23,733 research outputs found
Using RT to detect brand specific attributes
No full textQuestionnaires are often used in exploring the brand specific attributes (BSA) in people’s mind. In this study, subjects were asked to judge whether the word presented is associated with the brand or not, meanwhile their response time (RT) was recorded. The result shows that RT measurement might be efficient to detect BSA. Therefore, it’s worthy to explore the criteria for the level of RT which distinguishing BSA from common brand association. Correspondingly, the other experiment held in real market showed RT measurement could be a good supplement to the national questionnaire investigation to disclose the important brand attributes
RT-qPCR validation.
No full textEF1a was used as the internal control. The error bars represent the SE of the RT-qPCR data (n = 3). “r” represents the Pearson correlation coefficient. Pearson correlation between the RNA-Seq data and RT-qPCR data was calculated using the log2 fold change and the relative expression level.</p
Characterization of MERS-CoV-MA-Δ4b and MERS-CoV-MA-mNLS mutants.
No full text(A) Huh-7 cells were infected with MERS-CoV-MA, MERS-CoV-MA-Δ4b or MERS-CoV-MA-mNLS at a m.o.i. of 0.1 for 24 h and the localization of 4b (in green) was analyzed by confocal microscopy. (B) Subconfluent monolayers of Huh-7 were infected with wild-type (black), Δ4b (red) or mNLS (purple) at a m.o.i. of 0.1 or 0.001. Culture supernatants were collected at 24, 48, 72 and 96 h p.i. and titrated by plaque assay. The average of two independent experiments is represented. (C) Pro-inflammatory response induced in Huh-7 by the infection with WT, Δ4b or mNLS. RNA was collected at 24 h p.i and quantified by RT-qPCR. Error bars represent standard deviations of the mean (n = 3). Differences with WT group were analyzed by Student’s t test: *, p-value < 0.05; **, p-value < 0.01.</p
Innate immune response and autophagy flux in MERS-CoV-MA-WT and MERS-CoV-MA-Δ4b infected MRC-5 cells after the autophagy pathway was chemically inhibited.
No full textmRNA expression levels of genes related to autophagy pathway and pro-inflammatory response were quantified by RT-qPCR in MRC-5 cells either mock-infected or infected with MERS-CoV-MA-WT or MERS-CoV-MA-Δ4b at a m.o.i of 1 for 24 h p.i after the treatment (+ 3-MA) or not (- 3-MA) with 5mM 3-methyladenine for 16 h. Bars represent means and error bars represent standard deviations (n = 3). Mock, Mock-infected cells. Differences were analyzed by Student’s t test: *, p-value < 0.05; **, p-value < 0.01; ***, p-value < 0.001. Asterisks on the columns represent significant differences between mock-treated and cells.</p
Innate immune response to MERS-CoV-MA-Δ4b in mice.
No full texthDPP4-KI mice were infected with 5x104 PFU of MERS-CoV-MA-WT, MERS-CoV-MA-Δ4b or MERS-CoV-MA-mNLS virus. Mice were sacrificed at 4 and 6 dpi and the RNA of the lungs was extracted for gene expression assays. mRNA expression levels of genes related to the IFN response (A), the pro-inflammatory response (B), and autophagy (C) were quantified by RT-qPCR. Error bars represent standard deviations of the mean (n = 3). Differences between groups were analyzed by Student’s t test: *, p-value < 0.1; **, p-value < 0.01.</p
Encouraging Treatment Concordance in Type 2 Diabetes
No full textThe purpose of this article is to reflect on the importance of education and patient involvement in the prescribing process in diabetes management. This article presents a case study of a patient with type 2 diabetes mellitus referred to the diabetes specialist nursing service due to long-standing poor glycaemic management and reflects on clinical inertia and medicines
optimisation. Using a case study from clinical practice, insights and recommendations can be made. This process enables knowledge transferability to be applied in diabetes consultations, affecting medicines optimisation and resistance of clinical inertia in the management of type 2 diabetes. Clinicians must strive to ensure patient involvement in the prescribing process. This case study supports the importance of conducting patient-centred medication reviews that support the patient use their medicines. The importance of education regarding the treatments prescribed should not be underestimated and can impact on treatment concordance
Is Death Still a Taboo Subject in Palliative Care?
No full textThe article examines whether death is still a taboo subject in palliative care. Evidence shows that communicating openly about death and dying makes it more convenient to come to terms with. It is said that community nurses still support patients to reframe the life before them. Patients are encouraged to plan in advance through advanced care planning for their preferences around care and death
Minori sulla carta, adulti nella vita: leggere i bisogni e le competenze dei MSNA
No full textNell’attuale quadro dei processi migratori, i “Minori Stranieri Non Accompagnati”, (comunemente noti con l’acronimo “MSNA”), hanno assunto nel corso degli ultimi anni una rilevanza crescente all’interno dei flussi in arrivo in Italia, non solo dal punto di vista quantitativo, ma anche rispetto alle sfide che hanno posto al sistema di accoglienza. Uno dei nodi critici particolarmente diffuso nel sistema di accoglienza è la gestione dei tempi del rilascio dei documenti e dell’avvio di attività di inserimento sociale. Una ulteriore criticità è quella relativa all’offerta di percorsi socioeducativi. La constatazione delle criticità – unitamente alle condizioni di fragilità che la scelta migratoria, prima, e l’arrivo, dopo, inevitabilmente comportano – rende evidente quanto i MSNA necessitino di adeguate misure di accompagnamento, protezione e accoglienza, al fine di creare le condizioni idonee alla realizzazione di percorsi di accoglienza efficaci e capaci di includere in una prospettiva di reciprocità e allo stesso tempo arginare il fenomeno degli allontanamenti volontari
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