3,109,550 research outputs found
α2-6SLN-lipo-PGA inhibited SARS-CoV-2 infection.
No full text(A, B) SARS-CoV-2 or SARS-CoV infection assays were performed in the presence or absence of the indicated compounds. SARS-CoV-2 or SARS-CoV infection was determined by detecting SARS-CoV-2 RNA in the culture supernatant (A) and viral N protein in the cells (B). Scale bar 50 μm. Lopinavir, 16 μM; remdesivir, 10 μM; α2-3SLN-PGA, 10 mg/ml; α2-6SLN-PGA, 10 mg/ml; α2-6SLN-lipo-PGA, 1 mg/ml. (C) Dose-response curve of α2-6SLN-lipo-PGA upon SARS-CoV-2 (red) or SARS-CoV (blue) infection. Secreted viral RNA was quantified upon treatment with α2-6SLN-lipo-PGA at the concentration as shown. (D, E) Anti-SARS-CoV-2 or anti-SARS-CoV activity of N-acetylneuraminic acid (Neu5Ac, the component of α2-6SLN-lipo-PGA) and lactosylsphingosine-PGA (LS-PGA, the similar structure with α2-6SLN-lipo-PGA without having sialic acid). Remdesivir, 10 μM; Neu5Ac, 80 mM; α2-6SLN-lipo-PGA, 20 μM; LS-PGA, 20 μM. (F) Anti-SARS-CoV-2 activity of indicated compounds in human lung epithelial-derived cell line, Calu-3 cells. Remdesivir, 10 μM; α2-6SLN-lipo-PGA, 10 μM.</p
Season 4, Episode 1: Housing Stability and Eviction Mediation with Joanne Lipo Zovic
No full textRuntime: 22:12In this episode we chat with Joanne Lipo Zovic, a professor, attorney, and eviction mediator about housing stability and eviction mediation. Resources: Mediate Milwaukee (https://www.mediatewisconsin.org/); “Evicted” by Matthew Desmond (https://www.evictedbook.com/); Tenant Resource Center (https://www.tenantresourcecenter.org/mediation); Volunteer Opportunities with the Tenant Resource Center (https://www.tenantresourcecenter.org/volunteer_with_trc)Zielke, Sophia; Lipo Zovic, Haley; Lipo Zovic, Joanne. (2022). Season 4, Episode 1: Housing Stability and Eviction Mediation with Joanne Lipo Zovic. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/260977
Lipo-Dipeptide as an Emulsifier: Performance and Possible Mechanism
No full textA lipo-dipeptide
(C13-lysine-arginine, C13-KR) was designed as
a potential emulsifier with good emulsifying
properties under acidic condition. Compared with two traditional emulsifiers
(whey protein isolate and Tween 80), C13-KR emulsion had
the minimum mean size but the highest zeta potential (around +100
mV). Moreover, C13-KR emulsion showed better stability
against environmental stresses, such as high salt concentrations and
high temperature. The C13-KR particles had the fastest
move rate around 400 Hz when it attained an equilibrium state. Furthermore,
C13-KR emulsifier could sharply reduce the interfacial
tension and had the lowest tension value at the oil/water interface.
The interfacial tension of C13-KR emulsifier was only 3.6
mN/m (0.5% w/v). In conclusion, the lipo-dipeptide C13-KR
could be considered as an emulsifier to produce emulsion under acidic
condition
α2-6SLN-lipo-PGA inhibited SARS-CoV-2 attachment.
No full text(A) Schematic representation of the schedule for treating VeroE6/TMPRSS2 cells with compounds and SARS-CoV-2 in time of addition analysis. Black and white boxes indicate the periods with and without treatment, respectively. (B) The antiviral activities of indicated compounds under treatment protocol as shown in (A) are estimated by quantifying the levels of secreted viral RNA at 24 h post-inoculation. Remdesivir, 8 μM; chloroquine, 8 μM; α2-6SLN-lipo-PGA, 20 μM. (C) SARS-CoV-2 attachment/entry was evaluated using SARS-CoV-2 pseudovirus. VeroE6/TMPRSS2 cells were inoculated with SARS-CoV-2 pseudovirus in the presence or absence of compounds, and at 24 h post-inoculation, cells were lysed and assessed for luciferase activity generated by SARS-CoV-2 pseudovirus infection. Heparin, 50 U/ml; α2-6SLN-lipo-PGA, 10 μM. (D) Virus-cell attachment assay. VeroE6/TMPRSS2 cells were incubated with viruses in the presence or absence of the indicated compounds for 5 min at 4°C to allow virus-cell attachment. After extensive washing, cells were lysed and cell-attached viral RNA was quantified. Heparin, 50 U/ml; chroloquine, 100 μM; α2-6SLN-lipo-PGA, 100 μM.</p
Sialylation of campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice
No full text<p>Background:
Guillain-Barré syndrome (GBS) is a post-infectious polyradiculoneuropathy, frequently associated with antecedent Campylobacter jejuni (C. jejuni) infection. The presence of sialic acid on C. jejuni lipo-oligosaccharide (LOS) is considered a risk factor for development of GBS as it crucially determines the structural homology between LOS and gangliosides, explaining the induction of cross-reactive neurotoxic antibodies. Sialylated C. jejuni are recognised by TLR4 and sialoadhesin; however, the functional implications of these interactions in vivo are unknown.</p>
<p>Methodology/Principal Findings:
In this study we investigated the effects of bacterial sialylation on phagocytosis and cytokine secretion by mouse myeloid cells in vitro and in vivo. Using fluorescently labelled GM1a/GD1a ganglioside-mimicking C. jejuni strains and corresponding (Cst-II-mutant) control strains lacking sialic acid, we show that sialylated C. jejuni was more efficiently phagocytosed in vitro by BM-MΦ, but not by BM-DC. In addition, LOS sialylation increased the production of IL-10, IL-6 and IFN-β by both BM-MΦ and BM-DC. Subsequent in vivo experiments revealed that sialylation augmented the deposition of fluorescent bacteria in splenic DC, but not macrophages. In addition, sialylation significantly amplified the production of type I interferons, which was independent of pDC.</p>
