83 research outputs found
Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region
No full textMonteiro, Elideth Pacheco, Da Silva, Diehgo Tuloza, Sanches, Osimar, Hamoy, Igor, Matos, Edilson Rodrigues (2019): Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region. Acta Protozoologica 58 (1): 7-16, DOI: 10.4467/16890027AP.19.002.10833, URL: http://dx.doi.org/10.4467/16890027ap.19.002.1083
Figure 3 in Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region
No full textFigure 3. Schematic drawing of a spore of Kudoa viseuensis n. sp. in apical view (right) and side view (left). Scale bar = 5 μmPublished as part of Monteiro, Elideth Pacheco, Da Silva, Diehgo Tuloza, Sanches, Osimar, Hamoy, Igor & Matos, Edilson Rodrigues, 2019, Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region, pp. 7-16 in Acta Protozoologica 58 (1) on page 12, DOI: 10.4467/16890027AP.19.002.10833, http://zenodo.org/record/835694
Figure 4 in Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region
No full textFigure 4. Phylogenetic tree derived from Bayesian Inference (BI), based on the partial sequences of the SSU rDNA gene of Kudoa vi- seuensis n. sp. and closely-related myxosporans. The GenBank access numbers are shown next to the species names, and the numbers at each node are the BI posterior probabilities. The new species is highlighted in bold type. Abbreviations: Msl – muscle; Dtr – digestive tract; Nsy – nervous system.Published as part of Monteiro, Elideth Pacheco, Da Silva, Diehgo Tuloza, Sanches, Osimar, Hamoy, Igor & Matos, Edilson Rodrigues, 2019, Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region, pp. 7-16 in Acta Protozoologica 58 (1) on page 12, DOI: 10.4467/16890027AP.19.002.10833, http://zenodo.org/record/835694
Figure 1 in Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region
No full textFigure 1. Light photomicrograph: (A) Whitish pseudocyst (arrowhead) found in the musculature of B. surinamensis. Scale bar: 1000 µm; (B) pseudocyst (c) and numerous mature spores (e) observed following the rupture of the pseudocyst. Scale bar: 100 µm; (C) Fresh, pseu- do-square spores (e) of Kudoa viseuensis n. sp. Scale bar: 20 µm; Inset: polar capsules (PC) in lateral (L) and apical (A) views (DIC). Scale bar: 10 µm.Published as part of Monteiro, Elideth Pacheco, Da Silva, Diehgo Tuloza, Sanches, Osimar, Hamoy, Igor & Matos, Edilson Rodrigues, 2019, Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region, pp. 7-16 in Acta Protozoologica 58 (1) on page 10, DOI: 10.4467/16890027AP.19.002.10833, http://zenodo.org/record/835694
Figure 2 in Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region
No full textFigure 2. Light photomicrograph: (A) longitudinal histology section of the skeletal musculature of B. surinamensis containing a pseudocyst (*), along the axis of the muscle, showing the substitution of the fiber by the parasite; (B) transversal section showing the pseudocyst of the mixosporean occupying the central portion of the muscle fiber (arrowhead), typical of an individual infection; (C) Multiple infection of pseudocysts within a single muscle fiber, separated from one another and the muscle tissue by a fine conjunctive membrane (arrows). Scale bars: 40 µm.Published as part of Monteiro, Elideth Pacheco, Da Silva, Diehgo Tuloza, Sanches, Osimar, Hamoy, Igor & Matos, Edilson Rodrigues, 2019, Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region, pp. 7-16 in Acta Protozoologica 58 (1) on page 10, DOI: 10.4467/16890027AP.19.002.10833, http://zenodo.org/record/835694
Kudoa viseuensis Monteiro & Da Silva & Sanches & Hamoy & Matos 2019, n. sp.
