68 research outputs found
Reduction of vanadate to vanadyl by a strain of Saccharomyces cerevisiae
No full textThree strains of Saccharomyces cerevisiae, SC-1, DBVPG 6173 and DBVPG 6037, were studied for vanadate resistance in complex Sabouraud medium since they did not thrive in different minimal media (yeast nitrogen base with and without amino acids). The strain SC-1 was resistant up to 16 mM of vanadate, whereas the strains DBVPG 6173 and DBVPG 6037 were inhibited by 8 mM and 4 mM vanadate, respectively. The vanadate resistance in strain SC-1 was constitutive and due to the reduction of this oxyanion to vanadyl, which was detected by EPR spectroscopy and visible spectroscopy. The transformation of vanadate to vanadyl took place during the exponential growth phase; 10 mM of vanadate was reduced to vanadyl outside the cells since the oxyanion was not detected in the cell biomass and only a negligible concentration of vanadyl (25 nmoles mg(-1) cells dry weight) was found in the biomass. The other two vanadate-sensitive yeast strains only accumulated vanadate and did not reduce the oxyanion to vanadyl
Naphthalene and byphenyl oxidation by two Pseudomonas strains isolated from Venice lagoon sediment.
No full textA sediment sample from Venice Lagoon was found to be contaminated with 475 mgKg1 polycyclic aromatic hydrocarbons (PAHs). Naphthalene was the principal pollutant at 26% of total PAHs. Two strains of Pseudomonas SN1 and SB1 were isolated from sediment amended with 2% naphthalene. 16S rRNA gene sequence analysis indicated that the two strains have about 99% nucleotide identity with strains of the genus Pseudomonas, and are very close to Pseudomonas stutzeri. Their metabolic profiles showed significant nutritional differences, the most significant of which was that SN1 grows in marine mineral medium spiked with naphthalene and SB1 grows with biphenyl as sole carbon and energy sources. Pseudomonas sp. SN1 had a doubling time of 3.1 h with 2% naphthalene and SB1 had a doubling time of 19.5 h with 2% biphenyl. Strain SN1 oxidised naphthalene at 564732 mgO2 l1 d1 and SB1 oxidised biphenyl at 426725 mgO2 l1 d1 in respirometry reaction vessels under controlled conditions. Screening of the two strains for dioxygenase genes involved in the first step of the two hydrocarbon degradation pathways, by polymerase chain reaction, showed naphthalene dioxygenase in SN1 and biphenyl dioxygenase in SB1. The strains each have a different catechol 2,3-dioxygenase responsible for cleavage of the aromatic ring
Production of methyl mercury by sulphate-reducing bacteria in sediments from the Orbetello lagoon in presence of high macroalgal loads
No full textMethylmercury is a potent neurotoxin affecting shallow-water ecosystems. Mercury polluted sediment samples were collected at six different sites in the Orbetello Lagoon (central Italy) characterized by high levels of silt, iron, manganese hydroxides, and organic matter originated the latter originated from the decomposition of macroalgae. Porous water pointed out the presence of sulphates, methylmercury, and sulphides. Slurries arranged in anaerobic conditions from sediment aliquots from the six sites, with the addition of ionic mercury, highlighted the production of methylmercury. Sulphate reducing bacteria (SRB) were quantified in lagoon sediments; furthermore, sediments cultured under anaerobic conditions showed SRBs active in mercury methylation. Anaerobic cultures of SRB, amended with ionic mercury, produced methylmercury during the growth of bacterial cells. The percentage of aerobic mercury resistant bacteria was pointed out at each sampling site, evidencing the presence of bioavailable mercury. Several aerobic mercury resistant bacteria were isolated and their level of resistance to inorganic and organic forms of mercury was evaluated. These isolates may be potentially used for eventual bioremediation processes. Mercury methylation by SRB in the Orbetello Lagoon sediments was described for the first time, focusing the attention on the need for possible bioremediation processes by using autochthonous mercury resistant bacteria. Moreover, the influence of algal biomass on mercury methylation was highlighted for the first time in this lagoon ecosystem. The importance of removing algal biomass, as it represents a source of organic matter favouring the process of mercury methylation, was strongly pointed out in this stud
Envelope glycosilation determined in microscopy sections by lectines in Acinetobacter venetianus induced by diesel fuel.
