170,105 research outputs found
FIGURE 6. Muscari tenuiflorum a in Taxonomic resurrection of Muscari wallii (Asparagaceae, Scilloideae)
No full textFIGURE 6. Muscari tenuiflorum a: habit; b: inflorescence; c: close up of sterile flowers; d: close up of fertile flowers (İ.Eker 2207, 5293, 12479) (photographed by İ.Eker).Published as part of Eker, İsma İl, 2021, Taxonomic resurrection of Muscari wallii (Asparagaceae, Scilloideae), pp. 226-242 in Phytotaxa 513 (3) on page 236, DOI: 10.11646/phytotaxa.513.3.3, http://zenodo.org/record/531318
FIGURE 5. Muscari babachii a in Taxonomic resurrection of Muscari wallii (Asparagaceae, Scilloideae)
No full textFIGURE 5. Muscari babachii a: habit; b–c: inflorescence; d: close up of sterile flowers; e: close up of intermediate fertile flowers (İ.Eker 2189, 2355, 2519) (photographed by İ.Eker).Published as part of Eker, İsma İl, 2021, Taxonomic resurrection of Muscari wallii (Asparagaceae, Scilloideae), pp. 226-242 in Phytotaxa 513 (3) on page 235, DOI: 10.11646/phytotaxa.513.3.3, http://zenodo.org/record/531318
STUDIO DEI MECCANISMI ENDOGENI DI PROTEZIONE DEL MIOCARDIO ISCHEMICO PER L'IDENTIFICAZIONE E IL MIGLIORAMENTO DI STRATEGIE TERAPEUTICHE
No full textIl tessuto cardiaco, quando viene sottoposto ad un insulto ischemico, e' in grado di attivare dei meccanismi di compenso che gli consentono di contrastare, quantomeno temporaneamente, l'insorgenza di lesioni che possono avere come esito finale anche la morte cellulare.
L'obiettivo della presente ricerca e' quello di identificare e caratterizzare alcuni tra i principali processi che proteggono il miocardio durante l'ischemia, al fine di suggerire strategie terapeutiche che ripercorrano i meccanismi biochimici e molecolari spontaneamente selezionati dalla natura. Saranno trattati i seguenti argomenti:
1. Adattamento energetico mitocondriale.
2. Coinvolgimento dei canali mitocondriali del potassio sensibili all'ATP.
3. Fattori responsabili del danno da radicali liberi dell'ossigeno.
4. Meccanismi di apoptosi e necrosi cellulare.
5. Proteine cinasi coinvolte nei processi di adattamento all'ischemia.
6. Effetti del sovraccarico di calcio nelle cellule endoteliali.
7. Stimolazione dei processi di neoangiogenesi.
Nella prima fase del progetto saranno studiati i principali meccanismi di adattamento cellulare in risposta a differenti condizioni di ischemia e riperfusione, mentre nella seconda saranno affrontate le stesse tematiche in riferimento alla protezione indotta da vari tipi di precondizionamento. Saranno impiegati modelli di ishemia/riperfusione e di precondizionamento nel cuore di animali di grossa taglia, nel cuore e nell'aorta isolata di ratto, in cellule cardiache e endoteliali in coltura, nonche' in modelli dove la protezione viene indotta dall'esercizio fisico e dall'iperbarismo
Isolamento e caratterizzazione di cellule progenitrici endoteliali da sangue e midollo osseo di maiale
No full textA cytogenetic analysis of the chromosomes in two related species of the genus <i>Muscari</i> (Liliaceae)
No full text Muscari comosum L. and Muscari matritensis Ruiz Rejón et al. are two closely related species of the subgenus Leopoldia, belonging to the genus Muscari (Liliaceae). Cytogenetic analyses have been made to analyse the differences between these species. Major differences are that M. comosum has four or five dark intercalary 4,6-diamidino-2-phenylindole (DAPI) positive C-bands in the first chromosome pair, whereas M. matritensis has only three thin bands. Muscari comosum has a large chromomycin A3 positive C-band in the fifth, nucleolus organizing region (NOR) bearing chromosome pair, whereas M. matritensis has the CMA3-positive band and the NOR in the short arm of the second pair. The possible role played by occurrences of translocations and amplifications in the chromosomal evolution of these species is discussed.Key words: Muscari, Liliaceae, karyotypes, evolution. </jats:p
Inhibition of the expression of ornithine decarboxylase and c-Myc by cell-permeant ceramide in difluoromethylornithine-resistant leukaemia cells.
No full textCeramide has emerged as a novel lipid mediator in cell growth and apoptosis. In difluoromethylornithine-resistant L1210 cells stimulated to growth from quiescence, the cell-permeant analogues of ceramide N-acetylsphingosine (C2-ceramide) and N-hexanoylsphingosine (C6-ceramide) inhibited the induction of ornithine decarboxylase (ODC) activity with IC50 of 8.3 and 1.5 microM respectively. This effect was strictly related to the ability to inhibit cell growth and [3H]thymidine incorporation. The suppression of cell growth was also associated with apoptosis. The addition of bacterial sphingomyelinase resulted in a significant, but limited, reduction of ODC induction and [3H]thymidine incorporation. Bacterial lipopolysaccharide, which may act as a ceramide analogue, also inhibited the induction of the enzyme. Moreover, C6-ceramide largely prevented the accumulation of ODC mRNA and its precursor, ODC heterogeneous nuclear RNA, that accompanied the induction of ODC activity. A slight increase in ODC turnover was also observed. The DNA-binding activity of some transcription factors known to bind and transactivate the ODC gene was investigated by gel mobility-shift assay under the same experimental conditions. However, only the binding of Myc/Max was negatively affected by the treatment with C6-ceramide. Furthermore, the amount of immunoreactive c-Myc, which increased after stimulation of the cells to growth, was strongly reduced by C6-ceramide. These results suggest that the inhibition of c-Myc and ODC expression may be early events in the response of leukaemia cells to ceramide.IF 5.01
Muscari neglectum GUSS.
