196,089 research outputs found
Impact of Dermoscopy and Reflectance Confocal Microscopy on the Histopathologic Diagnosis of Lentigo Maligna/Lentigo Maligna Melanoma
No full textBACKGROUND: Equivocal pigmented lesions of the head are usually biopsied to avoid inappropriate treatment. Clinical approach has evolved from simple visual examination to sophisticated techniques for selecting the biopsy sites. OBJECTIVE: This study aimed to retrospectively evaluate the efficiency of dermoscopy (DE) and reflectance confocal microscopy (RCM) in sampling a histopathologically representative focus of lentigo maligna/lentigo maligna melanoma. METHODS: Punch biopsies and surgical excisions of 72 patients, 37 men and 35 women (median age 70.6 years, range 39-90 years), affected by lentigo maligna/lentigo maligna melanoma of the head, sent from a single dermatology clinic, were reviewed for the presence of 5 histopathologic criteria: atypical junctional melanocytes, increased junctional melanocytes, follicular colonization, pagetoid spread and melanocytic junctional nests, plus other minor features. Forty-two patients were biopsied under DE and 30 under RCM guidance. RESULTS: Accuracy of the 2 techniques in sampling a representative tissue overlapped in most cases, although RCM selected sites to biopsy with more histopathologic criteria, in particular pagetoid spread and melanocytic nests. Interestingly, with RCM, inflammation and melanophages were observed more in biopsy than in excision. False positive cases were not registered. CONCLUSION: Compared with the sampling at naked eye, our results show that DE and RCM help selecting the most appropriate areas for biopsies, thus allowing not only more robust histopathologic diagnoses, but also a more accurate microstaging of tumor
Bulletin of breast disease: results of the 1990-1994 quinquennium
No full textAll mammary lesions diagnosed at the Institute of Pathological Anatomy of the University of Modena have been systematically filed since 1990 and reported in a bulletin, which is issued twice a year and delivered to health operators. So far, 5.188 cases of breast lesions, comprising 1.999 non-neoplastic pathologies, 1.040 benign tumors, 1.943 primary malignant neoplasms and 206 recurrences, have been filed. During the quinquennium 1990-1994, a progressive numerical reduction in diagnoses of non-neoplastic lesions coupled to an increase of benign tumors has been observed, whereas the number of primary malignant tumors has not changed. In particular, a statistically significant increase in diagnoses of carcinoma-in-situ and of fibrocystic disease associated with moderate-risk lesions (atypical hyperplasias) has been detected, whereas the number of cases of single fibrocystic disease has decreased. This reduction, however, is not significant. A slight increase of breast carcinomas smaller than 1 cm and 2 cm, coupled to a decrease of those exhibiting dimensions between 2 and 5 cm, has been found. The collection and systematic analysis of cases of mammary lesions appears to represent a useful tool to study the incidence of different breast pathologies in the general populations. It can also be viewed as a simple way to test the reliability of diagnostic methods used for selection of surgical cases
Fruit and vegetables in hypertensive women with asymptomatic peripheral arterial disease
No full textBackground and aims: Fruit and vegetables are considered a very healthy diet useful in the prevention of cardiovascular disease. The present study aims to evaluate intake of fruit and vegetables in hypertensive women and its correlation with asymptomatic atherosclerosis. Methods and results: A group of 237 women with hypertension was evaluated. Fruit and vegetables consumption were assessed by a self-administered food frequency validated questionnaire completed by an interviewer administered 24 h diet recall. They all underwent ABI. ABI measurement observed that fruit consumption was inversely associated with pre-clinical atherosclerosis suggesting a protective effect, moreover this association was stronger for vegetables. Increasing intake of vegetables was associated with a lower risk of asymptomatic PAD. Conclusions: Women with a high intake of fruit and vegetables showed less instrumental sign of preclinical peripheral atherosclerosis. Can be suggests that fruit and vegetables play an important role in prevention of atherosclerosis in pre-menopausal women
PROLIFERATING CELL NUCLEAR ANTIGEN CYCLIN IN INCIDENTAL CARCINOMA OF THE PROSTATE
No full textMonoclonal antibody to proliferating cell nuclear antigen (PCNA) has been used to identify the growth fraction in ten cases of benign prostatic hyperplasia (BPH), in 20 prostatic microcarcinomas (PMC) and in 30 cases of infiltrating prostatic carcinoma (PC). Ten year follow-up was available on all cases by means of clinical, serological, radiological and echographic examinations. The percentage of PCNA-staining nuclei was independently counted by two observers. Statistical analysis showed significant differences between PCNA/cyclin score of BPH and PMC without recurrences with respect to those of PMC with progression and of PC. PCNA immunostaining may represent a reliable method for assessing cellular proliferative activity. It may be used as a more powerful diagnostic hallmark of PMC than patterns of non-malignant microglandular proliferation and is also a useful additional test for assigning histological grades to PMC and PC. Statistical analysis indicated that PCNA/cyclin index was an independent significant prognostic indicator of predicting malignant progression (P less-than-or-equal-to 0.01) and survival rates (P less-than-or-equal-to 0.05) of PC and PMC (> 5 mm diameter)
Physical activity in premenopausal women with asymptomatic peripheral arterial disease
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Psoriasis vs allergic contact dermatitis in palms and soles: a quantitative histologic and immunohistochemical study.