<p>Conclusions/Significance:
These results identify novel immune stimulatory effects of C. jejuni sialylation, which may be important in inducing cross-reactive humoral responses that cause GBS</p>
Ma Ma Ma Mad
No full textMa Ma Ma Mad is an autobiographical work, written and performed by Singaporean-Australian theatre maker Merlynn Tong. This production, presented at the Brisbane Powerhouse in December 2015, was a multi-genre work incorporating aspects of Butoh, physical theatre, cabaret and contemporary monologue. More than an experiment in mixed performative forms, however, this particular production was also an exercise in inter-cultural collaboration as well as gender in (and of) performance. Heavily influenced by the creator's experiences growing up in urban Southeast Asia, the director's specialisation in contemporary Australian theatre and experience telling uniquely Australian stories worked to manipulate the form in an endeavour to succinctly speak to local audiences, without pandering to entrenched stereotypes or diluting the underlying Chinese-Singaporean themes. The success of this production was also somewhat of a personal challenge for the creatives, after being told by some of Brisbane's most influential theatre venues and festivals that they would rather not support the work because a) it was a one woman show, and b) it was a one woman show about an Asian woman; and therefore would not sell well. One very influential local producer even said that he already had a one-woman show about an Asian person programmed, so he couldn't possibly program another. Operating in such a biased and out-of-touch artistic environment was seen as an easy challenge for the artists involved, which resulted in a highly successful and critically acclaimed sell-out run of Ma Ma Ma Mad, followed by offers to tour the work nationally and internationally. As such, this production also stands as a practical example of the ingrained and patriarchal structures of the Australian arts scene, and how art can work to break down the very barriers that it has helped to construct through a lack of vision and diversity amongst its leaders
The composition and properties of prepared Lipo-Dox and AP-1 Lipo-Dox.
No full text<p>The composition and properties of prepared Lipo-Dox and AP-1 Lipo-Dox.</p
Calcein release from LMDP-lipo into cells.
No full text<p>DiI-labeled and calcein encapsulated Control-lipo (EPC/DOPE/CHEMS) (a), LMDP-lipo (EPC/DOPE/CHEMS/5 mol% LMDP) (b), LMDP-lipo in the presence of 50 µM DAPT (c), LMDP-lipo in the presence of 0.4 M sucrose (d), LMDP-lipo in the presence of 2.5 mM amiloride (e) and LMDP-lipo in the presence of 5 µg/mL FilipinIII (f) were added to A549 cells and incubated at 37°C for 1 h. The intracellular location of CM-DiI (red) and calcein (green) was observed by CLSM. Red signal indicates liposomes. Scale bars, 10 µm.</p
Rhizobial lipo-oligosaccharides: answers and questions
No full textRhizobium bacteria produce certain lipo-oligosaccharides (modified chitin oligomers) after induction of nodulation (nod) gene transcription by the host plant. The function of the rhizobial nod genes in the biosynthesis of these lipo-oligosaccharides, focusing on their host specific aspects, is discussed. The lipo-oligosaccharides can elicit various responses in the host plants, like the formation of pre-infection threads and nodule meristems. Speculating on their function in plant morphogenesis the question is raised: do the rhizobial lipo-oligosaccharides resemble unknown plant signal molecules?Microbial Biotechnolog
Mycobacterium Survival Strategy Translated to Develop a Lipo-Peptide Based Fusion Inhibitor
No full textThe entry of enveloped viruses requires fusion of viral and host cell membranes. An effective fusion inhibitor aiming at impeding such virus-host cell membrane fusion may emerge as a broad-spectrum antiviral agent to neutralize the infection from an increasing diversity of harmful new viruses. Mycobacterium survives inside the phagosome of the host cells by inhibiting phagosome-lysosome fusion with the help of a coat protein coronin 1. Structural analysis of coronin 1 and other WD40-repeat containing protein suggest that the tryptophan-aspartic acid (WD) sequence is placed at distorted β-meander motif (more exposed) whereas the WD resides in regular β-meander motif in other WD40 proteins. The unique structural feature of coronin 1 was explored to identify a simple lipo-peptide sequence (lipid-WD), which effectively inhibit the membrane fusion by increasing interfacial order and decreasing water penetration, surface potential. The effective fusion inhibitory role of mycobacterium inspired lipo-dipeptide was applied to combat type 1 influenza virus (H1N1) infection as a ‘broad spectrum’ antiviral agent.<br /
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