17 research outputs found
Sporadic dyschromatosis universalis hereditaria: A rare case report
No full textDyschromatosis universalis hereditaria (DUH) is a rare genodermatoses characterized by hyperpigmented and hypopigmented macules inherited most commonly in autosomal dominant manner. We hereby report a case of DUH in an adolescent male with palmo-plantar involvement and no family history of the disorder
Author Correction: Biocementation mediated by native microbes from Brahmaputra riverbank for mitigation of soil erodibility
No full textThe original version of this Article contained an error in Figure 4, where Figure 4d was a duplication of Figure 4e. The original Figure 4 and accompanying legend appear below. In addition, in Table 1, in the column “Properties”, “Clay content % (≤ 0.075 mm)”. now reads: “Clay content % (≤ 0.002 mm)”. The original Article has been corrected
A 5-Strand Hamstring Autograft Achieves Outcomes Comparable to Those of a 4-Strand Hamstring Autograft With a Graft Diameter of 8 mm or More in Anterior Cruciate Ligament Reconstruction
No full textBackground: Quadrupled (4-strand) hamstring tendon autografts are commonly used in anterior cruciate ligament (ACL) reconstruction, but there is significant variability in their diameter. The 5-strand hamstring autograft has been used as a means of increasing the graft diameter in patients with undersized hamstring grafts. Purpose: To report the outcomes of primary ACL reconstruction using 5-strand hamstring autografts in patients in whom the 4-strand configuration produced a graft diameter of <8 mm and to compare these outcomes with those of ACL reconstruction using 4-strand semitendinosus-gracilis autografts with a graft diameter of ≥8 mm. Study Design: Cohort study; Level of evidence, 2. Methods: The primary study group comprised 25 patients who underwent ACL reconstruction using a 5-strand hamstring autograft. The comparison group comprised 20 patients who underwent ACL reconstruction using a 4-strand hamstring autograft with a graft diameter of ≥8 mm. Interference screw fixation was used at the tibial and femoral ends for both groups of patients. Subjective questionnaires, including the Knee injury and Osteoarthritis Outcome Score (KOOS), the Lysholm score, and the Physical Component Summary and Mental Component Summary of the Short Form–36 (SF-36), were administered preoperatively as well as at 1- and 2-year follow-up visits. Results: There were no significant differences in the patient demographics and preoperative scores between the 2 groups. The mean graft diameter was 9.06 ± 0.60 mm in the 5-strand group and 8.13 ± 0.32 mm in the 4-strand group ( P < .05). There was no statistically significant difference between groups on postoperative Lysholm, KOOS Pain, KOOS Symptoms, KOOS Activities of Daily Living, KOOS Sports, KOOS Quality of Life, and SF-36 Physical Component Summary scores. Conclusion: In primary ACL reconstruction, the 5-strand hamstring autograft achieves clinical outcomes that are comparable to those of the 4-strand hamstring autograft with a graft diameter of ≥8 mm. The 5-strand graft technique is therefore a useful means of increasing the graft diameter when faced with an undersized hamstring graft. </jats:sec
Five-Strand Hamstring Autograft in Primary Anterior Cruciate Ligament Reconstruction
No full textAbstractThe purpose of this study was to describe our surgical technique of using five-strand hamstring autograft with interference screw fixation in primary anterior cruciate ligament (ACL) reconstruction and to report the early postoperative outcomes of this technique. Patients who underwent primary ACL reconstruction using five-strand hamstring autografts with interference screw fixation between December 2014 and June 2016 were included in this study. The five-strand configuration was used in these patients because the four-strand configuration produced a graft diameter of less than 8 mm. Subjective questionnaires, including the Knee Injury and Osteoarthritis Outcome Score (KOOS) and the Lysholm Score, were administered preoperatively, as well as at 1- and 2-year follow-ups. Paired t-test was used to compare the pre- and postoperative outcome scores. The study group comprised 25 patients. There were no intraoperative complications. The mean follow-up period was 17.8 months (12–24). There were 19 males and 6 females. The median age was 24 years (16–41), and median body mass index was 23.9 (18.5–30.2). The median diameter of the five-strand graft was 9 mm (8–10 mm), with a mean of 9.06 ± 0.60 mm. This was associated with a median graft length of 90 mm (80–100 mm). The postoperative Lysholm, KOOS symptoms, KOOS Pain, KOOS daily function, KOOS sports function, KOOS quality of life, and Short Form-36 Physical Component Summary scores improved significantly compared with the preoperative scores. The use of the five-strand hamstring graft with interference screw fixation in primary ACL reconstruction is associated with significant improvements in patient-reported outcomes in the early postoperative period. The five-strand graft technique is a useful means of increasing graft diameter when faced with an undersized hamstring graft.</jats:p
Co-expression of Arabidopsis NHX1 and bar Improves the Tolerance to Salinity, Oxidative Stress, and Herbicide in Transgenic Mungbean
No full textMungbean is an important pulse crop extensively cultivated in Southeast Asia for supply of easily digestible protein. Salinity severely limits the growth and productivity of mungbean, and weeding poses nutritional and disease constraints to mungbean cultivation. To pyramid both salt tolerance and protection against herbicide in mungbean, the AtNHX1 encoding tonoplast Na+/H+ antiporter from Arabidopsis, and bar gene associated with herbicide resistance were co-expressed through Agrobacterium-mediated transformation. Stress inducible expression of AtNHX1 significantly improved tolerance under salt stress to ionic, osmotic, and oxidative stresses in transgenic mungbean plants compared to the wild type (WT) plants, whereas constitutive expression of bar provided resistance to herbicide. Compared to WT, transgenic mungbean plants grew better with higher plant height, foliage, dry mass and seed yield under high salt stress (200 mM NaCl) in the greenhouse. The improved performance of transgenic plants under salt stress was associated with enhanced sequestration of Na+ in roots by vacuolar Na+/H+ antiporter and limited transport of toxic Na+ to shoots, possibly by restricting Na+ influx into shoots. Transgenic plants showed better intracellular ion homeostasis, osmoregulation, reduced cell membrane damage, improved photosynthetic capacity, and transpiration rate as compared to WT when subjected to salt stress. Reduction in hydrogen peroxide and oxygen radical production indicated enhanced protection of transgenic plants to both salt- and methyl vialogen (MV)-induced oxidative stress. This study laid a firm foundation for improving mungbean yield in saline lands in Southeast Asia
Expression analysis of <i>VrNHX1</i> in early and mid stage mungbean seedlings under various abiotic stresses.
