3,096 research outputs found
Development of novel bioinformatic pipelines for MinION-based DNA barcoding
No full textDNA-barcoding is the process of taxonomic identification based on the sequence of a marker gene. When complex samples are analysed, we refer in particular to meta-barcoding. Barcoding has traditionally been performed with Sanger sequencing platform. The emergence of second-generation sequencing platforms, mainly represented by Illumina, enabled the high-throughput sequencing of hundreds of samples, and allowed the characterization of complex samples through meta-barcoding experiments. However, fragments sequenced with the Illumina platform are shorter than 600 bp, and this greatly limits taxonomic resolution of closely related species. Moreover, both these platforms suffer of long turnaround time, since they require shipping the samples to a sequencing facility, and complex regulations may hamper the export of material out of the country of origin. More recently, Oxford Nanopore Technologies provided the MinION, a portable and cheap third-generation sequencer, which has the potential of overcoming issues of currently available platforms, thanks to the production of long sequencing reads. However, MinION reads suffer of high error rate, therefore suitable analysis pipelines are needed to overcome this issue. In this thesis I describe the development of bioinformatic pipelines for MinION-based DNA barcoding. Starting from the analysis of single samples, I show how improvements both in sequencing chemistry and in software now allow obtaining consensus sequences directly in the field, with accuracy comparable with Sanger. Conversely, when analysing complex samples, sequencing reads cannot be collapsed for reducing the error rate. However, bioinformatic approaches exploiting increased read length largely compensate the higher error rate, resulting in high correlation between MinION and Illumina up to genus level, and a more marked sensitivity of MinION platform to detect spiked-in indicator species. In conclusion, the results presented in this thesis show that bioinformatic pipelines for the analysis of MinION reads can largely mitigate platform issues, paving the way for this platform to become the gold-standard for barcoding in the near future
La pittura mediterranea di Giovanni De Simone
No full textIl contributo prende in esame per la prima volta la produzione pittorica di Giovanni De Simone in relazione alla sua originale ricerca nel campo della ceramica, suo strumento espressivo privilegiato. Il saggio indaga i nodi cruciali della formazione di De Simone in relazione al vivace milieu culturale palermitano e alla luce dell'influenza esercitata da maestri del XX secolo come Pablo Picasso, Henry Matisse e Marc Chagall.The contribution examines for the first time the pictorial production of Giovanni De Simone in relation to his original research in the field of ceramics, his privileged expressive tool. The essay investigates the crucial points of De Simone's training in relation to the lively cultural milieu of Palermo and the influence exercised by 20th century masters such as Pablo Picasso, Henry Matisse and Marc Chagall
Rozpor ako východisko, láska ako smer u Simone Weilovej (Contradiction as base, Love as direction in writings of Simone Weil)
No full textArticle is explaining contradiction and love, Simone Weil‘s essential terms of hermeneutics of human Being. It introduces close relation of these terms with her understanding of God as well as with her overall concept of religion. Author also mentions Simone Weil‘s inspirations with philosophical and spiritual concepts of the East
Mechanistic insights into CO2 activation via reverse water - Gas shift on metal surfaces
No full textBy
the means of density functional theory calculations, we find
that CO<sub>2</sub> activation via reverse water–gas shift
(r-WGS) follows different elementary steps on different metals (Pt,
Rh, Ni, Cu, Ag, and Pd). We relate these differences to the interactions
between the adsorbed oxygen and the metals, which strongly affect
the dissociation activation energy. In particular, CO<sub>2</sub> dissociation
is favored on metals that present high affinity toward oxygen. As
the O interaction with the metals weakens, CO<sub>2</sub> hydrogenation
becomes more favored at the expenses of the dissociation. We found
that the binding energy of oxygen scales almost linearly with the
difference between the activation energy of the two competing paths,
and therefore this quantity can be used as a simple descriptor to
discriminate which of the two mechanisms is dominant on different
metals. Such findings allow rationalization of the different catalytic
cycles reported in the literature for the r-WGS reaction on metal
surfaces
“I beg you to tell me what has become of Djamila”: The Political Mobilization of Simone de Beauvoir’s Readers During the Boupacha Affair
No full textBy Sophia Millman This is a condensed version of a Masters thesis dedicated to the political mobilization of Simone de Beauvoir’s readers. The citations from the letters were translated from French by the author. *** On June 2, 1960, the French government ordered all copies of the daily Algiers edition of Le Monde seized and destroyed to suppress the publication of Simone de Beauvoir’s article “Pour Djamila Boupacha.” Beauvoir, a self-professed “woman of letters”, not “of action[1]”, and one ..