No full text<p> <i>Kudoa viseuensis</i> n. sp.</p> <p> <b>ZooBank:</b> lsid:zoobank.org:act: AF886D76-EC95- 4EA7-8F0D-DA2D4B956A60</p> <p> Host: <i>Batrachoides surinamensis</i> (Bloch and Schnei- der, 1801).</p> <p>Infection site: Pseudocysts in the somatic musculature.</p> <p>Type locality: Brazil, state of Pará, municipality of Viseu (1°08’ S, 46°05’ W).</p> <p>Prevalence: 86% (52/60) of the examined hosts were infected.</p> <p> Etymology: the specific name, <i>viseuensis</i>, refers to the municipality of Viseu, where the specimens were captured.</p> <p>Type specimen: A glass slide with a 5 μm-thick his- tological section stained in Haematoxylin and Eosin, containing the spores of the new species was deposited in the Zoology Museum of the National Institute of Amazonian Research (INPA) in Manaus, Amazonas, Brazil, under catalog number CNIDARIA – INPA 038.</p> <p> Description of the spores: The spores of <i>Kudoa viseuensis</i> n. sp. were 7.2±0.2 μm in length and 5.2±0.2 μm in width (Figure 3). In the apical view, the polar capsules were 1.8±0.2 μm in length and 1.3±0.1 μm in width. When observed laterally, the mean length was 2.7±0.2 μm and the width was 1.3±0.1 μm (Table 1). Table 1 compares the dimensions of the spores and polar capsules (and the shape of the spores) of other <i>Kudoa</i> species with <i>Kudoa viseuensis</i> n. sp. (Cnidaria: Myxozoa: Myxosporea: Multivalvulida: Kudoidae). <i>Kudoa viseuensis</i> n. sp. was restricted to the skeletal musculature of the host, and was not found in any other organ of the host fish.</p> <p> The morphological comparisons indicated that <i>Kudoa viseuensis</i> n. sp. is most similar to <i>K. orbicularis</i> (Azevedo <i>et al</i>. 2016) in terms of the width of the spore and the polar capsule, although other dimensions are clearly distinct from those of this species, and other <i>Kudos</i> species, studies previously. These findings, to- gether with the molecular data (see below) lend support to the present description of the new species.</p> Phylogenetic analyses <p> A partial sequence of 1400 base pairs (bps) of the SSU rDNA gene was obtained from the spores of <i>Kudoa viseuensis</i> n. sp., found in the musculature of <i>B. surinamensis</i>. This sequence was deposited in GenBank under accession number MK256272. The phylogenetic tree generated by Bayesian Inference defined a major clade, denominated clade A, composed of species of the genus <i>Kudoa</i> (Figure 4), which is subdivided into two clades, denominated A1 and A2, with high support (posterior probabilities). Each of these clades was influenced strongly by the tissue tropism of the parasites, with <i>Kudoa viseuensis</i> n. sp. being included in subclade A2. Subclade A1 is composed of <i>Kudoa</i> species that parasitise the musculature, brain, and intestine of fish- es. <i>Kudoa viseuensis</i> n. sp. is included in subclade A2, which is basal to A1. <i>Kudoa viseuensis</i> n. sp. parasitizes the musculature of the marine fish <i>B. surinamensis</i>, and clusters with <i>Kudoa orbicularis</i> (Azevedo <i>et al</i>. 2016), a parasite of the musculature of the freshwater fish, <i>Chaetobranchopsis orbicularis</i>. In this arrangement, <i>K. orbicularis</i> is the sister species of <i>Kudoa viseuensis</i> n. sp., and the two species not only share the infection site, but are also found in the same geographic region.</p> <p>ND – Not available; L* – Lateral; A* – Apic</p> <p> The outgroup is composed of species of the genus <i>Unicapsula</i> Davis, 1924, a member of the order Multivalvulida Schulman, 1959, which infects the musculature of its hosts.</p> <p> A new alignment was run for the pairwise comparison of key <i>Kudoa</i> species with <i>Kudoa viseuensis</i> n. sp. (Table 2). The smallest pairwise genetic distance (p) found in this analysis between <i>Kudoa viseuensis</i> n. sp. and the other <i>Kudoa</i> species was 3.9% in the case of <i>K. orbicularis</i> (KM192365), while the greatest distance was 4.9% for <i>K. rosenbuschi</i> (AY623795).</p>Published as part of <i>Monteiro, Elideth Pacheco, Da Silva, Diehgo Tuloza, Sanches, Osimar, Hamoy, Igor & Matos, Edilson Rodrigues, 2019, Morphological and Molecular Characteristics of Kudoa viseuensis n. sp. (Myxosporea: Multivalvulida), Found in the Muscle of Batrachoides surinamensis (Teleostei: Batrachoididae) in the Brazilian Amazon Region, pp. 7-16 in Acta Protozoologica 58 (1)</i> on pages 9-13, DOI: 10.4467/16890027AP.19.002.10833, <a href="http://zenodo.org/record/8356940">http://zenodo.org/record/8356940</a>
Estudo morfológico de microparasitas em Aequidens plagiozonatus Kullander 1984 (Osteiththyes: Cichlidae)
