2,535 research outputs found
Functional characterization of MuSK, receptor tyrosine kinase required for the formation and the maintenance of nerve-muscle synapses : "in vivo" and "in vitro" approaches
No full textThe developing neuromuscular junction (NMJ) serves as one of the best model systems for studying synapse formation since changes in shape, size, and molecular composition can be followed with high spatial and temporal resolution. Formation of the NMJ depends on coordinated interactions between nerve terminals and muscle fibres [1] and requires reciprocal signals from both cells to efficiently regulate all the events taking place during its development. This includes synapse-specific gene expression, generation of action potentials and stabilization events leading to the formation of a sophisticated apparatus which ensures that the muscle fibre is provided with trophic factors as well as electrical stimuli. The receptor tyrosine kinase MuSK and its natural ligand, a neuron-specific isoform of the extracellular matrix molecule agrin, are considered to play a fundamental role in the formation and maintenance of the NMJ. In cultured myotubes, MuSK is activated by neural agrin, and this causes its phosphorylation and results in the formation of AChRs clusters on the cell surface [2-5]. The present study discusses different approaches to understand better the mechanisms of how the NMJ is formed and maintained. In the first project, we addressed the question of MuSK – neural agrin interaction and the necessity for an additional component of the agrin receptor complex. We generated transgenic mice overexpressing MuSK or neural mini-agrin as well as both proteins throughout the entire muscle fibre. We found evidence that in muscle cells MuSK is sufficient to respond to neural agrin with no necessity of any additional co-receptor protein. We also show that Dok-7, a MuSK adaptor protein, limits the formation of ectopic postsynaptic like structures in innervated muscle. From this, we conclude that it is very likely that in muscle cells MuSK serves as a functional receptor for neural agrin. The second project refers to the regulation of the NMJ formation. We found that signal transduction downstream of agrin involves the mitogen-activated protein kinase (MAPK) pathway, particularly ERK1/2 and JNK. It involves MuSK signaling, requires Dok7 and is ErbB-independent. We also show that MAPK phosphatase-1, MKP-1, plays a crucial regulatory role in formation of the nerve-muscle connection. Results of the third project describe that a miniaturized form of agrin is able to fully rescue perinatal death of agrin-deficient mice, and that this function does not depend on local deposition of agrin at synapses. Moreover, we show that acetylcholine together with neural agrin stabilizes the postsynaptic structures at the NMJ. The function of agrin in CNS was our main interest in the fourth project. Using
agrin-deficient mice with a transgenic reconstitution of the expression of neural agrin by motor neurons, we found that in the brain, agrin is localized to the excitatory synapses. Lack of agrin resulted in a strong reduction of synaptic structures in the cerebral cortex coinciding with the attenuation of the frequency of miniature postsynaptic currents. Additionally we found that muscle specific kinase MuSK is also expressed in the brain, thus possibly involved in the formation of the nerve-nerve connections. Finally, we show that agrin function involves MAP kinase signaling
Processed measurement data of acceleration of MR fluid-filled cushion gripper on UR3e robot
No full textThe dataset is a supplement to the article that will soon be submitted for review. DESCRIPTION:The dataset consists of Absolute Orientation Sensor (BNO055) measurements during the transportation of an object by a UR3e robot. The trajectory of the robot's movement includes lifting the object to a height of about 200 mm, horizontal displacement for a distance of 400 mm, and lowering by 200 mm. The transfer procedure begins with the closing of the jaws and ends with their opening. The procedure is analogous to the one outlined in the following dataset:Białek, Marcin, 2023, "Processed measurement data on grip forces during object transportation by a robot using gripper with a MR fluid-filled cushions", https://doi.org/10.18150/B2KCKC, RepOD.The experiment was conducted for three values of acceleration and velocity of the robot joints. For each such configuration, 5 handling tests were carried out.LICENSE:The data are under Creative