79,506 research outputs found

    Disruption of the developmental programme of Trypanosoma brucei by genetic ablation of TbZFP1, a differentiation-enriched CCCH protein

    No full text
    The regulation of differentiation is particularly important in microbial eukaryotes that inhabit multiple environments. The parasite Trypanosoma brucei is an extreme example of this, requiring exquisite gene regulation during transmission from mammals to the tsetse fly vector. Unusually, trypanosomes rely almost exclusively on post-transcriptional mechanisms for regulated gene expression. Hence, RNA binding proteins are potentially of great significance in controlling stage-regulated processes. We have previously identified TbZFP1 as a trypanosome molecule transiently enriched during differentiation to tsetse midgut procyclic forms. This small protein (101 amino acids) contains the unusual CCCH zinc finger, an RNA binding motif. Here, we show that genetic ablation of TbZFP1 compromises repositioning of the mitochondrial genome, a specific event in the strictly regulated differentiation programme. Despite this, other events that occur both before and after this remain intact. Significantly, this phenotype correlates with the TbZFP1 expression profile during differentiation. This is the first genetic disruption of a developmental regulator in T. brucei. It demonstrates that programmed events in parasite development can be uncoupled at the molecular level. It also further supports the importance of CCCH proteins in key aspects of trypanosome cell function

    Pelochrista wrighti Gilligan & Matthews & Miller 2018, sp.n.

    No full text
    <i>Pelochrista wrighti</i> Gilligan and Matthews, sp.n. (Figs. 4–6, 8) <p> <b>Diagnosis</b>. The distinctive male genitalia place <i>P. wrighti</i> in <i>Pelochrista</i> in the <i>canana</i> group (species 78–87 in Wright and Gilligan 2015). <i>Pelochrista wrighti</i> can be separated from all other members of the group by the lack of a welldefined ocellus and the white and brown fasciate pattern on the forewing.</p> <p> <b>Description</b>. <b>Male</b> (Figs. 4-6, 8). <i>Head</i>: Frons white; vertex white with mix of brown scales laterally; labial palpus length ca. 1.25 × horizontal diameter of compound eye, first two segments light pale brown dorsolaterally, white ventrally, third segment solid brown; antenna light pale brown; scape white. <i>Thorax</i>: Dorsal surface and tegula white with mottled patches of brown and light brown; pro- and mesothoracic legs brown, metathoracic leg pale brown to white, tarsi with white annulations. Forewing (Figs. 4–6) length 6.5–7.6 mm (mean 7.1 mm; n = 3), AR = 2.74; costal fold present along basal 1/3 of costa; ground color brown; wing markings white and brown; costal strigulae strongly expressed distad of costal fold (pairs 3–9); subbasal fascia dark brown, continuous from dorsum to radius; striae associated with costal strigulae pairs 5–6 