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Oligogalacturonides: long-standing plant signals with a novel signalling role in rhizobium-legume communications
No full textOligogalacturonides: novel signalling molecules in Rhizobium-legume communications
Full text linkOligogalacturonides are pectic fragments of the plant cell wall, whose signaling role has been described thus far during plant development and plant-pathogen interactions. In the present work, we evaluated the potential involvement of oligogalacturonides in the molecular communications between legumes and rhizobia during the establishment of nitrogen-fixing symbiosis. Oligogalacturonides with a degree of polymerization of 10 to 15 were found to trigger a rapid intracellular production of reactive oxygen species in Rhizobium leguminosarum bv. viciae 3841. Accumulation of H2O2, detected by both 2′,7′-dichlorodihydrofluorescein diacetate– based fluorescence and electron-dense deposits of cerium perhydroxides, was transient and did not affect bacterial cell viability, due to the prompt activation of the katG gene encoding a catalase. Calcium measurements carried out in R. leguminosarum transformed with the bioluminescent Ca2+ reporter aequorin demonstrated the induction of a rapid and remarkable intracellular Ca2+ increase in response to oligogalacturonides. When applied jointly with naringenin, oligogalacturonides effectively inhibited flavonoid-induced nod gene expression, indicating an antagonistic interplay between oligogalacturonides and inducing flavonoids in the early stages of plant root colonization. The above data suggest a novel role for oligogalacturonides as signaling molecules released in the rhizosphere in the initial rhizobium–legume interaction
Calcium signals in the early steps of nitrogen-fixing symbiosis: information processing by rhizobia
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Oligogalatturonidi: nuove molecole segnale coinvolte nelle comunicazioni tra batteri azotofissatori e leguminose
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Measurement of cytosolic calcium transients and oxidative burst in aequorin-transformed soybean cells in response to oligogalacturonides
No full textCalcium-mediated communications between rhizobia and plants in the establishment of nitrogen-fixing symbiosis
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