No full textArsenic-resistant Pseudomonas spp. and Bacillus sp. bacterial strains reducing As(V) to As(III), isolated from Alps soils, Italy
No full textFive arsenic-resistant bacterial strains (designated
MP1400, MP1400a, MP1400d, APSLA3, and BPSLA3)
were isolated from soils collected at the Alps region (Italy),
which showed no contamination by arsenic. Phylogenetic
analysis of the 16S rRNA gene sequences assigned them to the
genera Pseudomonas and Bacillus. Bacillus sp. strain 1400d
and Pseudomonas spp. strains APSLA3 and MP1400 showed
higher tolerance to As(III), as indicated by minimum inhibitory
concentrations of 10 mmol/L. Pseudomonas sp. strain MP1400
exhibited higher tolerance to As(V) (minimum inhibitory
concentration of 135 mmol/L). The isolated arsenic-resistant
strains were able to reduce As(V) to As(III), especially
Pseudomonas sp. strain MP1400 reducing 2 mmol/L of As
(V) to As(III) within 24 h. The results suggest that the
isolated bacterial strains play a role in the arsenic biogeochemical
cycle of arsenic-poor soils in the Alps mount area
Una sapienza straniera. Filosofia ed ebraismo nel Medioevo
No full text"Sapienza straniera" (Hokmah Zarah) è il modo in cui la filosofia viene definita nei testi antichi della tradizione ebraica, Mishna e Talmud, ma davvero la filosofia è estranea all'ebraismo? il testo propone un'inedita presentazione della filosofia ebraica, partendo dalla convinzione che per comprendere quest'ultima sia necessario soffermarsi su quell'entroterra culturale, rappresentato essenzialmente dalla tradizione orale e dai testi fondanti in cui essa sfocia, che fa da sfondo ma anche da presupposto alla filosofia stessa. L'autrice evidenzia come alcuni elementi dell'ebraismo e dell'antica ermeneutica del testo biblico risuonino nei testi dei filosofi ebrei di svariate epoche e in tal modo riannoda dei fili, spesso sottili e nascosti, tra la filosofia e la ricca e millenaria cultura ebraica.the text proposes an unprecedented presentation of Jewish philosophy, starting from the conviction that to understand the latter it is necessary to dwell on that cultural hinterland, represented essentially by the oral tradition and the founding texts in which it flows, which serves as a background but also from prerequisite to philosophy itself. The author highlights how some elements of Judaism and of the ancient hermeneutics of the biblical text resonate in the texts of Jewish philosophers of various ages and in this way reintroduces threads, often subtle and hidden, between philosophy and the rich and millenary Jewish cultur
CALABAI SUKU BUGIS DALAM NOVEL CALABAI KARYA PEPI ALBAYQUNIE
No full textAbstract
This study focuses on the disclosure of matters relating to the existence of calabai as gender variation in Bugis ethnic which is illustrated in Calabai by Pepi Al-Bayqunie. This study is a library research with a qualitative approach so that it focuses on the text used as the object of research, namely Calabai by Pepi Al-Bayqunie. The data collection was carried out in several stages, including the observation stage, primary data recording stage, secondary data collection stage. The data analysis was carried out through several stages, namely the data classification stage, data analysis, and the conclusion of the analysis results. The results of this study indicate that there are gender variations illustrated in Calabai by Pepi Al-Bayqunie. Gender variations in the Bugis ethnic described in the novel are calabai. In the Bugis culture of South Sulawesi, the existence of calabai is a form of gender variation besides men or women. Calabai described by the author in the novel is a calabai who has special features and eventually he becomes a bissu. The main character is described as a calabai who later becomes bissu. In relation to the existence of calabai and bissu drawn by novel greetings, it turns out that there is a refusal from the group that considers these two gender variations to be incompatible with human nature. The existence of calabai is a problem because it is contrary to the social construction that already exists in the community. However, unlike calabai, the existence of bissu is considered more honorable because it plays an important role in Bugis culture.