dislocated distally and confluent with stria 7, separating the median and postmedian fasciae into narrow bands and a conspicuous dark brown pretornal patch; postmedian band and preterminal fascia indistinct patches or bands; ocellus absent. Hindwing brown, fringe scales brown basally, pale grayish brown apically. <i>Abdomen</i>: Genitalia (n = 2) (Fig. 8) with uncus evenly rounded, densely setose, weakly differentiated from dorsolateral shoulders of tegumen; socii long, densely setose, narrowed distally; phallus relatively long, tapering gradually, with base loosely surrounded by anellus; vesica with 9–10 deciduous cornuti; valva with costal margin weakly concave to nearly straight, ventral emargination moderate, NR = 0.35, saccular corner acute, mean SA = 76°; valval neck with subcostal line of hairlike setae; cucullus with dorsal lobe weakly developed and rounded, ventral lobe triangular, anal spine stout, medial surface covered in coarse setae.</p> <p> <b>Female</b>. Unknown.</p> <p> <b>Holotype</b> (Fig. 4). ♂, “ BAHAMAS: South Abaco, Schooner Bay Institute, vic. power substation, 26.167500°, - 77.18900°, 4.vi.2016, J. Miller, G. Goss, M. Simon, D. Matthews. Bahamas Survey, MGCL Accession # 2016-09. TMG 729 Genitalia dissection. MGCL 246573 McGuire Center for Lepidoptera & Biodiversity, FLMNH, UF [barcode]” MGCL.</p> <p> <b>Paratypes</b> (Figs. 5–6). BAHAMAS: N. Andros, Stafford Creek, Love at First Sight (Motel, at MVL), 24.901449°, - 77.936089°, 18 m, 28.x.2011, J. Y. Miller, M. Simon, G. Goss, D. Matthews. J. Y. Miller et al. Bahamas Survey MGCL Accession No. 2011-32. MGCL 233027 McGuire Center for Lepidoptera & Biodiversity, FLMNH, UF (1 ♂ MGCL); Grand Bahama Island, Freeport, Sea Gate Ln., 26.504418°, -78.650936°, 24.x.2014, J. Miller, M. Simon, R. Rozycki, D. Matthews. Bahamas Survey MGCL Accession No. 2014-31. D. Matthews Genitalia Prep. # 1806. MGCL 238166 McGuire Center for Lepidoptera & Biodiversity, FLMNH, UF (1 ♂ MGCL).</p> <p> <b>Etymology</b>. The species name is in honor of Donald J. Wright, whose tireless work on <i>Eucosma</i> and <i>Pelochrista</i> for the past 20 years has provided us with an understanding of these otherwise impossible groups.</p> <p> <b>Distribution and Biology</b>. This species has been recorded from three islands in the Bahamas, with adults captured in June and October. Larval hosts are unknown. Specimens were collected in or near relict or larger tracts of rocky pineland dominated by <i>Pinus caribaea</i>. The holotype was collected in a relatively pristine tract of pineland on the outskirts of a recent housing development.</p>Published as part of <i>Gilligan, Todd M., Matthews, Deborah L. & Miller, Jacqueline Y., 2018, Two new species of Eucosmini from the Bahamas (Lepidoptera: Tortricidae), pp. 265-268 in Zootaxa 4378 (2)</i> on pages 266-268, DOI: 10.11646/zootaxa.4378.2.5, <a href="http://zenodo.org/record/1169523">http://zenodo.org/record/1169523</a&gt

