6 research outputs found
Review of environmental benefits and development of methodology for EUNIS habitat changes from nature-based solutions: Application to Denmark and the Netherlands
No full textNature-Based solutions (NBS) are the measures supported by natural processes that can adapt to changing climates and generate diverse social, economic, and environmental benefits. Recognising the potential for additional NBS benefits, and quantifying these benefits is essential as it encourages decision-makers to implement and scale-up NBS initiatives. This paper presents findings from a systematic literature review. The review focused on tools and methodologies used for assessing the environmental benefits of implementing NBS. This review provides a detailed compilation of environmental indicators supported by assessment tools. It also includes a catalogue of tools for evaluating environmental benefits, thereby identifying research gaps. Moreover, this research proposes a methodology that uses an ArcGIS (Architecture of Geographic Information Systems) toolbox to identify habitat changes resulting from the implementation of NBS. The methodology translates CORINE (Coordination of Information on the Environment) land cover classes to EUNIS (European Nature Information System) habitat classes. The developed toolbox was applied to two case studies: Denmark (12 NBS) and the Netherlands (3 NBS). The assessment aimed to compare the habitat changes between 2000 and 2018 as two extreme time points for NBS implementation for both case studies. Results indicate that NBS implementation can change habitats leading to an increase in the Red-necked Grebe population in Denmark and a decline in the Black-tailed Godwit population in the Netherlands (two threatened species). The population change highlights the potential positive and potential negative impacts of NBS in their respective cases. These findings suggest Denmark could benefit from lake construction and restoration projects. At the same time, the Netherlands could invest in wetlands and meadows construction and restoration projects to protect the respective species. They could establish designated breeding zones to ensure their population does not decline rapidly.BT/Environmental BiotechnologyHydraulic Structures and Flood Ris
Effect of different substrate sterilization methods on performance of oyster mushroom (Pleurotus ostreatus)
No full textSaabunud / Received 21.01.2021 ; Aktsepteeritud / Accepted 16.04.2021 ; Avaldatud veebis / Published online 16.04.2021 ; Vastutav autor / Corresponding author: Sanju Shrestha [email protected] sterilization of substrates is an indispensable step in oyster mushroom cultivation. Oyster mushroom growers in Nepal usually follow three different substrate sterilization methods; however, their comparative effectiveness is vastly unexplored. Thus, these experiments were carried out at the Institute of Agriculture and Animal Science (IAAS), Lamjung Campus, Lamjung, Nepal from January to March, in the years 2017 and 2019. The objective of these experiments was to identify the most appropriate method of sterilization. Three different types of sterilization methods viz chemical sterilization (formaldehyde + carbendazim), steam sterilization, and hot-water sterilization were evaluated for the growth parameters and productivity of oyster mushroom cultivated on rice straw. The experiments were laid out on Completely Randomized Design (CRD) with ten replications. The results showed that the spawning rate was 3.2% of the wet substrate. Data were collected until the third flush. A significantly longer duration to colonize the substrate (29.7 days) was observed under chemical sterilization. The oyster mushroom performed best under steam sterilization as it took the shortest time for pinhead formation (34.30 days), fruiting body formation (43.60 days), cropping duration (89.30 days), and produced the highest mushroom yield (1401.9 g per 4 kg bag), and consequently, the highest biological efficiency (101.38%). Average pileus diameter and stipe length were statistically indifferent among the treatments suggesting the significant effect of sterilization methods on the yield of oyster mushroom but not on its morphological attributes
Epithelial-to-mesenchymal transition (EMT) and cancer metastasis: the status quo of methods and experimental models 2025