ANTONIO DE SIMONE, ESSERE E POLITICA. DIALETTICA DELL'UMANO, MIMESIS EDIZIONI, MILANO 2020, pp.. 500, COLLANA FILOSOFIE N. 705, ISBN - 9788857571492
No full textI temi affrontati more philosophico da Antonio
De Simone in Essere e politica. Dialettica dell’umano
e i profili politico-filosofici (Machiavelli,
Rousseau, Vico, Kant, Hegel, Simmel, Benjamin,
Horkheimer, Adorno, Habermas, Honneth,
Rawls, Heller, Arendt, Lefort, Ricoeur, Masullo)
ivi discussi criticamente esprimono il bisogno di
analizzare e valutare il ruolo, la funzione pubblica
e il tenore immanente della contraddizione
prossemica entro e oltre l’ethos del moderno
nelle morfologie assunte dalla filosofia contemporanea
della normatività. Un atto interpretativo
in cui è implicito l’agire politico connesso
dialetticamente alla vita e alla metamorfica
costituzione della soggettività nella faglia disegualitaria
tra individualità e società. Attraverso
l’interrogazione “paradigmatica” dei classici
moderni, le riflessioni svolte nel libro nella forma
di Lezioni problematicizzano la scelta esistenziale
che pervade la relazione contingente
tra agire umano, vita quotidiana, libertà, valori e
norme, nella dialettica della modernità. Ritratti
filosofici della condizione umana, o, meglio, del
destino umano nell’ontologia politica del vivente,
che performano l’umano come permeato
dal “vivere in tensione” tra l’essere e il dover
essere, dove gli attori umani, nel loro agire, tra
colpevolezza e innocenza, disvelano l’oscurità
ultima, tra bene e male, tra ragione e virtù, tra
ethos, conflitto e politica, del “teatro storico”
delle maschere della vita. L’Epilogo (in forma
di Appendice) segue alla persuasione (manifestata
nella penultima Lezione) che le “ragioni
della filosofia” possano ancora affrontare, nelle
condizioni dell’eticità post-tradizionale, una difficile
eredità generazionale, che si oggettiva, tra
teoria critica, filosofia e politica, nel passaggio
ai contemporanei, dai maestri agli allievi, per
la libertà, la comunicazione intersoggettiva e il
riconoscimento interumano, e, dunque, nella
paticità e socialità che intrama, nell’arte del
vivere e del conflitto, l’enigmatica vincolatezza
dell’umano, dell’irriducibile soggettività tra essere e politica,
tra realtà e pensiero
I piccoli maestri
No full textScheda relativa all'opera letteraria "I piccoli maestri" di Luigi Meneghello, pubblicata da Rizzoli, Milano, nel 197
A comparative study of form and theology in the works of Flannery O'Connor and Simone Weil
No full textIn this comparative study of the form and theology of Flannery O'Connor and Simone Weil I interrogate how Weil's philosophical writings and her theology illuminate O'Connor's use of both narrative and non-fictional forms, and her Catholicism. The Introduction analyses how Weil's concept of superposed reading provides a new method of approaching both O'Connor, her writings, and O'Connor
studies, and focuses on how such apparently different women interconnect. Chapter One explores how both Weil and O'Connor attempt to write their theologies on the
souls of their readers yet are each subject to constraints imposed by form. Weil's concept of locating equilibrium between incommensurates is discussed, and her
distinctively philosophical approach to fictions and fictionality is used to investigate O'Connor's notion of prophetic fictions and the writer's role. Chapter Two assesses how both writers revivify Christian paradoxes. Weil's monstrous concept of affiiction, and O'Connor's use of the grotesque genre to jolt secular man into an
awareness of the sacred are scrutinised. Chapter Three studies how both writers consider an encounter between God and man is possible through the action of grace. My Conclusion interrogates how Weil's work can deepen our understanding of O'Connor's writings, and examines how successful O'Connor is at realising a truly
Christian literature. I conclude that despite being a writer of powerful fictions, O'Connor can not be totally successful in her mission as writer-prophet because
ultimately fiction escapes orthodoxy
- …
