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Brachetto
No full textAccessione Brachetto:Foto: Claudio D'Onofrio, Puntoni Piero
Ampelografia: Claudio D'Onofrio, Belluomini Paolo, Calabrò Rolando, Rocco Fausta
Ampelometria: Claudio D'Onofrio
Fenologia: Claudio D'Onofrio, Ducci Eleonora, Matarese Fabiola, Cuzzola Angela
SSR: Claudio D'Onofrio, Matarese Fabiol
Barghigiana
No full textPer la prima volta è stata individuata in Lucchesia (molto probabilmente in Garfagnana) da Basso dell'Università di Pisa (1993) che l'ha introdotta nella collezione 'Vitiarium' dell'Agricola San Felice (Siena). Recentemente è stata individuata e caratterizzata in Garfagnana, dove al momento ha una presenza sporadica, confrontata con quella presente nella collezione 'Vitiarium' confermanodone l'identità (D'Onofrio et al., 2015)
Al momento non corrisponde a nessuno dei genotipi presenti nei vari database viticoli, e inoltre presenta delle relazioni di parentela di primo grado con 'Canina bianca', 'Rossara tardiva' e 'Sillicana bianca', tutti vitigni strettamente autoctoni della Garfagnana. Pertanto, queste indicazioni, congiuntamente alle uniche citazioni storiche che la riportano in Lucchesia, si può supporre che sia effettivamente un vitigno autoctono della Garfagnana (D'Onofrio et al., 2015). Presenta una produzione tendenzialmente elevata, con grappoli medio-grandi e compatti. Fatica ad accumulare un adeguata grado zuccherino, e al contempo nel corso della maturazione l'acidità di riduce velocemente. Al momento non abbiamo dati sufficienti sulle caratteristiche tecnologiche
Functional characterization of terpene synthases of 'aromatic' and 'non-aromatic' grapevine cultivars
No full textTwo Endopolygalacturonase Genes inTrichoderma virens:In SilicoCharacterization and Expression during Interaction with Plants
No full textThe ability of Trichoderma spp. to antagonize most phytopathogenic fungi
and to interact with plants by inducing their resistance and promoting
their growth has been widely described. Endopolygalacturonases
(endoPGs) produced by Trichoderma spp. can assist root penetration and
play a pre-eliciting role in induced systemic resistance (ISR), a beneficial
effect detected in plants colonized by Trichoderma. In this study, two
endoPG genes (Tvpg1 and Tvpg2) have been identified and partially characterized
in a T. virens isolate, previously investigated for its antagonistic
ability in several biological systems. An extensive in silico analysis showed
relatively low sequence similarities (53%) between TvPG1 and TvPG2
proteins and underscored interesting phylogenetic relationships in the
comparison of endoPG genes among fungal genomes. The phylogeny of
endoPGs suggests a differentiation in action mechanisms associated with
biological functions as one cluster shows only genes from plant-interacting
organisms. A gene expression analysis demonstrated a different regulation
pattern for those genes. The Tvpg1 gene was induced both when the
fungus was grown in liquid cultures supplemented with pectin or plant
cell walls and when it was applied to tomato roots in a growth chamber.
Expression times were comparable in both systems. On the contrary, the
Tvpg2 gene displayed a constitutive expression in all conditions tested,
including controls. The molecular communication between tomato roots
and T. virens was investigated by checking the expression of a tomato PGIP
(Lepgip1) in roots colonized by fungal mycelium. The Lepgip1 transcript
was induced at times coincident with Tvpg1 expression, suggesting a correlation
between the two gene products. This paper reports the results of
first investigations of T. virens endoPG genes performed on a structural,
phylogenetic and functional level
Expression of terpene synthase genes associated with the formation of volatiles in different organs of Vitis vinifera
No full textPlants produce a plethora of volatile organic compounds (VOCs) which are important in determining the
quality and nutraceutical properties of horticultural food products, including the taste and aroma of wine.
Given that some of the most prevalent grape aroma constituents are terpenoids, we investigated the
possible variations in the relative expression of terpene synthase (TPS) genes that depend on the organ.
We thus analysed mature leaves, young leaves, stems, young stems, roots, rachis, tendrils, peduncles, bud
flowers, flowers and berries of cv Moscato bianco in terms of their VOC content and the expression of 23
TPS genes.
In terms of the volatile characterization of the organs by SPME/GC–MS analysis, flower buds and open
flowers appeared to be clearly distinct from all the other organs analysed in terms of their high VOC
concentration. Qualitatively detected VOCs clearly separated all the vegetative organs from flowers
and berries, then the roots and rachis from other vegetative organs and flowers from berries, which
confirms the specialization in volatile production among different organs.
Our real-time RT-PCR results revealed that the majority of TPS genes analysed exhibited detectable
transcripts in all the organs investigated, while only some were found to be expressed specifically in
one or just a few organs. In most cases, we found that the known products of the in vitro assay of VvTPS
enzymes corresponded well to the terpenes found in the organs in which the encoding gene was
expressed, as in the case of (E)-b-caryophyllene synthases, a-terpineol synthase and a-farnesene synthase.
In addition, we found groups of homologous TPS genes, such as (E)-b-caryophyllene and b-ocimene
synthases, expressed distinctively in the various tissues. This thus confirmed the subfunctionalization
events and a specialization on the basis of the organs in which they are mostly expressed
Lombardesca
No full textLa prima citazione della 'Lombardesca nera' è di Basso (1992) che l'ha identificata in Garfagnana (Lucca), mentre il sinonimo 'Corvara nera' è stato accertato più recentemente sempre in garfagnana (D'Onofrio et a., 2015).La Corvara/Lombardesca nera non corrisponde a nessuno dei genotipi caricati nei vari Vitis database e appare essere presente esclusivamente in Garfagnana (D'Onofrio et al., 2015).L’uva è dolce e ha elevata acidità totale. Appare essere un vitigno di 3° epoca
Canina bianca
No full textLa prima citazione di 'Gagnaccia bianca' è recente: è stata segnalata per la prima volta da Basso (1992) che l'ha identificata in Garfagnana.
Il successivo progetto di Recupero e Caratterizzazione del germoplasma viticolo della Garfagana (respnsabile scientifico Prof. Claudio D'Onofrio, Università di Pisa) ha permesso di verificare la 'Canina bianca' e 'Cagnaccia bianca' diffusi in Garfagnana sono sinonimi, come pure la 'Petognana bianca' diffusa nel medesimo areale (D'Onofrio et al., 2015).
Moreover, this variety do not match to the 'Canina bianca' identified in Sicilia e non è un sinonimo della 'Vernaccia di Oristano' come talvolta suggerito (D'Onofrio et al., 2015)
Ruolo del deossinivalenolo nella competizione per la paglia da parte di funghi filamentosi
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