1,724,399 research outputs found
Intervista a Dario Maccarrone
No full textIl contributo riporta gli esiti dell'intervista effettuata a Dario Maccarrone, curata dall'autore dell'articolo, in riferimento al ruolo delle startup nei territori interni
Assay of NAPE-PLD Activity
No full textN-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC)
The account of a lifetime
No full textThe book is a scientific tribute to Adriano Gozzini (1917-1994) on the occasion of the 20th anniversary of his death. Pioneer of molecular spectroscopy of microwave, optical pumping and laser spectroscopy, Gozzini was one of the leaders of the last century in the field of atomic physics.
The book reproduces 20 articles written by Adriano Gozzini, including an unpublished preprint, chosen to provide a complete picture of the different topics addressed by the scientist during his scientific career. Each item is associated commentary by scientists who have collaborated with him or international experts on the subject matter, including Nobel laureates C. Cohen-Tannoudji, T.W. Haensch and S. Haroche
MODULATION OF THE ENZYMATIC ACTIVITY AND MEMBRANE BINDING PROPERTIES OF SOYBEAN LIPOXYGENASE-1 THROUGH LIMITED PROTEOLYSIS AND METAL SUBSTITUTION
No full textLipoxygenases are non-heme, non-sulfur iron containing enzymes that catalyze the dioxygenation of polyunsatured fatty acids containing one or more pentadiene systems to the corresponding hydroperoxy derivatives. Structural studies in solution of the mammalian and plant enzyme revealed that the latter has a more stable and compact conformation1. As yet, metal atom extraction, reconstitution and substitution with vicariate metals have not been successfully applied to soybean lipoxygenase-1, because of the highly buried position of the iron atom within the active site. Tryptic digestion of lipoxygenase-1 and the subsequent isolation of the 60 kDa C-terminal region allowed to generate a “mini-lipoxygenase-1 (miniLOX)” that retains the catalytically active iron, but in a more accessible position2. In this study, we investigated by near-UV-circular dichroism and fluorescence spectroscopies the structural and functional effects of iron removal, reconstitution and vicariation in miniLOX. Moreover, we report the kinetic analysis and the membrane binding ability of the apo- and metal-substituted forms of miniLOX, using fluorescence resonance energy transfer and monolamellar vesicles. Taken together, these data demonstrate an unprecedented structural role of iron, which is involved not only in the catalytic activity but also in the membrane binding ability of lipoxygenase-1.1. Dainese E. Sabatucci A. van Zadelhoff G. Angelucci C. B. Vachette P., Veldink G. Finazzi Agrò A. and Maccarrone M. (2005). J. Mol. Biol. 349, 143-152.2. Maccarrone M. Salucci M. L. van Zadelhoff G. Malatesta F. Veldink, G. Vliegenthart J. F. and Finazzi Agrò A. (2001). Biochemistry 40, 6819-6827.[...
Duchesne e La Curia Romana
No full textMaccarrone Michèle. Duchesne e La Curia Romana. In: Mgr Duchesne et son temps. Rome : École Française de Rome, 1975. pp. 401-494. (Publications de l'École française de Rome, 23
Chapter 1. Quest for Magic Bullets to Solve the Endocannabinoid Puzzle
No full textCannabis (Cannabis sativa and Cannabis indica) is one of the oldest cultivated plant. Its extracts contain >110 (phyto)cannabinoids (pCBs), some of which appear of clear therapeutic interest. Among them, ?9-tetrahydrocannabinol (THC) and cannabidiol (CBD) hold promise to treat several human diseases, both within the central nervous system and at the periphery. Yet, the complexity of cannabis makes it challenging to really exploit a specific active ingredient for a specific therapeutic purpose. This complexity of the plant extracts is mirrored, or even exceeded, by the complexity of the molecular targets that pCBs can find in our body, most of which belong to the so-called "endocannabinoid (eCB) system". Here, I describe the main components of this signalling system, in order to appreciate how challenging it is to develop drugs that can hit specifically each of them, with a benefit for human health
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