No full textThe ornamental fish market has been growing gradually in recent years and the increasing pressure on the banks of natural fishery resources, seeks to find alternatives to the continued production of fish through aquaculture or is the search for new features or even slightly, such as fish Aequidens plagiozonatus, common name cará pixuna. Based on this knowledge is important to perform studies characterizing the parasitological profile of these species of fish in order to verify and control the spread of parasites that cause imbalance in aquatic ecosystems. The objective this study was to provide a better understanding the bioecology, through a survey of actions caused by parasitic microparasites (microsporídios, myxosporídios e coccidios) emphasizing the morphology of these organisms. For this, five collections were made, totaling 100 specimens, which were autopsied and having their organs. Parasitized organs were removed fragments, fixed and processed for light microscopy. Hepatosomatic index were calculated and the prevalence of the parasite specimens for each group of parasites analyzed, giving the enfaze microparasites. From the observations made were 3 phyla were found: Myxozoa, Microsporidia and Apicomplexa. We observed the first occurrence of mixosporidiose urinary bladder of this species. We also observed the occurrence of multiparasitism and the multifocal localization of some parasites. The prevalence of microsporidia in the studied was 100%, 18% myxosporeans, Apicomplexa 45%. These data represent an important contribution to the study sanitary fish A. plagiozonatus in the region, since any activity that involves the extraction of natural resources should be managed through planning and management measures to allow the equilibrium of ecosystems, promoting sustainable use of its resources and guaranteeing the preservation of the system, so that it can prevent the spread and transfer of disease by means of aquatic animals. Keywords: Fish, Ictioparasitology, Aequidens plagiozonatus, microparasites.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO mercado de peixes ornamentais vem crescendo gradativamente nos últimos anos e com a crescente pressão exercida nos bancos naturais de recursos pesqueiros, busca-se alternativas para a continuidade da produção de pescado, seja através da aqüicultura ou da busca por novos recursos pouco ou até mesmo inexplorado, como e o caso do cara-pix8una, Aequidens plagiozonatus. A partir desse conhecimento é importante a realização de estudos que caracterizem o perfil parasitológico dessas espécies de peixes a fim de verificar e controlar a disseminação de parasitas que geram desequilíbrio nos ecossistemas aquáticos. Por isso, o objetivo deste estudo foi Descrever morfologicamente os microparasitas (microsporídios, mixosporídios e coccidios) encontrados em A. plagiozonatus provenientes do rio Peixe-Boi /PA, através do levantamento de ações parasitárias causadas por microparasitas (microsporídios, myxosporídios e coccidios) com ênfase na morfologia destes. Para isso, foram realizadas 5 coletas, totalizando 100 espécimes, sendo estes necropsiados e tendo seus órgãos analisados. Dos órgãos parasitados foram retirados fragmentos, fixados e processados para microscopia de luz. Foram calculados o Indice hepatossomático dos exemplares e a prevalência parasitaria para cada grupo de parasitas analisados, enfatizando os microparasitas. A partir das observações feitas foram encontrados os 3 filos parasitas: Myxozoa, Microsporidia e Apicomplexa. A prevalência dos microsporídios nos exemplares estudados foi de 100%, dos mixosporidios 18% e dos Apicomplexa de 45%. Nossos dados representam uma importante contribuição para o estudo ictiossanitário em A. plagiozonatus na região, uma vez que qualquer atividade que envolva a extração de recursos naturais deve ser gerenciada por meio de medidas de ordenamento e manejo, para permitir o equilíbrio dos ecossistemas, promovendo o uso sustentável de seus recursos e garantindo a preservação do sistema, a fim de que se possa evitar a propagação e transferência de doenças por meio de animais aquáticos
Esquistossomose mansônica em Belém, Pará, Brasil
No full textA esquistossomose é uma doença infecciosa parasitária causada pelo Schistosoma mansoni em 52 países, incluindo o Brasil, onde constitui um grave problema de saúde pública. Na região Norte, desde a década de 50, há relatos de transmissão ativa de esquistossomose em Belém, sendo apontadas as espécies Biomphalaria straminea e Biomphalaria glabrata. Este estudo verificou a situação atual desta parasitose em cinco bairros de Belém (Guamá, Invasão Tocantins, Telégrafo, Montese e Sacramenta), onde foram feitas coletas de moluscos em três estações de amostragem por bairro. Os exemplares foram mensurados, examinados quanto à presença de formas larvais de trematódeos e identificados morfologicamente. Complementarmente foram analisados os casos de esquistossomose entre 2001 e 2011. O bairro que apresentou o maior número de exemplares foi Invasão Tocantins, onde foram capturados 140 exemplares durante 30 minutos. A espécie predominante foi B. glabrata encontrada em 12 estações de amostragem nos bairros Guamá, Sacramenta, Telégrafo e Montese, e B. straminea foi obtido em Invasão Tocantins e Guamá. Foram coletados 367 exemplares, sendo 100 conchas e 267 vivos, apresentando tamanhos entre 1 e 8mm. Três exemplares se apresentaram positivos para Schistosoma mansoni, obtidos em Guamá, Telégrafo e Sacramenta. Os dados coproscópicos cedidos pela Secretaria Municipal de Saúde de Belém do Programa de Vigilância e Controle da Esquistossomose demonstram transmissão ativa em Guamá, Montese, Sacramenta e Telégrafo. Este estudo confirma a necessidade de ações de vigilância epidemiológica e educação em saúde em Belém para cumprimento do que preconiza o Ministério da Saúde na eliminação desta enfermidade no território brasileiro
Aspectos morfológicos e ultraestruturais de Mixosporidiose e Coccidiose em cichla temensis Humboldt, 1821, (Osteichthyes: Cichlidae) oriundo do município de Cametá/PA.
