188 research outputs found
Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts
There are many reports of an arginine-dependent nitric oxide synthase activity in plants; however, the gene(s) or protein(s) responsible for this activity have yet to be convincingly identified. To measure nitric oxide synthase activity, many studies have relied on a citrulline-based assay that measures the formation of L-citrulline from L-arginine using ion exchange chromatography. In this article, we report that when such assays are used with protein extracts from Arabidopsis, an arginine-dependent activity was observed, but it produced a product other than citrulline. TLC analysis identified the product as argininosuccinate. The reaction was stimulated by fumarate (> 500 microM), implicating the urea cycle enzyme argininosuccinate lyase (EC 4.3.2.1), which reversibly converts arginine and fumarate to argininosuccinate. These results indicate that caution is needed when using standard citrulline-based assays to measure nitric oxide synthase activity in plant extracts, and highlight the importance of verifying the identity of the product as citrulline.Rudolf Tischner, Mary Galli, Yair M. Heimer, Sarah Bielefeld, Mamoru Okamoto, Alyson Mack and Nigel M. Crawfor
The Contribution of Outer HI Disks to the Merging Binary Black Hole Population
Peer reviewe
She Gets the Girl
Alex Blackwood is an undeniably courageous flirt. Molly Parker is a socially awkward compassionate soul. The duo strikes a deal that helps Molly explore her flirtatious nature and helps Alex prove to her ex that she is not self-centered. The question is: Do Molly and Alex want other people or each other? Author Alyson Derrick, a No. 1 New York Times best-selling author, depicts a beautiful dichotomy between wants and needs in romance.https://ecommons.udayton.edu/ul_popularromance/1050/thumbnail.jp
Instrumental and sensory characteristics of selected nutritionally improved school foods
Childhood obesity is a growing problem in the United States. Recently enacted Federal and State regulations require school foods to be improved nutritionally. The objective of this study was to determine quality of selected newly developed school foods using instrumental and sensory methods. From the National School Lunch Program reimbursable meals chicken nuggets, macaroni and cheese, and pierogies were selected. From the Competitive Foods apple snacks were used. Surface color parameters (L, a*, and b*) were determined by colorimeter and digital camera. A texture analyzer (TA-xT2i) was used to measure texture specific to each product. Sensory Quantitative Descriptive analysis was used to evaluate six to eight sensory attributes per product. The results were compared with similar traditional products. Chicken nuggets with whole grain breading were darker red (L 132.43 and a* 147.96) and coarser, while home style were more yellow (b* 188.44) with firmer breading (407.59 g) and original variety was less yellow and red (b* 182.69 and a* 138.01) with softer meat texture (462.26 g/cm). Macaroni and cheese with 26% reduced fat was significantly saltier and less viscous than original (1486.21 g), however not significantly different in color, cheese aroma, or sweetness. Pierogies with 70% more protein per serving were significantly different with a firmer, grainier filling and darker surface color with values of L 59.14 and b* 14.98 and peak force of filling 7968.9 g. Freeze dried enriched apple snacks were significantly lighter in color, more red (L 170.13 and a* 137.71), firmer texture with average 2821.24 g peak force, more sour, bitter and had a rougher surface texture than air-dried apple snacks. Instrumental color, texture, and sensory characteristics of nutritionally improved products differed significantly from the similar traditional products. Additional efforts of processors will be necessary to prepare nutritionally improved products with high children's acceptability.M.S.Includes bibliographical references (p. 94-104)by Alyson Mandevill
The Butch Monologues: Performance as a Bridge from “Border Wars” to “Playground”
In this chapter Alyson Campbell looks at The Butch Monologues (TBM) by Libro Levi (Doc) Bridgeman, directed by JulieMc McNamara (Mack) (2013–present). Based mainly on an interview with the writer and director, and Campbell’s own multiple viewings of the work, the chapter examines how the collection and, more precisely, the productions of it, make an intervention into this very painful contemporary context, and history, of ‘“border wars’ between butch lesbians and trans men” (Mackay in J Lesbian Stud 23:399, 2019b; and see Halberstam, 1998). It is suggested that TBM manage to blur these borders, or at least niggle this negative framing, and Campbell argues that the stories it tells are more relevant than ever, given this current tension
US97: Dover Ln to Bear Dr safety improvements, MP 97.5 to 100.5, transportation management plan
prepared by: Alyson Shubert, EIT, R4 Traffic Designer ; reviewed by: Teresa Gibson, PE, R4 Traffic Analyst.Title from PDF cover (viewed on October 17, 2022)."K22520."This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Mode of access: Internet from the Oregon Government Publications Collection.Text in English
A Thesis in Seasons
A personal take on a thesis. Alyson Davies uses narratives of her rural Albertan upbringing to position her art practice and experience in Emily Carr University’s MFA program. Davies painting, primarily based in autobiographical narratives, is also met by supplementary practices across other media where seasonality and nature direct the subject of the works. The thesis paper aims to contextualize Davies’ artworks along other artist’s work, and within the physical and inner worlds the work exists in.SeasonalityPrairiesFarmingFarmBetty WoodmanVanessa BellMary FeddenAnita Klei
On necrocapitalism: A plague journal
M.I. Asma is the collective designation for six authors from Canada and the United States, representing a variety of revolutionary anticapitalist theoretical persuasions: J. Moufawad-Paul, Devin Zane Shaw, Mateo Andante, Johannah May Black, Alyson Escalante, and D. W. Fairlane. As the pandemic transitioned from science fiction to reality in early 2020, a number of writers and thinkers in the imperialist metropoles declared the impossibility of writing in the face of a future that is foreclosed. And yet, due to the nightmare that capitalism has been since its beginning, numerous writers and thinkers from the margins have always written in the face of such foreclosure. Meanwhile, other contemporary thinkers sought to conceptualize the unfolding pandemic according to conceptions of bio/necropolitics, forgetting the foundation upon which these conceptions have always existed.
The M.I. Asma writing group came together to stake out a different terrain, thinking through the pandemic as events unfolded while also always working to think beyond the capitalist imaginary. Writing between April 2020 and May 2021, the authors set out to produce a serial theoretical philosophical project focused on class struggle in the midst of the COVID-19 pandemic. The authors approached the pandemic as an occasion to think capitalism according to what it always has been, what the pandemic reveals about its current ideological deployment, and how we can think about a communist alternative in the face of exterminism.
This book collects, with some revisions and with a new epilogue, the entries from the On Necrocapitalism blog, where M.I. Asma’s interventions first appeared.DC Author's celebration 202
Utilizing content analysis on TheKnot.com to study an online wedding planning community for New Jersey
As with other aspects of our culture today, wedding planning is increasingly discussed and implemented online. Information is disseminated through online media attention, advertising, and computer mediated communication. The latter provides an avenue through which the impact of community building and relational culture can be established. My dissertation analyzes an online wedding planning forum on TheKnot.com to exemplify the ways by which a community is built through online postings and discusses the characteristics of this community. Utilizing the uses and gratification theory, content gratifications are examined through the types of topics publically viewable on an active online wedding planning Website. Because of the extensive reach of such forums, one particular region, New Jersey, will be examined. Process gratifications will be discussed as participants share personal experiences from their time spent on this particular online bulletin board planning their wedding celebration. Furthermore, the dynamics of interaction among this group will be analyzed over the course of half a calendar year and will show the types of interaction taking place through content analysis.Ph.D.Includes bibliographical referencesby Alyson H. Theli
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