No full textAbstract Epithelial-to-mesenchymal transition (EMT) is a crucial cellular process for embryogenesis, wound healing, and cancer progression. It involves a shift in cell interactions, leading to the detachment of epithelial cells and activation of gene programs promoting a mesenchymal state. EMT plays a significant role in cancer metastasis triggering tumor initiation and stemness, and activates metastatic cascades resulting in resistance to therapy. Moreover, reversal of EMT contributes to the formation of metastatic lesions. Metastasis still needs to be better understood functionally in its major but complex steps of migration, invasion, intravasation, dissemination, which contributes to the establishment of minimal residual disease (MRD), extravasation, and successful seeding and growth of metastatic lesions at microenvironmentally heterogeneous sites. Therefore, the current review article intends to present, and discuss comprehensively, the status quo of experimental models able to investigate EMT and metastasis in vitro and in vivo, for researchers planning to enter the field. We emphasize various methods to understand EMT function and the major steps of metastasis, including diverse migration, invasion and matrix degradation assays, microfluidics, 3D co-culture models, spheroids, organoids, or latest spatial and imaging methods to analyze complex compartments. In vivo models such as the chorionallantoic membrane (CAM) assay, cell line-derived and patient-derived xenografts, syngeneic, genetically modified, and humanized mice, are presented as a promising arsenal of tools to analyze intravasation, site specific metastasis, and treatment response. Furthermore, we give a brief overview on methods detecting dissemination and MRD in carcinomas, highlighting its significance in tracking the course of disease and response to treatment. Enhanced lineage tracking tools, dynamic in vivo imaging, and therapeutically useful in vivo models as powerful preclinical tools may still better reveal functional interdependencies between metastasis and EMT. Future directions are discussed in light of emerging views on the biology, diagnosis, and treatment of EMT and metastasis
Assessing Changes in Exposure and Women's Health among Households Using Different Cooking Fuels in Chitwan, Nepal
No full textOsteopontin : A Key Multifaceted Regulator in Tumor Progression and Immunomodulation
No full textThe tumor microenvironment (TME) is composed of various cellular components such as tumor cells, stromal cells including fibroblasts, adipocytes, mast cells, lymphatic vascular cells and infiltrating immune cells, macrophages, dendritic cells and lymphocytes. The intricate interplay between these cells influences tumor growth, metastasis and therapy failure. Significant advancements in breast cancer therapy have resulted in a substantial decrease in mortality. However, existing cancer treatments frequently result in toxicity and nonspecific side effects. Therefore, improving targeted drug delivery and increasing the efficacy of drugs is crucial for enhancing treatment outcome and reducing the burden of toxicity. In this review, we have provided an overview of how tumor and stroma-derived osteopontin (OPN) plays a key role in regulating the oncogenic potential of various cancers including breast. Next, we dissected the signaling network by which OPN regulates tumor progression through interaction with selective integrins and CD44 receptors. This review addresses the latest advancements in the roles of splice variants of OPN in cancer progression and OPN-mediated tumor-stromal interaction, EMT, CSC enhancement, immunomodulation, metastasis, chemoresistance and metabolic reprogramming, and further suggests that OPN might be a potential therapeutic target and prognostic biomarker for the evolving landscape of cancer management
Molecular Insights on Signaling Cascades in Breast Cancer: A Comprehensive Review
No full textThe complex signaling network within the breast tumor microenvironment is crucial for its growth, metastasis, angiogenesis, therapy escape, stem cell maintenance, and immunomodulation. An array of secretory factors and their receptors activate downstream signaling cascades regulating breast cancer progression and metastasis. Among various signaling pathways, the EGFR, ER, Notch, and Hedgehog signaling pathways have recently been identified as crucial in terms of breast cancer proliferation, survival, differentiation, maintenance of CSCs, and therapy failure. These receptors mediate various downstream signaling pathways such as MAPK, including MEK/ERK signaling pathways that promote common pro-oncogenic signaling, whereas dysregulation of PI3K/Akt, Wnt/β-catenin, and JAK/STAT activates key oncogenic events such as drug resistance, CSC enrichment, and metabolic reprogramming. Additionally, these cascades orchestrate an intricate interplay between stromal cells, immune cells, and tumor cells. Metabolic reprogramming and adaptations contribute to aggressive breast cancer and are unresponsive to therapy. Herein, recent insights into the novel signaling pathways operating within the breast TME that aid in their advancement are emphasized and current developments in practices targeting the breast TME to enhance treatment efficacy are reviewed
