57 research outputs found
Nuovi paesaggi per la campagna urbana di Palermo
No full textStudi per la tutela del territorio e del paesaggio del ponente palermitano
PML promotes metastasis of triple-negative breast cancer through transcriptional regulation of HIF1A target genes
No full textElucidating the molecular basis of tumor metastasis is pivotal for eradicating cancer-related mortality. Triple-negative breast cancer (TNBC) encompasses a class of aggressive tumors characterized by high rates of recurrence and metastasis, as well as poor overall survival. Here, we find that the promyelocytic leukemia protein PML exerts a prometastatic function in TNBC that can be targeted by arsenic trioxide. We found that, in TNBC patients, constitutive HIF1A activity induces high expression of PML, along with a number of HIF1A target genes that promote metastasis at multiple levels. Intriguingly, PML controls the expression of these genes by binding to their regulatory regions along with HIF1A. This mechanism is specific to TNBC cells and does not occur in other subtypes of breast cancer where PML and prometastatic HIF1A target genes are underexpressed. As a consequence, PML promotes cell migration, invasion, and metastasis in TNBC cell and mouse models. Notably, pharmacological inhibition of PML with arsenic trioxide, a PML-degrading agent used to treat promyelocytic leukemia patients, delays tumor growth, impairs TNBC metastasis, and cooperates with chemotherapy by preventing metastatic dissemination. In conclusion, we report identification of a prometastatic pathway in TNBC and suggest clinical development toward the use of arsenic trioxide for TNBC patients
Trioctahedral smectites and zeolites as transformation products of the Cala Pozzolana di Ponente pyroclastic rocks (Linosa Island - Italy)
No full textOn Linosa Island the pyroclastites of Cala Pozzolana di Ponente have been affected by post depositional alteration phenomena. Field and laboratory study point out that this pyroclastic deposit was affected by hydrothermal circulation with formation of secondary minerals like smectite, zeolites, calcite. The transformation processes were accomplished in two stages; a first one was brought about by the circulation of abundant hydrothermal fluids that reacted with the glass fragments producing a thin regular crust of smectite surrounding glass fragments and a second one, responsible for the zeolite growth, was accomplished by fluids enriched in alkalis and calcium released by the altered glass, in conditions of decreasing fluid circulation and falling temperature. -from Author
Il Parco Pedemontano di Ponente (Riserva Reale di Boccadifalco ed aree limitrofe): alcune motivazioni e considerazioni
No full textSaggio sul recupero a Parco della riserva reale di Boccadifalco a Palerm
Enhanced recording paradigms and advanced analyses of peripheral nerve fibers SPiike software
No full textPrograma de Doctorat en BiomedicinaThe aim of this work is to investigate the human nociceptive system at the peripheral level. Researchers are still debating how the pain perception arises from this very intricate network. The human perception is the most elusive part of our knowledge since different subsystems are involved. The external information such as noxious stimuli must be processed at the peripheral level and through signal cascades and transduction this signal must reach the brain. At the brain level the information is processed and some decisions are taken, such as the well-known fight-or-flight response.
In the introduction, the author describes how the human nociceptive system works and in which way the noxious stimulus is converted into a signal understandable by the brain. Several cortical and subcortical areas are involved in this signal processing and going deeper in this assembly line the information becomes more abstracted. The whole pathway is fundamental for pain perception, however some diseases start at the peripheral level. This in turn makes wrong signals reaching the brain. The brain is then processing information that are not real and the responses do not suit with the needs. Therefore, the peripheral system must be investigated and understood firstly, since some central diseases may have a peripheral component as well. With this purpose in mind the microneurography technique has been used. This technique has got some complexity and a computer-aided system must be implemented. The hardware aims to filter out the noisy signal and perform recording and stimulation of the neural fibers. The software is instead used to make the stimulation and recording as automatic as possible in a way that researchers do not have to deal with a lot of parameters and steps to carry out this powerful but also time consuming technique. Some software are already available in the market however even if they work fine with slow conduction fibers such as C-fibers they cannot cope with faster neurons (e.g. Aδ fibers). The aim of this work is to create a software (i.e. SPiike) able to stimulate and record every type of fibers implementing advanced analysis technique as well. Furthermore, considering that some in vivo experiments have been pursued within the project to check the functionality of the software, more specifically in rats and mice, the comparison between human nociceptors and mouse nociceptors is depicted in this section.
In the method section, the experimental approach is described step by step. This is composed by several systems that work together for the stimulation, recording and analysis of the neural fibers. The control and acquisition module is composed by the software and a data acquisition board that trigger the stimulator and record the filtered signal. The stimulation module is composed by a stimulator that can be tuned as wish through dedicated knobs. Then the stimulus is delivered to the animal model (or the human patient) and the signal is recorded though a microelectrode inserted into the sciatic nerve. The amplification module is filtering out the noisy signal and is feeding a audio monitor for helping the researcher during the insertion of the electrode inside the nerve and it provides support during the whole experiment giving insights on fiber discharges. In this section the whole setup is described in details as well as the devices needed for the recording. Furthermore, the software development that is the core of this project is described as well, with all the considerations that must be considered during coding. Indeed, the flow chart must be followed methodically in order to minimize bugs and errors that may arise in the final product. Thus a description of the compiler and the Matlab IDE is given along with system and software requirements for the making of the SPiike software. Eventually the explanation of embedded functionalities and capabilities of SPiike is depicted in the final part of this section. This software is indeed able to stimulate slow conducting fibers as well as faster ones, and enhanced analysis techniques such as supervised machine learning are implemented.
