1,338 research outputs found
Il barocco a Roma nell'architettura e nella scultura decorativa
No full textSanta Maria Maggiore, vestibule; Published Torino: C. Crudo, 1911-1913. Physical description: 3 v. illus. (includes plans and photographs) 319 plates. In portfolios; pt.1 Chiese; pt.2 Palazzi; pt.3 Fontane e ville. In Italian and French. Source: University of Toronto Libraries; http://main.library.utoronto.ca/ (accessed 12/22/2007
The Enzymology of Cytosolic Pyrimidine 5'-Nucleotidases: Functional Analysis and Physiopathological Implications.
No full textIn mammals, cellular 5'-nucleotidase (5'-NT) activity (EC 3.1.3.5) encompasses a number of genetically and structurally distinct enzyme forms, either membrane-bound or soluble, mainly cytosolic, that are characterized by broad specificity towards nucleoside 5'-monophosphate substrates differing in base (purine/pyrimidine) and/or sugar (oxy/deoxy-ribose) moieties. In particular, among the cytosolic 5'-NTs active towards pyrimidine nucleotides are cN-III and cdN, ubiquitously distributed in mammalian tissues and treated as a single entity in the early days. cN-III was first linked to a genetic defect, hereditary pyrimidine nucleotidase deficiency, associated to a nonspherocytic hemolytic anemia disorder of still unclear mechanism but metabolically characterized by abnormally high levels of pyrimidine compounds and ribonucleoproteins in erythrocytes, as evidenced by occurrence of basophilic stippling on blood smearings. Since the first review on pyrimidine-specific nucleotidases (Amici, A.; Magni, G., Arch. Biochem. Biophys., 2002, 397(2), 184-190), excellent overviews on the topic appeared in the literature. In the present contribution, the major findings on these two enzymatic proteins, cN-III and cdN, will be described with particular emphasis on the relationships between their structure and function, as well as on their roles in normal and pathological conditions. The catalytic mechanism of both specific hydrolytic and phosphotransferase activities, possessed by both enzymes, will be discussed also in the light of recent solution of both cN-III and cdN three-dimensional structures. This review also focuses on possible therapeutic approaches involving cellular 5'-NTs in detoxifying common antiviral and antineoplastic drugs
La chiesa della Regina Pacis a Ostia nei progetti di Giulio Magni
No full textRicostruzione delle vicende storiche cheportarono al progetto e alla fondazione della chiesa Regina Pacis, opera di Giulio Magni, nell'ambito del progtto urbanistico della Nuova Ostia: l'analisi si conlcude con alcune notazioni sullo stato di conservazion
A one-step procedure for the purification of uridine phosphorylase from Escherichia coli
No full textHuman Erythrocyte Pyrimidine 5'-nucleotidase, PN-I
No full textErythrocyte maturation is accompanied by RNA degradation and release of mononucleotides. Pyrimidine 5'-nucleotidase, PN-I, has been purified and characterized. The molecular and enzymatic properties determined for the enzyme shows a 36-kDa and 5.1 pI monomeric protein with no disulfide bridges and no phosphate content. The activity is dependent on Mg2+, while it is inactivated by heavy metals and by thiol-reactive reagents. PN-I is specific for pyrimidine nucleoside monophosphates, including the anineoplastic agents 5'-AZTMP and 5'-Ara-CMP. PN-I possess phosphotransferase activity able to exchange phosphate between pyrimidine nucleoside monophosphates and pyrimidine nucleosides, including AZT and Ara-Cyd. Amino aicd sequence has been obtained from tryptic and CNBr peptides. PM cDNA sequence, coding for a 286-residue protein, has been retrived from tag database, amplified by PCR, and expressed in Escherichia coli. The recombinant protein was fully active and showed identical properties with respect to PN-I. Substantial identity has been revealed with the partial sequences reported for p36, an alpha-interferon-induced protein. The significance of this identity is discussed. (C) 2002 Elsevier Science
Isolation of yeast nuclei. Evidence of chromatin-associated proteolytic activity.
No full textPurified yeast nuclei contain proteolytic activities which are associated with chromatin. pH optimum is in the range 8.0--8.5. Partial purification reveals the presence of three fractions corresponding to different molecular weights. Boiled chromatin supernatants are able to inhibit proteolytic activity. The inhibition effect of various compounds is also described. The purity of the chromatin preparation seems to rule out artifacts due to contamination
La chiesa della Regina Pacis a Ostia nei progetti di Giulio Magni
No full textIl saggio presenta una approfondita ricerca sul progetto di Giulio Magni per la chiesa di Ostia nell'ambito del piano per la nuova borgata concepito da Giovannoni e dall'AACAR. Sono prese in considerazione anche le condizioni attuali dell'edificio in attesa del restauro
La chiesa della Regina Pacis a Ostia nei progetti di Giulio Magni
No full textIl saggio presenta una approfondita ricerca sul progetto di Giulio Magni per la chiesa di Ostia nell'ambito del piano per la nuova borgata concepito da Giovannoni e dall'AACAR. Sono prese in considerazione anche le condizioni attuali dell'edificio in attesa del restauro
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