1,726,420 research outputs found
Interview with Mandeep Mann and Setayesh Yazdani
No full textMandeep Mann and Setayesh Yazdani are researchers in the Department of Pharmacology and Toxicology at the University of Toronto under the supervision of Dr. Matthieu Schapira, the head of research informatics at the Structural Genomics Consortium (SGC). Mandeep is a PhD candidate in the Department of Pharmacology and Toxicology, and Setayesh recently completed her MSc in the same department. Setayesh is currently a Doctor of Pharmacy student at the Leslie Dan Faculty of Pharmacy at the University of Toronto
PERANCANGAN CORPORATE IDENTITY BAKSO MALANG MANDEEP
No full textManusia tidak lepas dari makanan, setiap manusia membutuhkan makanan yang menghasilkan sumber energi untuk melakukan semua kegiatan. Makanan di setiap daerah sangat beragam dan mempunyai ciri khas masing-masing. Hal ini juga berpengaruh dengan letak geografis, ekonomi, dan hasil bumi yang didapat pada daerah tertentu, misalnya penduduk yang memiliki hasil bumi berupa kentang akan membuat kentang sebagai makanan pokoknya.
Bakso merupakan salah satu makanan yang banyak disukai. Beragam jenis bakso banyak beredar di pasaran sehingga dapat memudahkan para penikmat bakso untuk mencobanya. Bakso malang adalah salah satu jenis bakso yang terdapat di indonesia. Di Bandung khususnya banyak sekali restoran atau tempat makan hingga gerobak yang menjual bakso jenis ini. Hal ini juga menandakan bahwa minat konsumen di Bandung atas bakso malang sangat besar. Bakso Malang Mandeep adalah salah satu jenis usaha bakso malang yang turut meramaikan penjualan bakso di Bandung. Bakso Malang Mandeep menyajikan rasa bakso yang istimewa namun dengan harga yang lebih terjangkau oleh berbagai kalangan.
Usaha ini dirintis oleh Bapak Syaeful dari mulai berjualan bakso dengan gerobak sejak tahun 1980-an, sampai berupa warung dan sekarang sudah memiliki tujuh cabang. Dan sudah bekerja sama dengan berbagai pihak untuk mengembangkan usahanya. Persaingan yang keras dalam dunia usaha sekarang ini menyebabkan pentingnya setiap badan usaha untuk memiliki Corporate Identity, termasuk badan usaha yang bergerak dalam jenis usaha bakso malang ini. Corporate Identity untuk jenis usaha ini tidak hanya berfungsi sebagai identitas dan pembeda dari bakso malang lainnya,namun juga berfungsi sebagai penanaman citra atau image agar bisa menarik konsumen dan mempertahankanya agar konsumen tetap ingat pada bakso malang terserbut.
Berdasarkan latar belakang tersebut, penulis berusaha untuk melakukan penelitian terhadap Bakso Malang Mandeep, mengenai perancangan Corporate Identity. Oleh karena itu penulis mengambil sebuah judul yang berhubungan dengan Bakso Malang Mandeep yaitu :PERANCANGAN CORPORATE IDENTITY BAKSO MALANG MANDEE
National Dengue Day (India): Combatting the Vector-Borne Public Health Threat
Get PDFGuest comment by Dr. Mandeep Singh on the occasion of National Dengue Day (India) observed on 16th May, 202
Mandeep Matharu's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
Mandeep Matharu's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
Characterization of Mouse and Human Astrocytes in Amyotrophic Lateral Sclerosis: Effects of Oxidative Stress and Blockade of the Metabotropic Glutamate Receptor 5
No full textAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder due to upper and lower motor neuron (MNs) death. Recognized as a non-cell-autonomous disease, ALS is also characterized by damage and degeneration of glial cells, such as astrocytes, microglia, and oligodendrocytes. Astrocytes acquire a reactive and toxic phenotype defined by an abnormal proliferation and by the release of neurotoxic factors.
Recent studies reported that the uptake of [18F]-fluorodeoxyglucose (FDG) is increased in the spinal cord (SC) and decreased in the motor cortex (MC) of patients with ALS, suggesting that the disease might differently affect the two nervous districts with different time sequence or with different mechanisms. Here we show that MC and SC astrocytes harvested from newborn B6SJL-Tg (SOD1G93A) 1Gur (SOD1G93A) mice could play different roles in the pathogenesis of the disease. Spectrophotometric and cytofluorimetric analyses showed an increase in redox stress, a decrease in antioxidant capacity, and a relative mitochondria respiratory uncoupling in MC SOD1G93A astrocytes. By contrast, SC mutated cells showed a higher endurance against oxidative damage, through the increase in antioxidant defense and a preserved respiratory function. Thus, SOD1G93A mutation differently impaired MC and SC astrocyte biology in a very early stage of life.
One major cause for MN degeneration in ALS is represented by glutamate-mediated excitotoxicity, due to the alteration of glutamate transmission mechanisms, including glutamate receptor function. In this context, the Group I metabotropic glutamate receptor 5 (mGluR5) has been proposed to play an important role in ALS, since it is largely overexpressed during disease progression and is involved in the altered neuronal and glial cellular processes. My research group previously demonstrated that mGluR5 produces abnormal glutamate release in the spinal cord of the SOD1G93A mouse model of ALS and that halving its expression has a positive impact on in-vivo disease progression, including motor neuron survival, astrogliosis, and microgliosis. They also investigated the consequences of reducing the mGluR5 expression in SOD1G93A mice on the reactive phenotype of spinal cord astrocytes cultured from late symptomatic (120 days old) SOD1G93A mice. Also in this model, reducing the mGluR5 expression ameliorated the astrocyte phenotype.
UNIVERSITY OF GENOVA 8
Here, I translated this study to human astrocytes derived from healthy donors and ALS patients. We investigated the in-vitro pharmacological treatment effect of chloro-4-((2,5-dimethyl-1-(4-(trifluoromethoxy)phenyl)-1H-imidazol-4-yl)ethynyl)pyridine (CTEP), a negative allosteric modulator of mGluR5 on i-astrocytes differentiated from the inducible neural progenitor cells (iNPCs) obtained from the skin fibroblast (i-astrocytes) of two ALS patients and two healthy donors. The overexpression of anti-glial fibrillary acid protein (GFAP), S100 calcium-binding protein β (S100β), and Complement component 3 (C3), three markers of astrogliosis, was reduced in CTEP-treated i-astrocytes. The same positive effect was obtained in the case of NLR family pyrin domain containing 3 (NLRP3) and nuclear factor erythroid 2-related factor 2 (NRF2), markers strictly related to inflammation and oxidative stress respectively, which are upregulated in ALS astrocytes. In-vitro pharmacological treatment with CTEP also reduced the expression of mGluR5 in mutated i-astrocytes. In addition, the CTEP treatment caused a decrement in antioxidant enzymatic activity such as malondialdehyde (MDA), glucose-6-phosphate dehydrogenase (G6PD), Glutathione reductase (GR), Glutathione peroxidase (GP), and catalase compared to the untreated samples, suggesting that the drug could cause a reduction of oxidative stress.
Altogether, these results indicate that reduction of mGluR5 activation has a positive impact on i-astrocytes in ALS patients supporting the idea that the in-vivo amelioration of the disease progression, registered after mGluR5 genetical or pharmacological silencing, involve an astrocyte phenotype improvement also in humans. As a whole, mGluR5 may represent a potential therapeutic target to preserve MNs from death, also by modulating the reactive astroglial phenotype in ALS
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