Commons License CC BY. It is though recommended to manipulate along with the author to fully understand the outcomes. If you have any questions do not hesitate to contact: marcin.bialek@put.poznan.plThis research was funded by the National Science Centre, Poland, grant number: 2021/41/N/ST8/02619. https://ror.org/03ha2q922FILE:"dataset.csv" - processed measurement data obtained during the experiment.For proper interpretation, please refer to the images provided in readme files.COLUMNS: - R_Speed[deg/s] - robot joint movement velocity;- R_Acc[deg/s^2] - robot joint movement acceleration;- SAMPLE[-] - sample number. For each object configuration, acceleration and speed, 5 trials were conducted;- TIME[s] - the measurement time at which the force was recorded;- Linear_X[m/s^2] - X axis of linear acceleration data (acceleration minus gravity)- Linear_Y[m/s^2] - Y axis of linear acceleration data (acceleration minus gravity)- Linear_Z[m/s^2] - Z axis of linear acceleration data (acceleration minus gravity)- Orient_X[deg] - X axis orientation data based on a 360° sphere- Orient_Y[deg] - Y axis orientation data based on a 360° sphere- Orient_Z[deg] - Z axis orientation data based on a 360° sphere- Accl_X[m/s^2] - X axis of acceleration (gravity + linear motion)- Accl_Y[m/s^2] - Y axis of acceleration (gravity + linear motion)- Accl_Z[m/s^2] - Z axis of acceleration (gravity + linear motion)</p
Autoethnography, Storytelling, and Life as Lived: A Conversation Between Marcin Kafar and Carolyn Ellis
No full textThis conversation takes place in Warsaw. Carolyn Ellis has come to Poland to accompany Jerry
Rawicki, a Warsaw Ghetto survivor, on his first trip back to Poland since the Holocaust. There
she arranged to meet Marcin Kafar, a scholar in Poland who has spent time with her at the University of South Florida in Tampa, Florida. During this visit, Marcin assists Carolyn with video
recording Jerry’s experiences as they visit Holocaust sites, and Jerry remembers and reflects on
his experience. Afterwards, Marcin converses with Carolyn about autoethnography, storytelling,
and the importance of life in the context of searching for ethos by academics
PLoS Open Peer Review Corpus
No full textPLoS Open Peer Review CorpusSection for Logic & Cognitive Science, Institute of Philosophy and Sociology, Polish Academy of ScienceGenerated by Ksawery Jasieński under supervision of Marcin Miłkowski (2022)Public Library of Science (PLoS) journals are committed to open peer review idea, but these are voluntary. They are not available for download in a single package, but they are contained in their huge data dump.The data set contains all available peer reviews as of 16 June 2022 (138 papers with reviews). In addition, the corpus contains metadata about particular reviews, author responses, decision letters and paper metadata in the JSON format. The JSON schema files are available in the schema subdirectory in files with self-explanatory names.The original files were not enriched with any linguistic annotation or converted to any format (these are predominantly PDF, TXT, and DOCX files, as uploaded through the MDPI editorial system by reviewers).Additionally, we are making the filter code available, in the review_crawler directory. For PLoS reviews, plos_crawler.py should be used (more notes in the subdirectory).The files are being made available under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).</p
MDPI Open Peer Review Corpus
No full textMDPI Open Peer Review CorpusSection for Logic & Cognitive Science, Institute of Philosophy and Sociology, Polish Academy of ScienceGenerated by Ksawery Jasieński under supervision of Marcin Miłkowski (2022)MDPI is committed to open peer review idea, but these are voluntary. They are not available for download in a single package, so they must be crawled from their website.The data set contains all available peer reviews as of 16 June 2022 (123 papers with reviews). In addition, the corpus contains metadata about particular reviews, author responses and paper metadata in the JSON format. The JSON schema files are available in the schema subdirectory in files with self-explanatory names.The original files were not enriched with any linguistic annotation or converted to any format (these are predominantly PDF, TXT, and DOCX files, as uploaded through the MDPI editorial system by reviewers).Additionally, we are making the crawler code available, in the review_crawler directory. For MDPI reviews, mdpi_crawler.py should be used (more notes in the subdirectory).The files are being made available under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).</p