In the results section, the graphical user interface of the Spiike software is reveled. It resembles the one of another software already available in the market, with a filtered signal and a raster plot embedded on it. However, this software is more user-friendly and it accounts with icons and drop-down menus that enhance the experience of the users during the use of the tool, making their interactions smooth and intuitive. The SPiike software is subdivide into two different tools, a recording module and a analysis module. The former allows the stimulation and recording of neural fibers with a stimulation frequency up to 1000Hz and some online analysis can be conducted to have insights on fibers type and behavior. The analysis module is instead a more powerful analysis environment that can retrieve the dataset recorded with the other module or with the LabChart software. Advanced analysis techniques are implemented in this module, this is meant to speed up fiber classification and analysis.
Conclusion and discussion provide a overview on some results. These will be compared to those obtainable through other software available in the market. In this section, pros and cons of the new implemented software, SPiike, will be described as well
Un parco possibile: luoghi e società tra urbanistica e paesaggio
No full textLe trasformazioni possibili del ponente palermitano tra paesaggio, urbanistica e progetto del territorio. In particolare il territorio di Boccadifalco porta con sé numerose istanze da recuperare e valorizzare, sul piano vegetale e naturalistico, sul piano architettonico e monumentale. Nell'articolo e nel volume sono tratteggiati alcuni modelli possibili ed alcune esperienze condotte nella direzione del recupero e della tutela di aree urbane non edificate, anche con vocazioni e caratteri differenti, che hanno costituito ed incrementato la dotazione di verde di alcune città italiane ed europee. Per volere citare qualche esempio di città che possano somigliare al caso di Palermo per dimensione, ruolo e struttura, abbiamo già ricordato l’esperienza di Milano (Parco Nord e Boscoincittà), ma in sede nazionale possiamo aggiungere l’esperienza di Roma, che attraverso la sua Agenzia parchi “Romanatura”, promuove e coordina la gestione di undici aree a parco dentro e attorno la capitale, e anche in questo caso nei diversi esempi per taglia e collocazione, si riscontrano diversi caratteri (da quello archeologico a quello agrario e naturalistico)
Génova y su frontera marítima entre los siglos XVI y XVIII: defensa y control
No full textCorsa barbaresca (nella duplice forma dell’incursione a terra e dell’azione predatrice in mare), contrabbando, controlli di Sanità, difesa del territorio dagli appetiti delle potenze europee, tutela della giurisdizione sul mar Ligure di fronte all’attività dei corsari stranieri. Queste sono le principali problematiche che Genova deve affrontate durante i secoli dell’età moderna lungo le sue estesissime e variegate frontiere marittime. E non mi riferisco solo alle due Riviere liguri e alle coste della Corsica, ma anche agli ampi spazi marittimi compresi in quel triangolo che ha i propri vertici in Monaco a ponente, in Capocorvo a levante e in Bonifacio a sud. Di fronte alla minaccia corsara, ad un contrabbando presente in forma endemica e particolarmente florido, alle emergenze di Sanità, e all’invadente presenza di alcune potenze europee la Repubblica reagisce attraverso strumenti di difesa e controllo sia terrestri che navali. Si va dalla realizzazione di un cordone di torri e fortificazioni che abbraccia le coste liguri e còrse nella loro interezza, all’impiego delle galee degli assentisti al servizio della Spagna e dello stuolo pubblico, alla mobilitazione straordinaria delle risorse locali, sia in terra che in mare. Il tutto in una prospettiva dinamica, caratterizzata da alcuni intensi mutamenti di carattere strategico: il venire meno della secolare alleanza con gli Asburgo di Spagna alla fine del Seicento e lo scoppio, nel 1729, di una quarantennale sollevazione in quella Corsica il cui controllo, insieme a quello della rada di Vado e del golfo della Spezia, era considerato fondamentale per la sicurezza del Dominio di Terraferma e di Genova stess
Supplementary Figure 4 from Synergistic Leukemia Eradication by Combined Treatment with Retinoic Acid and HIF Inhibition by EZN-2208 (PEG-SN38) in Preclinical Models of PML-RARα and PLZF-RARα–Driven Leukemia
No full textSupplementary Figure 4: Combined effect of EZN-2208 and ATRA on NB4 cell viability and differentiation.</p
El llamado "foro del discapacitado". Análisis de la nueva ley de patrimonio protegido de las personas con discapacidad
No full textThis paper presents the most controversial points of the new Law of Protected Patrimony of Persons with Discapacity. The author expresses and reviews the novelties implied by this law and its consequences in practice, contributing his opinions with regard to its suitability.En la presente ponencia, se presentan los puntos más controvertidos de la nueva Ley del Patrimonio Protegido de las personas con discapacidad. El ponente expresa y repasa las novedades que esta ley implica y sus repercusiones en la práctica, aportando sus opiniones al respeto de su idoneidad
Supplementary Figure 3 from Synergistic Leukemia Eradication by Combined Treatment with Retinoic Acid and HIF Inhibition by EZN-2208 (PEG-SN38) in Preclinical Models of PML-RARα and PLZF-RARα–Driven Leukemia
No full textSupplementary Figure 3: EZN-2208 affects leukemic stromal cell numbers.</p
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