Keywords: gender variation, calabai, bissu, Bugis ethni
Environmental quality assessment of the marine reserves of the Tuscan Archipelago, Central Tyrrhenian Sea (Italy)
No full textThere has been a worldwide increase in the number of Marine Protected Areas and marine reserves over the last decade. In these areas, the protection measures adopted are related to specific management goals; nevertheless, actual knowledge of the effectiveness of the restrictions is far from exhaustive. This article aims to contribute to knowledge of the environmental quality of the marine reserves in the Tuscan Archipelago (Mediterranean central area) which is composed of seven islands at different levels of protection. A monitoring programme spanning multiple years was performed on water and sediment samples to finalise a definition of the trophic levels and the response of microbiological indicators (total heterotrophic bacteria, Actynomyces, hydrocarbon-degrading bacteria) to persistent organic pollutants (polychlorinated biphenyls, 1,1-bis-(4-chlorophenyl)-2,2-dichloroethene, hexachlorobenzene and polybrominated diphenyl ethers) was assessed. The results showed that these reserves were generally of good quality. A clear fingerprint produced by human activities along the coast and significant differences relating to the level of protection were observed. There exists the need to also consider basin dynamics when planning the protection management strategies adopted for marine reserves
PENGAKUAN CALABAI: SEBUAH ANALISIS INTERTEKSTUAL NOVEL PASUNG JIWA KARYA OKKY MADASARI DAN NOVEL CALABAI KARYA PEPI AL-BAYQUNIE
No full textThis study aims to describe the intertextuality relationship between the novel Pasung Jiwa by Okky Madasari and Calabai by Pepi Al-Bayqunie. The type of research is descriptive qualitative approach using content analysis. Data are collected by inventorying events that are similarities and differences, specifications on the characters, settings, plots, and themes of both text. The research results indicate that there are similar themes on the two novels, the theme of self actualization in addition with the theme of family and friendship. The same characterization are also used by both author, masculine figures with feminine soul characters. The difference between the two novels lies on the plot and setting. Pasung Jiwa uses progressive plot and Calabai uses a flash-back plot.. Okky Madasari takes Java Island as the background in the novel Pasung Jiwa, while the novel Calabai, Pepi Al-Bayqunie using the setting of Sulawesi Island. The basis of the similarity of theme and characterization supported by the similirity of events in the story shows the existence of intertextual relationship between the two novels. As a previously published work, the novel Pasung Jiwa by Okky Madasari is a hipogram and novel Calabai by Pepi A-Bayqunie as a transformational text. On the theme and characterization, the transformation of Calabai forward the hypogram, while in the plot and setting deviates his hypogram, Pasung Jiwa.Penelitian ini bertujuan untuk mendeskripsikan hubungan intertekstualitas antara novel Pasung Jiwa karya Okky Madasari dan novel Calabai karya Pepi Al-Bayqunie. Jenis penelitian ini adalah deskriptif kualitatif dengan pendekatan konten analisis. Data dikumpulkan dengan menginventariskan peristiwa yang merupakan persamaan dan perbedaan, spesifikasi pada tokoh, latar, alur, dan tema dari kedua teks. Hasil penelitian ini menunjukkan bahwa terdapat kesamaan tema pada kedua novel, yaitu tema aktualisasi diri, ditambah dengan tema keluarga dan persahabatan. Penokohan yang sama juga digunakan oleh kedua penulis, yaitu tokoh maskulin dengan karakter jiwa feminin. Perbedaan kedua novel terletak pada alur dan latar. Pasung Jiwa menggunakan alur maju dan Calabai menggunakan alur campuran. Latar dalam novel Pasung Jiwa, Okky Madasari mengambil latar Pulau Jawa, sedangkan novel Calabai, Pepi Al-Bayqunie menggunakan latar Pulau Sulawesi. Dasar kesamaan tema dan penokohan didukung kesamaan peristiwa-peristiwa dalam cerita menunjukkan adanya hubungan intertekstual antara kedua novel. Sebagai karya yang terbit terlebih dahulu menjadikan novel Pasung Jiwa karya Okky Madasari adalah hipogram dan novel Calabai karya Pepi Al-Bayqunie sebagai teks transformasi. Pada tema dan penokohan, transformasi Calabai meneruskan hipogram, sedangkan pada alur dan latar menyimpangi hipogramnya, Pasung Jiwa.</p
A Shrine of Pepi I in South Abydos
No full text As part of the 2010 season of the Ahmose and Tetisheri Project in South Abydos, the author led a brief archaeological survey in the low desert south as far as Sinki. Among other archaeological sites found during this survey, the most interesting discoveries included two rock-cut chambers on the limestone escarpment. Two repeating inscriptions were found in the northernmost chamber. An ỉr.n=f m mnw=f formula and a label identify all or part of this site as a ‘shrine’ ( zḥ) built by Pepi I for the local god Khentiamentiu. The inscriptions use Pepi’s ‘Son of Hathor of Dendera’ name, which may help to date the shrine’s construction to the early part of his reign. The article describes this archaeological site, discusses the inscriptions and offers some interpretation for their historical and archaeological context as part of the activities of the Sixth Dynasty in Abydos. </jats:p
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