolic proteins are N-terminally acetylated, this modification is potentially an untapped target for chemical control of their functions. Structural studies have revealed that, like lysine acetylation, N-terminal acetylation converts a positively charged amine into a hydrophobic handle that mediates protein interactions; hence, this modification may be a druggable target. We report the development of chemical probes targeting the N-terminal acetylation-dependent interaction between an E2 conjugating enzyme (UBE2M or UBC12) and DCN1 (DCUN1D1), a subunit of a multiprotein E3 ligase for the ubiquitin-like protein NEDD8. The inhibitors are highly selective with respect to other protein acetyl-amide-binding sites, inhibit NEDD8 ligation in vitro and in cells, and suppress anchorage-independent growth of a cell line with DCN1 amplification. Overall, our data demonstrate that N-terminal acetyl-dependent protein interactions are druggable targets and provide insights into targeting multiprotein E2-E3 ligases.</p