No full text<p>(A) Semi-quantitative RT-PCR for studying expression patterns of <i>VrNHX1</i> under salt stress was performed. Total RNA was isolated from leaves and roots of early (5 days) and mid stage mungbean seedlings (10 days) under 200 mM NaCl treatment at time intervals of 0, 6, 12, 18, 24, and 48 hrs. (B) Semi-quantitative RT-PCR for studying expression patterns of <i>VrNHX1</i> under different abiotic stress conditions such as salt, cold and dehydration stress was studied. Total RNA was isolated from mid stage mungbean seedlings under (A) 200 mM NaCl, (B) Cold (4°C), and (C) 200 mM Mannitol treatment at time intervals of 0, 6, 12, and 24 hrs. PCR fragments of 566 bp and 422 bp size corresponding to <i>VrNHX1</i> and <i>VrTubβ</i> were fractionated electrophoretically on 2% agarose gel stained with 10 mg/ml ethidium bromide.</p
Effect of salt stress on germination efficiency and root growth of transgenic <i>Arabidopsis</i> lines.
No full text<p>(A) The wildtype (WT, col-0) and transgenic (line 1, 35S::<i>VrNHX1</i> and line 4, RD29A::<i>VrNHX1</i>) seedlings were observed for germination score after 10 days exposure to salt stress (150 mM NaCl). (B) Root growth inhibition in wild type (WT, Col-0) and transgenic <i>Arabidopsis</i> (Line 1, 35S::<i>VrNHX1</i> and Line 4, RD29A::<i>VrNHX1</i>) plants upon salt stress (150 mM NaCl) was studied. The 4 days old germinated seedlings were transferred to 150 mM NaCl stress for a period of 7 days and (C) root length measured was plotted in graph. Values indicate means ± SE (n = 10). Statistically significant values at P≤0.05 are indicated as “*”, using Bonferroni analysis.</p
Total intracellular ion estimation in yeast strains W303-1B, AXTYES2.0 and AXTVrNHX1.
No full text<p>Yeast cells were grown in APG medium (pH 4.0) with 1 mM KCl supplemented in presence (stressed) or absence of 75 mM NaCl (unstressed) and harvested at a cell density of 0.3. Total intracellular, vacuolar and cytoplasmic Na<sup>+</sup> and K<sup>+</sup> content was determined as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106678#s2" target="_blank">materials and methods</a> section. Data are means of 3 independent events (n = 3) and standard errors are plotted in the graph. Statistically significant values at P≤0.05 are indicated as “*”, using Bonferroni analysis.</p
Cation sensitivity assay of transformed yeast strains (W303-1B, AXTYES2.0, AXTVrNHX1) under various concentrations of NaCl, KCl, and LiCl.
No full text<p>Saturated seed cultures for each strain was diluted to an OD<sub>600</sub> of 0.006 and inoculated to liquid APGal medium (pH 5.5) supplemented with or without various concentrations of (A) NaCl (0, 50, 75, 100 mM), B) KCl (0, 0.5, 0.75, 1.0 M), and (C) LiCl (0, 15, 20, 25 mM). Growth was observed at 30°C after 3 days and absorbance recorded at 600 nm. Data are means of 3 independent events (n = 3) and standard errors are plotted in the graph. Statistically significant values at P≤0.05 are indicated as “*”, using Bonferroni analysis.</p
Heterologous expression of <i>VrNHX1</i> in yeast mutant.
No full text<p>Wild type (W303-1B) strain was used as a control, <i>Δ ena1- 4 Δnha1 Δnhx1</i> mutant (AXT3) strain was transformed with null pYES2.0 (labeled as AXTYES2.0 strain) and pYES<i>VrNHX1</i> recombinant vector (labeled as AXTVrNHX1) were used for complementation assay. 10-fold serial dilutions of saturated seed cultures of each strain were spotted onto APGal media (pH-5.5) supplemented with or without (A) 50, 75 and 100 mM NaCl, (B) 25 mM LiCl, and (B) 0.5 M KCl. (C) Hygromycin sensitivity assay was performed by spotting 10-fold serial dilutions of saturated seed cultures of each strain onto YPGal media (pH- 5.5) supplemented with or without 50 µg/ml Hyg. The plates were incubated at 30°C for 3 days.</p
