1,720,983 research outputs found
Immunità passiva colostrale nell'agnello neonato: correlazioni tra l'attività enzimatica di GGT, ALP, AST, ALT, LDH e PsChE e la concentrazione di immunoglobuline sieriche
No full textil lavoro, condotto su 47 agnelli di razza sarda ha permesso di dimostrare che lo stato del trasferimento passivo colossale nell'agnello neonato può essere predetto con successo mediante il dosaggio dell'attività dell'enzima GGT. Altri enzimi, quali l'ALP, l'AST, l'ALT, la LDH e la PcChE, non si sono invece dimostrati utili in tal senso
Evaluation of assay procedures for prediction of passive transfer status in lambs
No full textObjective
To compare 4 assay procedures for prediction of passive transfer status in lambs.
Animals
Thirty-one 1-day-old Sardinian lambs.
Procedure
Serum IgG concentration was determined by use of single radial immunodiffusion. The following were determined: serum total protein concentration as measured by refractometry (ie, refractometry serum total protein concentration), serum total protein concentration as determined by the biuret method (ie, biuret method serum total protein concentration), serum gamma-globulin concentration as determined by serum protein electrophoresis, and serum gamma-glutamyltransferase (GGT) activity as measured by spectrophotometry. Accuracy of these assays for estimation of serum IgG concentration in 1- day-old lambs was established by use of linear regression analysis.
Results
Refractometry serum total protein concentration, biuret method serum total protein concentration, and serum gamma-globulin concentration were closely and linearly correlated with serum IgG concentration. The natural logarithm (ln) of serum GGT activity was closely and linearly correlated with serum IgG concentration (ln). Refractometry serum total protein concentration, biuret method serum total protein concentration, and gamma-globulin concentration accounted for approximately 85%, 91%, and 95% of the variation in serum IgG concentration, respectively. Serum GGT activity (ln) accounted for approximately 92% of the variation in serum IgG concentration (ln).
Conclusions and Clinical Relevance
For prediction of passive transfer status in 1-day-old lambs, serum GGT activity or biuret method serum total protein concentration determination will allow for passive transfer monitoring program development. Immediate refractometry serum total protein concentration determination is beneficial in making timely management and treatment decisions. Serum gamma-globulin concentration determination can be used as a confirmatory test
Evaluation of serum enzyme activities as predictors of passive transfer status in lambs
No full textObjective-To determine the associations between serum IgG concentration and serum activities of gamma-glutamyltransferase (GGT), alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and pseudocholinesterase for the potential use of these serum enzymes as predictors of passive transfer status in neonatal lambs. Design-Prospective observational study. Animals-47 Sardinian lambs from birth to 2 days old. Procedure-Serum enzyme activities were measured by use of commercially available kits and a clinical biochemical analyzer. Serum IgG concentration was determined by single radial immunodiffusion. Associations between serum IgG concentration and the activity of each serum enzyme were established by use of regression analysis. Results-A significant correlation was detected between serum IgG concentration and serum GGT activity in 1- and 2-day-old lambs. Minimal correlations were detected between serum IgG concentration and serum alkaline phosphatase activity in 1-day-old lambs and serum pseudocholinesterase activity in 1- and 2-day-old lambs. No significant associations were detected between serum IgG concentration and serum activities of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase. A multiple linear regression model was accurate for the estimation of the natural logarithm of serum IgG concentration as a function of the natural logarithm of serum GGT activity and of the age of lambs at the time of sampling (adjusted R-2 = 0.89). This model was then used to calculate the serum GGT activity equivalent to various serum IgG concentrations for 1- and 2-day-old lambs. Conclusions and Clinical Relevance-Results suggested that passive transfer status in neonatal lambs can be successfully predicted by measurement of serum GGT activity but not by measurement of the other enzymes tested.[...
Effect of passive transfer status on preweaning growth performance in dairy goat kids
No full textObjective-To evaluate the effect of passive transfer status (determined from measurements of serum IgG concentration at 24 hours after parturition [slgG-24]) on preweaning growth performance in dairy goat kids. Design-Prospective observational study. Animals-20 healthy nursing dairy doe kids in a natural nonintensive breeding environment. Procedures-For each kid, slgG-24 was measured. Body weight was measured at birth and at the time of weaning 30 days (ie, 29 to 31 days) after birth; average daily gain from birth to day 30 and weight at day 30 were used as measures of preweaning growth performance. Regression analysis was used to evaluate associations between slgG-24 and measures of preweaning growth performance. Results-Mean ±SD slgG-24 was 31.7 ±10.3 mg/mL Mean body weights at birth and weaning were 4.105 ±0.981 kg (9.031 ±2.158 Ib) and 9.310 ±2.554 kg (20.482 ±5.619 Ib), respectively; average daily gain was 0.174 ±0.072 kg/d (0.383 ±0.158 Ib/d). No significant association was detected between slgG-24 and birth weight. However, slgG-24 was significantly associated with average daily gain (R2 = 0.48) and weight at day 30 (R2 = 0.56). Each increase in slgG-24 of 1 mg/mL was associated with an increase in average daily gain of 0.005 kg/d (0.011 Ib/d) and an increase in weight at day 30 of 0.185 kg (0.407 Ib). Conclusions and Clinical Relevance-Results indicated that passive transfer status (determined as slgG-24) was a significant source of variation in preweaning growth performance in dairy doe kids reared in this nonintensive breeding environment
EFFECT OF PASSIVE TRANSFER STATUS ON PREWEANING GROWTH PERFORMANCE IN DAIRY GOAT KIDS
No full textOBJECTIVE: To evaluate the effect of passive transfer status (determined from measurements of serum IgG concentration at 24 hours after parturition [sIgG-24]) on preweaning growth performance in dairy goat kids.