eLife Open Peer Review Corpus
No full texteLife Open Peer Review CorpusSection for Logic & Cognitive Science, Institute of Philosophy and Sociology, Polish Academy of ScienceGenerated by Ksawery Jasieński under supervision of Marcin Miłkowski (2022)eLife is committed to open peer review idea. Reviews are not available for download in a single package, so they must be extracted from their complete data set, which is several gigabytes large.The data set contains all available peer reviews as of 24 July 2022 (10853 papers with reviews or at least decision letters that omit minor comments). As stated by eLife:“In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.”In addition, the corpus contains metadata about particular reviews (the filename contains ‘r’ and a numerical id before ‘.xml’), author responses (the filename then contains ‘a’ and a number before the ‘.xml’ suffix) and paper metadata in the JSON format. The JSON schema files are available in the schema subdirectory in files with self-explanatory names.The original files were not enriched with any linguistic annotation or converted (these are in XML format, as used by eLife).Additionally, we are making the crawler code available, in the review_crawler directory. For eLife reviews, elife_crawler.py should be used (more notes in the subdirectory).The files are being made available under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).</p
Marcin Świetlicki : poeta (nie)religijny
No full textThe subject of this article is the issue of religiousness in the poetry of Marcin Świetlicki. The works by the author of Niskie pobudki (Low Motives) are entangled in a space of tran¬scendence by (among others) utilizing biblical themes — Cain and Abel, the creation of the world, Christmas, Jesus Christ, salvation — which accompany the author of Pieśń profana (The Profaner’s Song) ever since his first book, published in 1992. These result in the impression of religiousness of the poet’s works. However’ most common dogmas of Christian faith (since this is the only faith the poet refers to), as well as notions of the almighty, all-powerful, mer¬ciful God-the-Father or God the Creator are contradicted in this poetry. The author endues everyday, common things with holiness and divinity, while reducing the existence of God only to humans (thereby, to himself as well) and space available to them
Dragon over the Dnieper. China in relations with Belarus and Ukraine after 2010
No full textWe wstępie do niniejszej książki zaprezentowany zostanie aktualny stan badań oraz główne założenia związane z zaangażowaniem Chin w regionie Europy Środkowej i Wschodniej. Ponadto przedstawione zostaną szczegóły na temat warsztatu badawczego (w postaci podejścia teoretycznego, przyjętych hipotez badawczych, wykorzystanego materiału itd.). Rozdział drugi poruszy problematykę relacji ChRL i Republiki Białorusi w obszarze gospodarczym i militarnym (w mniejszej mierze politycznym). Natomiast rozdział trzeci (poświęcony Ukrainie) skupi się na relacjach gospodarczych, jak i politycznych w kontekście „rewolucji godnościowej” czy następującej po niej aneksji Krymu oraz wojnie ukraińsko-rosyjskiej w Donbasie. Ostatnią część pracy stanowić będzie podsumowanie w oparciu o wnioski z wcześniejszych rozdziałów. Ponadto w zakończeniu współpraca z Białorusią i Ukrainą zostanie przeanalizowana również z perspektywy chińskiej. Autor postanowił wyabstrahować to zagadnienie w celu lepszej eksplanacji istoty zaangażowania Państwa Środka nad Dnieprem. Biorąc pod uwagę, iż motywacje decydentów w Pekinie odnośnie współpracy są zbliżone, takie wyodrębnienie ma zdaniem autora logiczne uzasadnienie. Na ostatnich stronach opracowania znajdują się aneksy w postaci zestawienia wymiany handlowej Chin z krajami Europy Środkowej i Wschodniej (wraz z Rosją) oraz mapy przedstawiające przebieg Nowego Jedwabnego Szlaku. Załączniki mają charakter rozszerzający względem informacji zawartych w głównej części opracowania.The preface to this book there will present the current state of research within the author’s area of interest and the main assumptions connected with the China involvement in the region of Central-Eastern Europe. Besides the preface will present the details concerning author’s research workshop (e.g. theoretical approach, main hypothesis and research materials, etc.). The second chapter will raise the issue of the relations between PRC and Belarus within the field of the military, economic