    Eucosma bahamae Gilligan & Matthews & Miller 2018, sp.n.

    No full text
    <i>Eucosma bahamae</i> Gilligan and Matthews, sp.n. (Figs. 1–3, 7) <p> <b>Diagnosis</b>. Although the female is unknown, <i>E. bahamae</i> is confidently placed in <i>Eucosma</i> in the <i>refusana</i> group (Wright and Brown 2014; species 16–24 in Wright and Gilligan 2015), based on similarity of male genitalia and forewing markings. It can be separated from other members of the group by its forewing pattern: uniform pale yellow with mottled grayish-brown to orange-brown markings, variably expressed lustrous gray striae, and a large salt-andpepper-colored ocelloid region surrounding the ocellus. In other members of the group with a similar ocelloid region the forewing is visibly divided into proximal (basal two-thirds) and distal sections.</p> <p> <b>Description</b>. <b>Male</b> (Figs. 1–3, 7). <i>Head</i>: Frons brown to straw yellow; vertex brown to straw yellow with many scales brown apically; labial palpus length subequal to diameter of compound eye, first two segments orange brown to dark brown dorsally, straw yellow ventrally, third segment solid orange brown to dark brown; antenna orange brown to dark brown; scape brown to dark brown. <i>Thorax</i>: Dorsal surface and tegula pale brown with scales dark-tipped to uniform straw yellow; pro- and mesothoracic legs mottled brown to orange brown, metathoracic leg pale yellow to white, tarsi with brown annulations. Forewing (Figs. 1–3) length 4.9–5.8 mm (mean 5.4 mm; n = 3), AR = 2.86; costal fold absent; ground color pale yellow; wing markings grayish brown to orange brown, scales often with dark apices; costal strigulae weakly expressed in distal one third of wing, obscured into a continuous narrow dark band along the costa in the proximal two thirds; basal, subbasal, and median fasciae weakly to moderately expressed as indistinct mottling; striae lustrous gray to silver, variably expressed in distal one third of wing, stria number 6 extending nearly continuously from costa along proximal margin of ocelloid region to dorsum; ocelloid region extending from tornus to radius, ringed with lustrous gray and fragments of striae; scales in ocelloid region and along termen white basally, dark brown medially, and white apically, producing a salt-and-pepper effect; ocellus with three rows of four black dashes on a white background separated medially by a lustrous gray vertical bar. Hindwing white to pale brown with pale grayish brown scales tracing veins; elongate fringe scales white, subtended by a layer of shorter, grayish scales. <i>Abdomen</i>: Genitalia (n = 2) (Fig. 7) with uncus with basal width ca. 2 × height, well-differentiated from dorsolateral shoulders of tegumen; socii fingerlike; phallus stout, tapering distally; vesica with 7–12 deciduous cornuti; valva with costal margin nearly straight, basal setal patch weakly raised, densely covered with stiff moderately long setae, ventral emargination shallow, NR = 0.74, saccular corner broadly rounded, mean SA = 148°; cucullus with dorsal lobe moderately developed, apex rounded, distal margin weakly convex, anal angle weakly produced, basoventral margin extending in ridgelike manner onto medial surface of neck, basal excavation shallow.</p> <p> <b>Female</b>. Unknown.</p> <p> <b>Holotype</b> (Fig. 1). ♂, “ BAHAMAS: Crooked Is [land], Pittstown Point, 22.831211°, -74.348717°, 10–11.iii.2014, at light, M. Simon & M. Simon. Bahamas Survey, MGCL Accession No. 2014-8. TMG 728 Genitalia dissection. MGCL 233071 McGuire Center for Lepidoptera & Biodiversity, FLMNH, UF [barcode]” MGCL.</p> <p> <b>Paratypes</b> (Figs. 2–3). BAHAMAS: Long Island, vic. Salt Pond, 23.353833°, -75.119500°, 30.v.2014, J. Miller, G. Goss, M. Simon, D. Matthews. Bahamas Survey MGCL Accession No. 2014-14. MGCL 234324 McGuire Center for Lepidoptera & Biodiversity, FLMNH, UF (1 ♂ MGCL); Cat Island, vic. Ocean Dream Resort, E of Smith Town, 24.352295°, -75.454510°, 23.vi.2014, J. Miller, M. Simon, D. Matthews, G. Goss. Bahamas Survey MGCL Accession No. 2014-15. D. Matthews Genitalia Prep. # 1826. MGCL 238600 McGuire Center for Lepidoptera & Biodiversity, FLMNH, UF (1 ♂ MGCL).</p> <p> <b>Etymology</b>. The species name refers to the Bahamas, the only location from which this species has been collected.</p> <p> <b>Distribution and Biology</b>. This species has been recorded only from islands in the Bahamas. Adults were captured in March–June. Larval hosts are unknown. Specimens were collected in open disturbed areas less than 0.1 mile from the shoreline with dune vegetation present.</p>Published as part of <i>Gilligan, Todd M., Matthews, Deborah L. & Miller, Jacqueline Y., 2018, Two new species of Eucosmini from the Bahamas (Lepidoptera: Tortricidae), pp. 265-268 in Zootaxa 4378 (2)</i> on page 266, DOI: 10.11646/zootaxa.4378.2.5, <a href="http://zenodo.org/record/1169523">http://zenodo.org/record/1169523</a&gt

