66 research outputs found
Fig. 2 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response
No full textFig. 2. Phylogenetic tree of SaNES/LIS protein within representative samples of known plant TPSs. The neighbor-joining tree was drawn using MEGA 7.0 (Kumar et al., 2016). Bootstrap values from 1000 replicates were used to assess the robustness of the trees.Published as part of Zhang, Xinhua, Teixeira da Silva, Jaime A., Niu, Meiyun, Zhang, Ting, Liu, Huanfang, Zheng, Feng, Yuan, Yunfei, Li, Yuan, Fang, Lin, Zeng, Songjun & Ma, Guohua, 2021, Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response, pp. 1-12 in Phytochemistry (112610) 183 on page 4, DOI: 10.1016/j.phytochem.2020.112610, http://zenodo.org/record/829192
Preface to the 30th anniversary volume: A brief history of the journal [Online First Articles]
No full textEscherichia coli (Migula, 1895) Castellani & Chalmers
No full text<i>5.5. Expression of recombination SaNES/LIS in E. coli and purification</i> <p> For heterologous expression, the <i>SaNES/LIS</i> cDNA fragments were amplified using high-fidelity DNA polymerase PrimerStar (Takara Bio Inc.) and cloned into the pET-28a vector at <i>Bam</i> HI and <i>Xho</i> I sites using specific primers (Table 2). The infusion constructs were verified by DNA sequencing at BGI. The recombinant plasmids were then transformed into the <i>E. coli</i> Rossetta 2 (DE3) strain. A single positive colony was incubated overnight at 37 ◦ C and shaken at 200 rpm in Luria-Bertani (LB) liquid medium with 34 μg/ml chloramphenicol and 100 μg/ml kanamycin. Overnight cultures were diluted 1: 100 with LB supplemented with the same antibiotics as described above until an OD 600 between 0.6 and 0.8 was achieved. IPTG was then added to a final concentration of 0.2 mM and the culture was incubated for an additional 24 h at 16 ◦ C and 150 rpm. Cells were harvested by centrifugation at 7000 <i>g</i> and 4 ◦ C for 5 min, and pellets were resuspended in lysis buffer (10 mM imidazole, 300 mM NaCl, 50 mM NaH 2 PO 4, pH 8.0) containing 1 mM PMSF and 0.5 mg /ml lysozyme. The incubated pellets were disrupted by sonication for 30 cycles (pulse on for 5 s, pulse off for 5 s, 30% amplitude) and the lysate was centrifuged at 12,000 rpm for 30 min. Recombinant His-tagged protein was purified by loading the lysate onto Ni-NTA Hispur Resin according to the manufacturer’ s protocol. The eluted proteins were further desalted on a PD-10 desalting column following the method by Jones et al. (2011). Protein concentration was determined by the Bradford (1976) method. SDS-polyacrylamide gel electrophoresis and Coomassie blue staining was used to confirm the presence of purified recombinant proteins.</p>Published as part of <i>Zhang, Xinhua, Teixeira da Silva, Jaime A., Niu, Meiyun, Zhang, Ting, Liu, Huanfang, Zheng, Feng, Yuan, Yunfei, Li, Yuan, Fang, Lin, Zeng, Songjun & Ma, Guohua, 2021, Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol / linalool synthase gene involved in stress response, pp. 1-12 in Phytochemistry (112610) (112610) 183</i> on page 10, DOI: 10.1016/j.phytochem.2020.112610, <a href="http://zenodo.org/record/8291922">http://zenodo.org/record/8291922</a>
Santalum album
No full text<i>3.1. Identification of a bifunctional nerolidol/linalool synthase in S. album</i> <p> It is well known that essential oils extracted from the heartwood of sandalwood have important economic value. More than 100 terpenoids have been identified from the essential oil of sandalwood heartwood, comprising four main sesquiterpenols, α-, β-, and <i>epi</i> -β- santalol and α- <i>exo</i> -bergamotol, and a large number of minor components, such as bisabolol, (<i>E</i>)-nuciferol, and nerolidol (Baldovini et al., 2011). Several TPSs in <i>S. album</i>, including SaSSY, SamonoTPS1, SasesquiTPS1, SaSQS1, SaSQS2, SaBS, and SaTPS1 to SaTPS3, have been functionally