and political affairs. The third chapter in turn devoted to Ukraine will be concentrated upon the economic and military relations as well as political one’s in the following context the Ukrainian Revolution of 2014 and its aftermath: annexation of Crimea and War in Donbass. The last part of this work will constitute a summary based on the conclusions from previous chapters. Moreover the summary will include the analysis of the cooperation between Belarus, Ukraine and China from the China perspective. The author has intended to abstract this issue with the intend to explain more clearly the essence of the China involvement on the Dnieper River. Taking into account that the motivations of the decision-makers in Beijing regarding the cooperation with both Ukraine and Belarus are similar, such abstracting has been, in the author’s view, logically justified. The final pages of this work contain the annexes in the form of the tables with details of trade flow between China and Central-Eastern European countries (including Russia) as well as maps presenting New Silk Road trajectory. Those appendixes has subsidiary character for the rest of research paper
Contemporary world in Marcin Maciejowski’s lens: media realism
No full textThe article deals with the works of Marcin Maciejowski in the perspective of intermedia connections of his works with photography, film and painting. The author of the article is particularly interested in tracing the intertextual and intermedia games in the space of culture as well as their informative, cognitive and ludic functions. A separate section of the article is devoted to the description of media realism
MDPI Open Peer Review Corpus 2
No full textMDPI Open Peer Review Corpus 2Section for Logic & Cognitive Science, Institute of Philosophy and Sociology, Polish Academy of ScienceCognitive Metascience LabGenerated by Ksawery Jasieński, with some input from Remigiusz Depta, under supervision of Marcin Miłkowski (2022-2023)---MDPI is committed to open peer review idea, but these are voluntary. They are not available for download in a single package, so they must be crawled from their website.This dataset contains all peer reviews available on mdpi.com as of January 2023, covering over 135 thousand papers. These are in plaintext format (look for TXT files). In addition, the corpus contains metadata in JSON format for particular reviews, author responses, as well as original paper metadata. For reference see the JSON `schema` files available in the GitHub repository associated with this project.Additionally, this dataset contains the source HTML for each website from which the text of reviews was extracted, as well as any supplementary materials attached with the reviews. The original files were not enriched with any linguistic annotation or converted to any format (these are predominantly PDF and DOCX files, as uploaded through the MDPI editorial system by authors and reviewers).We are making source code available for the dedicated crawler that was built to scrape the MDPI database. See the GitHub link below:https://github.com/cognitive-metascience/review_crawler/tree/main/crawlingSee this corpus on PubPeer:https://pubpeer.com/publications/25353AAFD4FC52E2BEC8C7AD08B259#---This dataset is split into parts because of the upload limits of this repository. The archives are available in ZIP (33 parts, look for `.z[01-33]` files) and 7z (23 parts) formats. In addition, we provide the set of excluded articles with incomplete information (e.g., missing some reviews in the first round etc.) in the `mdpi-dump-dir.zip` file.The dataset after unpacking is a little over 170 GB in size.The files are being made available under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Changelogcurrent version - August 2023no new reviews addedre-scraped (June 2023) the reviews that had some sections of text missingdataset now also includes the source HTML from mdpi.com for each reviewed article. The webpage content was cleaned before storing to an HTML file: specifically, all comments are removed from the document, as well as the following tags: 'script', 'style', 'noscript', 'link', 'rect'.14th March 2023first submission of this datasetreviews was scraped in early January 2023dataset contains metadata for 135652 peer-reviewed articles from the MDPI database, along with full plain text for each review and any supplementary materials that were attached (PDF or DOC files containing e.g. author responses to comments)</p
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