    Leaus Matthews & Lawrence 1992

    No full text
    Leaus Matthews & Lawrence, 1992 (Figs 5, 10, 20 –36, 39, 40, 44, 47, 48, 50, 51, 54, 56) Type species. Leaus tasmanicus Matthews & Lawrence, 1992, by monotypy. Redescription. Adults. With the characteristics of the tribe. Length 3.9–8.4 mm. Body parallel-sided, 2.2 –3.0 times as long as wide. Upper surfaces nitid fuscous or castaneous, clothed with recumbent white setae forming patterns on dark background, also with long sparse erect pilosity. Head: Basal membrane of labrum narrow but exposed and complete. Eyes entire, broadly oval. Antennae short, not quite reaching base of prothorax, antennomeres obconic, gradually widening distally, apical segment large and suboval. Mentum small, subquadrate or a little transverse. Prothorax: Pronotum densely punctate, moderately convex, lateral carinae present. Procoxal cavities internally open. Trochantins absent. Pterothorax: Elytral disc at least slightly uneven, without striae, with partly effaced puncture rows. Wings fully developed. Legs: Procoxae subconical, approximated, projecting well below prosternum. Tarsi about as long as tibiae. Abdomen: Intercoxal process narrowly triangular. First three ventrites connate. Defensive glands present, small, widely separated. Female: Ovipositor with paraprocts elongate, about twice as long as coxites, baculi of first coxite lobes diagonal, short, coxites with four distinct pairs of lobes. Spermatheca coiled, attached to base of accessory gland. Single vaginal sclerite with median keel and transverse lateral wings. Description. Larva. With the characteristics of the tribe. Dorsal surfaces bicolored with a complex pattern of dark brown markings on a yellow background. Head with sides subparallel (Fig. 40); larger anterior epipharyngeal sensilla (Fig. 54) with anterior 2 moderately widely separated and well separated from the posterior 4, which form a subquadrate cluster; posterior sensilla forming two longitudinal rows of 4; mandibles bidentate with short subapical tooth on incisor edge; left mola with several weak transverse ridges; gula 3 times as long as wide; hypopharyngeal sclerome anteriorly trilobed. Prothorax almost as long as wide. Legs slightly longer than thoracic width and clothed with fine hairs. Abdomen about 3.5 times as long as thorax; tergum I with weak anterior carina; tergum IX (Fig. 47) slightly shorter at midline and slightly narrower than VIII, carina simple; urogomphi (Fig. 48) each with 4 setiferous tubercles at about middle; surface of concave disc lightly pigmented, dark-rimmed pits less regularly spaced and concentrated around a pair of posterolateral impressions; segment X with a pair of tubular pygopods (Fig. 44). Spiracles annular-cribriform with the peritreme completely surrounded by a cribriform plate (Fig. 56). Larval material examined: Leaus tasmanicus Matthews & Lawrence. AUS: Tasmania: Pelion (41.50 S, 146.08 E), 1991, pyrethrin knockdown, rainforest, P. Greenslade & M. Comfort (ANIC). Discussion. At the time of its description Leaus was placed in Heleini because it shows the basal tenebrionine characteristics of that tribe and, in particular, similarities to the heleine genus Lepispilus. Both Leaus and Lepispilus have pale dorsal setae which form a mottled pattern, and they share the unusual character of a vaginal sclerite. At the same time, it was mentioned that there is a resemblance between Leaus and Trachelostenus in the form of prominent coxae. Later, Matthews (2003) transferred Leaus to Titaenini, another basal tribe of Tenebrioninae, as discussed in the introduction. We now know that Titaenini as then conceived by Matthews was a paraphyletic assemblage of primitive Tenebrioninae sharing mainly plesiomorphies. Lepispilus differs from Leaus in the absence of prominent coxae, presence of a medial fleck on the wings, complete elytral epipleura, aedeagal alae not of the appendiculate type, and unique ovipositor structure. The ovipositor of Lepispilus (illustrated in Matthews & Bouchard 2008, fig. 47 C) does not resemble that of any other known tenebrionid. There is therefore no evidence of a direct relationship between Lepispilus and Leaus or any other trachelostenine. The peculiar type of spiracular opening surrounded by a cribriform plate in larvae of Leaus (called annularcribriform by Lawrence et al. 2011) is perhaps an adaptation to wetter habitats and has been found sporadically in various other unrelated families of Coleoptera including Trogossitidae-Lophocaterinae, Mycteridae-Hemipeplinae, and Chrysomelidae-Cryptocephalinae.Published as part of Matthews, Eric G. & Lawrence, John F., 2015, Trachelostenini sensu novo: redescriptions of Trachelostenus Solier, Myrmecodema Gebien and Leaus Matthews & Lawrence, based on adults and larvae, and descriptions of three new species of Leaus (Coleoptera: Tenebrionidae), pp. 289-312 in Zootaxa 4020 (2) on pages 301-304, DOI: 10.11646/zootaxa.4020.2.4, http://zenodo.org/record/23647

    Reconciliation as a resource for critical pedagogy

    No full text
    Reconciliation is one of the most significant contemporary challenges in the world today. In this innovative new volume, educational academics and practitioners across a range of cultural and political contexts examine the links between reconciliation and critical pedagogy, putting forward the notion that reconciliation projects should be regarded as public pedagogical interventions, with much to offer to wider theories of learning.Robert Hattam and Julie Matthew

    B. A. Hepple et M. H. Matthews, Tort. Cases and Materials, 2e éd.

    No full text
    B. A. Hepple et M. H. Matthews, Tort. Cases and Materials, 2e éd.. In: Revue internationale de droit comparé. Vol. 33 N°1, Janvier-mars 1981. p. 215