characterized (Jones et al., 2008, 2011; Srivastava et al., 2015; Zhang et al., 2019). Additionally, a few TPSs in other <i>Santalum</i> species, such as SauSSY, SspiSSY, SauBS, SspiBS, and SspiTPS4, have been reported (Jones et al., 2011; Moniodis et al., 2015). The majority of these TPSs contribute to aromatic essential oil in heartwood. SaTPS1, which mainly produced α- terpineol, (<i>E</i>)-α- bergamotene and sesquisabinene, and SaTPS3, which mainly produced (<i>E</i>)-β- farnesene, (<i>E</i>)-nerolidol and (<i>E, E</i>)-farnesol, were involved in the defensive response of <i>S. album</i> to biotic attack and abiotic stresses (Zhang et al., 2019). In the present study, one novel TPS was selected from the unigenes of transcriptome data from <i>S. album</i> leaves in response to cold stress and further functionally characterized. Recombinant SaNES/LIS generates (<i>E</i>)-nerolidol and linalool utilizing FPP and GPP as substrates, respectively. Transient expression assays demonstrated that SaNES/LIS produced the same product <i>in planta</i> as that obtained <i>in vitro</i>. Combined with the expression pattern of the <i>SaNES/LIS</i> gene, <i>SaNES/LIS</i> might play another role that does not contribute to essential oils in sandalwood species.</p>Published as part of <i>Zhang, Xinhua, Teixeira da Silva, Jaime A., Niu, Meiyun, Zhang, Ting, Liu, Huanfang, Zheng, Feng, Yuan, Yunfei, Li, Yuan, Fang, Lin, Zeng, Songjun & Ma, Guohua, 2021, Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol / linalool synthase gene involved in stress response, pp. 1-12 in Phytochemistry (112610) (112610) 183</i> on page 5, DOI: 10.1016/j.phytochem.2020.112610, <a href="http://zenodo.org/record/8291922">http://zenodo.org/record/8291922</a>
FINE: A Framework for Distributed Learning on Incomplete Observations for Heterogeneous Crowdsensing Networks
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The effect of mechanical parameters of switch-type contact on relay operation characteristics
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Upconversion luminescence and magnetic properties of ligand-free monodisperse lanthanide doped BaGdF5 nanocrystals
No full textMangrove Soil-Borne Trace Elements in Qi’ao Island: Implications for Understanding Terrestrial Input of Trace Elements into Part of the Pearl River Estuary
No full textAn investigation was conducted to characterize the trace element status of mangrove soils of Qi’ao Island in the Pearl River estuary. The results show that the spatial variation in the soil-borne trace elements in the investigated area was minor and most of the trace elements were at a level higher than those in other mangrove wetlands around the world, suggesting the mangrove soils of Qi’ao Island were heavily contaminated by trace elements transported from the Pearl River in the past two decades. Zn was closely related to Pb, Cu, Cd, and As, while some trace elements were not closely related to each other, indicating that they were derived from different sources. An integrated Nemerow pollution index of the surface soils at the 17 sampling locations ranged from 7.53 to 48.42, values which all fall within the highest pollution category. Among the 17 sampling locations, six locations had an ecological risk index (ERI) greater than 300, and 12 locations had an Ecological Risk Index (ERI) greater than 600, indicating that most of the investigated locations were at high or very high ecological risk. The findings obtained from this study have implications for understanding the terrestrial inputs of trace elements into part of the Pearl River estuary. This understanding can be used to guide the development of management strategies for controlling the discharges of trace elements from the catchment area and managing the aquatic ecosystems in the Pearl River Estuary
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