No full textA systematic histologic and immunohistochemical study of cases of psoriasis (PSO) and allergic contact dermatitis (ACD) in palmo-plantar skin was performed to find differences between these two diseases that usually show overlapping features in these specific sites. Skin biopsies from 42 (22 female, 20 male) patients were evaluated for several histopathologic parameters and immunohistochemistry was applied to quantify keratinocytic proliferation, the number of dendritic cells (DCs) and the phenotype of the mononuclear cell infiltrate. Regular epidermal hyperplasia and marked parakeratosis were found to be more frequent in PSO than in ACD cases, but only the first parameter reached the level of significance (p = 0.03). The number of S100 protein-positive DCs was significantly higher in ACD (p = 0.006), whereas keratinocytic proliferation, studied with Mib-1, was found to be higher in PSO than in ACD, but the difference was not statistically significant. No significant difference was detected in the number of CD4+, CD8+ and bcl2+ lymphocytes in PSO and ACD cases. In the palms and soles, the finding of irregular epidermal hyperplasia and the detection of a higher number of S100 protein-positive DCs favor the diagnosis of ACD over PSO. The differential diagnosis between PSO and ACD can be practically approached using a histopathologic parameter and a commercially available antibody
Atypical fibroxanthoma and pleomorphic dermal sarcoma: A reappraisal
No full textAtypical fibroxanthoma (AFX) and pleomorphic dermal sarcoma (PDS) share clinical, pathological, immunohistochemical and molecular features, though PDS is associated with a more aggressive behavior
Relationship between Mediterranean diet and asymptomatic peripheral arterial disease in a population of pre-menopausal women.
No full textAbstract Background and aims: The Mediterranean Diet (MedD) is considered a very healthy
diet useful in the prevention of cardiovascular disease. The present study aims to evaluate adherence
to MedD in unselected premenopausal women and its relation with ankle-brachial index
(ABI), an index of preclinical atherosclerosis.
Methods and results: A group of 425 patients (age range 45e54 years) was investigated. They
were enrolled only if they were asymptomatic for cardiovascular disease. Nutritional parameters
were assessed by a self-administered food frequency validated questionnaire (116 items)
completed by an interviewer administered 24 h diet recall. They all underwent ABI measurement.
The mean MedD Score was 32.2 6.1 (Q1eQ3 range 26e37) comparing with data from
Italian population (46 8.3) was significantly lower. Intake of food categories sources of antioxidants
was higher in patients with a greater adherence to Med D and was mainly related to fruit
and vegetables. Patients were categorized in quartile according to MedD Score and we evaluate
the distribution of ABI index within quartile. 31.4% of women in Q1 (lower adherence to MedD)
had an ABI lower than 0.9 compared to 18.3% of women in Q4 (higher adherence to MedD):
p < 0.01. Obesity was more frequent in Q1 compared to Q4 and in women with lower ABI.
Conclusions: Women with a low MedD Score were more obese and showed instrumental sign of
preclinical peripheral atherosclerosis. MedD rich in antioxidants from fruit, vegetables and nuts
influenced the development of atherosclerosis and was associated with a lower incidence of
asymptomatic atherosclerosis
Relationship between quantitative expression of protein p120 and proliferative activity in cancer cells
No full textIn questo articolo, il nostro gruppo tra i primi a livello europeo indirizza la ricerca verso la p120, proteina nucleolare con notevoli potenzialità sulla cinetica di crescita tumorale
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