DESIGN: Prospective observational study.
ANIMALS: 20 healthy nursing dairy doe kids in a natural nonintensive breeding environment.
PROCEDURES: For each kid, sIgG-24 was measured. Body weight was measured at birth and at the time of weaning 30 days (ie, 29 to 31 days) after birth; average daily gain from birth to day 30 and weight at day 30 were used as measures of preweaning growth performance. Regression analysis was used to evaluate associations between sIgG-24 and measures of preweaning growth performance.
RESULTS: Mean +/- SD sIgG-24 was 31.7 +/- 10.3 mg/mL. Mean body weights at birth and weaning were 4.105 +/- 0.981 kg (9.031 +/- 2.158 lb) and 9.310 +/- 2.554 kg (20.482 +/- 5.619 lb), respectively; average daily gain was 0.174 +/- 0.072 kg/d (0.383 +/- 0.158 lb/d). No significant association was detected between sIgG-24 and birth weight. However, sIgG-24 was significantly associated with average daily gain (R(2) = 0.48) and weight at day 30 (R(2) = 0.56). Each increase in sIgG-24 of 1 mg/mL was associated with an increase in average daily gain of 0.005 kg/d (0.011 lb/d) and an increase in weight at day 30 of 0.185 kg (0.407 lb).
CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that passive transfer status (determined as sIgG-24) was a significant source of variation in preweaning growth performance in dairy doe kids reared in this nonintensive breeding environmen
Evaluation of assay procedures for prediction of passive transfer status in lambs
No full textObjective-To compare 4 assay procedures for prediction of passive transfer status in lambs. Animals-Thirty-one 1-day-old Sardinian lambs. Procedure-Serum IgG concentration was determined by use of single radial immunodiffusion, The following were determined: serum total protein concentration as measured by refractometry (ie, refractometry serum total protein concentration), serum total protein concentration as determined by the biuret method (ie, biuret method serum total protein concentration), serum gamma-globulin concentration as determined by serum protein electrophoresis, and serum gamma-glutamyltransferase (GGT) activity as measured by spectrophotometry. Accuracy of these assays for estimation of serum IgG concentration in 1-day-old lambs was established by use of linear regression analysis. Results-Refractometry serum total protein concentration, biuret method serum total protein concentration, and serum gamma-globulin concentration were closely and linearly correlated with serum IgG concentration. The natural logarithm (In) of serum GGT activity was closely and linearly correlated with serum IgG concentration (In) Refractometry serum total protein concentration, biuret method serum total protein concentration, and gamma-globulin concentration accounted for approximately 85%, 91%, and 95% of the variation in serum IgG concentration, respectively. Serum GGT activity (In) accounted for approximately 92% of the variation in serum IgG concentration (In). Conclusions and Clinical Relevance-For prediction of passive transfer status in 1-day-old lambs, serum GGT activity or biuret method serum total protein concentration determination will allow for passive transfer monitoring program development. Immediate refractometry serum total protein concentration determination is beneficial in making timely management and treatment decisions. Serum gamma-globulin concentration determination can be used as a confirmatory test.[...
Effect of passive transfer status on preweaning growth performance in dairy lambs
No full textObjective
To evaluate the effect of passive transfer status, determined by measuring serum IgG concentration 24 hours after parturition, on preweaning growth performance in dairy lambs.
Design
Prospective observational study.
Animals
20 healthy Sardinian dairy lambs.
Procedures
Serum IgG concentration was measured 24 hours after birth. Body weight was measured at birth and at the time of weaning 28 days (ie, 27 to 29 days) after birth. Mean daily gain from birth to day 28 and day-28 weight were used as measures of preweaning growth performance. Regression analysis was used to evaluate associations between serum IgG concentration 24 hours after birth and measures of preweaning growth performance.
Results
Mean +/- SD serum IgG concentration 24 hours after birth was 24.6 +/- 17.5 mg/mL. Mean body weights at birth and weaning were 2,696 +/- 937 g and 9,253 +/- 2,116 g, respectively, and mean daily gain was 234 +/- 63 g/d. No significant association was detected between serum IgG concentration 24 hours after birth and birth weight. However, serum IgG concentration 24 hours after birth was significantly associated with mean daily gain (R2 = 0.26) and day-28 weight (R2 = 0.25). Each 1 mg/mL increase in serum IgG concentration 24 hours after birth was associated with a 1.8 g/d increase in mean daily gain and a 60.8 g increase in day-28 weight.
Conclusions and Clinical Relevance
Results indicated that passive transfer status, determined as serum IgG concentration 24 hours after birth, was a significant source of variation in preweaning growth performance in dairy lambs
Attività dell'enzima gamma-glutamiltransferasi nel latte di pecore sane e con mastite subclinica
No full textL'indagine, condotta su 285 campioni di latte prelevati da pecore con mammelle apparentemente sane, dimostra che la determinazione dell'e'attività dell'enzima gammaglutamiltrasferasi nel latte rappresenta un indicatore di scarsa utilità del numero di cellule somatiche stante la scarsa correlazione e evidenziata (P=0,0011 R2=0,05). pertanto questo enzima non è un marker utile per la valutazione della sanità della mammella nella specie ovina, almeno per le patologie subclinich
Attività dell’enzima gamma-glutamiltransferasi nel latte di pecore sane e con mastite subclinica
No full text
- …