No full text<i>Muscari neglectum</i> GUSS. <p>Oberösterreich: Nördliche Kalkalpen, Windischgarstner Becken, Rossleithen, magere Streuobstwiese am S-Abfall des Giererkogels bei Rading, ca. 625m, 8251/4, 24.05.2006, vid. OS.</p> <p> <b>Neu für Oberösterreich</b>. Noch bei FISCHER et al. (2005) wird ein Vorkommen dieser Art in Oberösterreich als fraglich behandelt, in der Checkliste von STRAUCH (1997) fehlt <i>Muscari neglectum</i> generell. Durch den obigen Fund ist die Art somit sicher in diesem Bundesland nachgewiesen, wenngleich der Status des nur durch wenige Individuen gekennzeichneten Vorkommens (ob verwildert oder autochthon) nicht klar ist.</p>Published as part of <i>Stöhr, O., Pilsl, P., Essl, F., Hohla, M. & Schröck, C., 2007, Beiträge zur Flora von Österreich, II, pp. 155-292 in Linzer biologische Beiträge 39 (1)</i> on page 226, DOI: <a href="http://zenodo.org/record/5407540">10.5281/zenodo.5407540</a>
The complexity of platlet metabolism and its contribution to atherotrombosis
No full textActa Cardiol. 2009 Apr;64(2):157-65.
The complexity of platelet metabolism and its contribution to atherothrombosis.
Puddu P, Muscari A, Puddu GM, Cravero E, Giannoni C, Zoli M.
Department of Internal Medicine, Ageing and Nephrological Diseases, University of Bologna and S. Orsola-Malpighi Hospital, Bologna, Italy.
Platelet functions are multiple, complex and not limited to haemostasis. In fact, platelets play a relevant role in vascular inflammation and atherosclerosis (ATS). In the presence of vascular lesions or inflammation, endothelial denudation or activation triggers mechanisms that render the circulating platelets adhesive for the vascular wall. Endothelial lesions expose subendothelial matrix components, such as collagen, von Willebrand factor, fibronectin and other adhesive proteins. Platelet adhesion depends on the interaction between these components and platelet receptors (mainly glycoprotein (GP) VI and GPlb-IX-V). Adhesion triggers the platelet release of inflammatory and mitogenic substances that alter the thromboresistant endothelial surface, enhance the chemoattraction of leukocytes, stimulate smooth muscle cell proliferation and contribute to matrix degradation. Finally, GPIIb-IIIa receptors are activated, leading to firm platelet aggregation and thrombus formation. Platelets participate in the formation of mural thrombi in the late stages of atherosclerotic disease, but also adhere to endothelial cells during the earlier stages of atherosclerotic plaque development. Moreover, platelets exert important functions in modulating inflammatory and immune processes. An improved comprehension of the complex platelet pathophysiology could suggest new therapeutic strategies to reduce the impact of atherosclerotic disease.
PMID: 19476106 [PubMed - indexed for MEDLINE
FIGURE 2. Investigated Muscari species. a in Taxonomic resurrection of Muscari wallii (Asparagaceae, Scilloideae)
No full textFIGURE 2. Investigated Muscari species. a: inflorescence of M. babachii (İ.Eker 2519); b: inflorescence of M. wallii (İ.Eker 12986); c: inflorescence of M. tenuiflorum (İ.Eker 5293); d–e: lateral and longitudinal views of the M. wallii flowers-the two on the left intermediate flowers, the others matured fertile flowers; f: lateral view of the matured M. babachii flower (photographed by İ.Eker).Published as part of Eker, İsma İl, 2021, Taxonomic resurrection of Muscari wallii (Asparagaceae, Scilloideae), pp. 226-242 in Phytotaxa 513 (3) on page 232, DOI: 10.11646/phytotaxa.513.3.3, http://zenodo.org/record/531318
Effect of ischemia on heart submitochondrial superoxide production
No full textNADH-dependent formation of superoxide anions (O2-) by rabbit cardiac submitochondrial particles (SMP) was stimulated after exposure of the isolated heart to 90 min of ischemic perfusion. This effect was more evident in the rotenone-inhibited region of the respiratory electron chain in comparison to the antimycin-inhibited region. The kinetic study of the NADH-dependent reaction showed that at the level of the rotenone-inhibited region, ischemia reduced Km value for NADH, differently from the antimycin-inhibited region where the kinetic constants remain unchanged. No significant changes of the Vmax values were observed in both SMP-producing O2- sites. The ischemic perfusions also produced a reduction of mitochondrial function, particularly evident when glutamate as substrate was studied
- …