No full textO presente estudo relata os aspectos em microscopia de luz e eletrônica de transmissão de um mixosporidio e um coccídio encontrado parasitando brânquia e fígado, respectivamente, do peixe teleósteo Cichla temensis (Humboldt, 1821), coletado no rio Tocantins, próximo à cidade de Cametá, Estado do Pará, Brasil. A prevalência de infecção foi de 60%, tanto para mixosporidiose quanto para a coccidiose. Na mixosporidiose, os cistos contendo esporos estavam localizados nas lamelas brânquias, com formato oval e coloração esbranquiçada. Os esporos tinham um comprimento total de 42,3±0,65pm; corpo fusiforme medindo 12,8+0,42 pm de comprimento e 8,6+0.32 pm de largura; cada uma das duas válvulas apresentaram uma cauda afilada medindo 29,5+0.73 pm. Os esporos apresentavam duas cápsulas polares com 7,4+0.16 pm de comprimento e 2,6+0.08 pm de largura, contendo um filamento polar com 5-7 voltas. Está sendo apresentada uma tabela com as mensurações de esporos de Henneguya do Brasil, comparados neste trabalho. Os esporos são diferentes das espécies descritas anteriormente. Com base na morfologia dos esporos, conclui-se que esta espécie
pertence à família Myxobolidae, gênero Henneguya, e que constitui uma nova espécie: H. temensis n. sp. Na coccidiose, os oocistos estavam disseminados em todo parênquima hepático, e de acordo com a morfologia observada em ML (oocisto contendo 4 espocistos, cada um com 2 esporozoítos), conclui-se que esta espécie
pertence à família Calyptosporidae, gênero Calyptospora. Esses dados permitem descrever um novo hospedeiro para Henneguya e Calyptospora, além de descrever uma nova espécie de mixosporidio em Cichla temensis.The present study reports on aspects of light microscopy and transmission electron of a mixosporidio and a coccidian parasite found in gill and liver, respectively, teleost fish Cichla temensis (Humboldt, 1821), collected the Tocantins River, near to Cameta, State Pará, Brazil. The prevalence of infection was 60% for both mixosporidiose, as for coccidiosis. In mixosporidiose cysts containing spores were located in gill lamellae with
oval and whitish. The spores had a total length of 42.3 ± 0.65 pm ; fusiform body measuring 12.8 ± 0.42 pm in length and 8.6 ± 0.32 pm in width, each of the two valves presented a tapering tail measuring 29, 5 ±0.73 pm. The spores had two polar capsules with 7.4 ±0.16 pm in length and 2.6 ± 0.08 pm in width, containing a polar filament with 5-7 turns. This is a table with the measurements of spores of Henneguya in Brazil, being compared in this work. The spores are different from previously described species. Based on spore morphology, it is concluded that this species belongs to the family Myxobolidae, genus Henneguya, and constitutes a new species: H. temensis sp. In coccidiosis, oocysts were scattered throughout the liver parenchyma, and according to the morphology observed under light microscopy (oocyst containing 4 espocistos, each with 2 sporozoites), it is concluded that this species belongs to the family Calyptosporidae, gender Calyptospora. These data allow us to describe a new host for Henneguya and Calyptospora, and describe a new species of mixosporidio in Cichla temensis
Characterization of morphologic and ultrastructural aspects of the life cycle of the microsporidia found in fish of the Amazon region
No full textThis work describes the results in the light (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM) obtained of the life cycle of some microsporidian species (phylum Microsporidia Balbiani, 1882) parasites of the ichthyofauna of the Amazon region. Emphasis special to the ultrastructural aspects of the different life cycle phases with evidence of the spores was given. The spores and life cycle stages characterized the different genus and species. The ultrastructural organization of the host tissues with the lysed aspects, that frequently occurred, and the ultrastructural aspects of the xenoma, was discussed.Este trabalho apresenta resultados obtidos em microscopia de luz (ML), microscopia eletrônica de transmissão (TEM) e microscopia eletrônica de varredura (SEM) do ciclo de vida de algumas espécies de microsporídios (phylum Microsporidia Balbiani, 1882), parasitas da fauna ictiológica da região amazônica. Especial destaque é dado aos aspectos ultra-estruturais das diferentes fases do ciclo de vida, com atenção especial para as células esporais, que são as que caracterizam os diferentes gêneros e as diferentes espécies. O tecido hospedeiro é relacionado aos aspectos de lise, que ocorrem freqüentemente, bem como aspectos ultra-estruturais de xenomas que ocorrem em certas espécies destes parasitas
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