    Short Multipliers for the Extended Gcd Problem

    No full text
    For given non{zero integers s 1 ; : : : ; s m , the problem of nding integers a 1 ; : : : ; am satisfying s = gcd (s 1 ; : : : ; s m ) = a 1 s 1 + + am s m , with a 2 1 + +a 2 m minimal, is thought to be computationally hard. In this paper, we present an algorithm which takes as its starting point the recent LLL{based algorithm of Havas, Majewski and Matthews and which often nds a shorter vector (a 1 ; : : : ; am ). 1 Introduction Let s 1 ; : : : ; s m be integers and s = gcd (s 1 ; : : : ; s m ). In a recent paper [Havas, Majewski, Matthews 1998], the author and his collaborators used variants of the LLL algorithm to nd multiplier vectors (a 1 ; : : : ; am ) of small Euclidean length jjXjj = (a 2 1 + + a 2 m ) 1=2 such that s = a 1 s 1 + : : : + am s m . In each case a unimodular m m matrix P is produced such that P [s 1 ; : : : ; s m ] t = [0; : : : ; 0; s] t . Rows p 1 ; : : : ; p m 1 of P constitute a basis of short vectors for the (m 1){dime..

    B. A. Hepple et M. H. Matthews, Tort. Cases and Materials, 2e éd.

    No full text
    B. A. Hepple et M. H. Matthews, Tort. Cases and Materials, 2e éd.. In: Revue internationale de droit comparé. Vol. 33 N°1, Janvier-mars 1981. p. 215

    Leaus tropicalis Matthews & Lawrence, 2015, sp. n.

    No full text
    <i>Leaus tropicalis</i> sp. n. <p>(Figs 10, 20, 24, 28, 29, 35)</p> <p> <b>Description</b>. With the characteristics of the genus. Length 4.0– 4.5 mm, maximum width of prothorax 1.0– 1.2 mm, width at humeri 1.2–1.6 mm. <i>Prothorax</i> with sides evenly convex and lateral carinae strongly irregularly dentate, disc evenly convex. <i>Pterothorax</i>. Elytra slightly tumescent at bases. <i>Abdomen</i>: Ovipositor with terminal gonostyli. Vaginal sclerite with central keel partly divided into two blades, and lateral halberd-shaped wings separated from central keel by narrow membranes. Aedeagal parameres simple. Larva unknown.</p> <p> <b>Distribution</b>. <i>L. tropicalis</i> is found in three of the 17 generally recognised montane blocks in the Queensland Wet Tropics: the Carbine Tableland (A4), Atherton Tableland (A9) and Mount Bellenden Ker (A10) at altitudes of 1150–1500m. A4 and A10 are the areas of highest diversity and endemism, which have served as stable refugia for a long geological period (Bouchard <i>et al</i>. 2005).</p> <p> <b>Type specimens. Holotype</b> ♀: NE Q: 16o24’S x 145o13’E 3.5 km NNE Mt Spurgeon 16 Oct 1991 1330 m Monteith & Janetzki Pyrethrum, trees & rocks (QMBA T 228900). <b>Paratypes</b>: ♂, 17.28S 145.29E QLD, Longlands Gap BS1 1150m 1 Sept-2 Oct. 1995 L. Umback Malaise trap (ANIC); ♀ NEQ: 17o24’Sx 145o49’E, Bartle Frere top camp 1500m 29 Nov 1998 G. Monteith Pyrethrum trees, R.F.1978 (QMBA); ♀, 17.06S 145.36E QLD, Mt Haig GS1 1150m, 3 Jan.-4 Feb.1995, P. Zborowski, Malaise traps (ANIC).</p>Published as part of <i>Matthews, Eric G. & Lawrence, John F., 2015, Trachelostenini sensu novo: redescriptions of Trachelostenus Solier, Myrmecodema Gebien and Leaus Matthews & Lawrence, based on adults and larvae, and descriptions of three new species of Leaus (Coleoptera: Tenebrionidae), pp. 289-312 in Zootaxa 4020 (2)</i> on page 310, DOI: 10.11646/zootaxa.4020.2.4, <a href="http://zenodo.org/record/236475">http://zenodo.org/record/236475</a&gt
    corecore