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Specific binding of nicergoline on alpha1-like adrenoreceptor in the rat retina
No full textSystemic treatment with nicergoline, an ergoline derivative showing al-antagonist properties, causes vasodilatation in the eye without apparent untoward cardiovascular effects. In the present work we investigated the ability of nicergoline to inhibit the binding of radiolabelled prazosin in the rat retina and cortex.
We found that nicergoline inhibited [H-3]prazosin binding in both tissues, being more potent than unlabelled prazosin in the retinal tissue. The competition curves of the ergoline derivative were well fitted by a one-site model in the cortical tissue, with an IC50 (concentration of the drugs needed to inhibit the binding of labelled prazosin by 50%) of 2.54 x 10(-8)M, and by a two-site model in the retinal tissue, with IC50 values of 7.08 x 10(-12) M and 1.82 x 10(-5)M. 2-(2,6 dimetoxyphenoxyethyl) aminomethyl-1,4-benzodioxane hydrochloride (WB4101) and phentolamine, selective ligands for the high-affinity binding site for prazosin, in particular the alpha(1A)-site, fully inhibited prazosin binding in the cortex but only partially inhibited prazosin binding in the retina, being less potent in this tissue than either nicergoline or prazosin.
Our results suggest that a binding component of alpha(1)-adrenoreceptors is expressed to a lesser extent in the retina than the cortex, leading to a reduced response of the retinal tissue to prazosin, and more particularly to WB4101 and phentolamine. The selective binding of the nicergoline on this retinal adrenoreceptor may explain the peculiar efficacy of the drug in ocular pathophysiology
Sentinel Node Detection of Endometrial Cancer, the Advantages of a New Needle for Injection of Tracers
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Valutazione di alcuni ormoni stress-correlati in cavalli prima e dopo stordimento con pistola a proiettile captivo
No full textThis work aimed to survey the slaughtering-linked plasma modifications of some stress-related hormones in horses subject to butcher standardized procedures. The blood samples of 12 males horses, ageing from 3 to 5 years, have been collected before slaughtering, in lairage, and after stunning by captive bolt gun, during exsanguination. The plasma levels of beta-endorphin, cortisol, epinephrine and norepinephrine have been assayed
by EIA. The results indicate that beta-endorphin did not significantly increase after stunning, while cortisol (P<0.05) and catecholamines (P<0.001) significantly increased. The ratio between the plasma level of norepinephrine and epinephrine decreases significantly (P<0.001) during the time considered for observation underlining a greater involvement of adrenalmedulla in the stress response. Moreover these results suggest that the beta-endorphin release,
under stress, could be different from ACTH release
Sentinel node detection of endometrial cancer, the advantages of a new needle for injection of tracers
No full textInfection by rhinoestrus spp. larvae in equids in southern Italy : A new myiasis for the old continent
No full textTwo hundred and twelve native horses, were examined in 2 slaughterhouses in the Apulia region from January to November 2003, and 120 native horses and two donkeys in one slaughterhouse in Sicily from January to October 2003. Thirteen of 212 and five of 120 horses examined from Apulia and Sicily, respectively, were infested by nasal bot fly larvae. Both donkeys examined were positive. Overall, 213 larvae (L1,12 and 13), were collected from the infected animals. Larvae (L3) were identified on the basis ofperitreme structures and arrangement of the spines on the dorsal surface of the third segment. Some L3 collected from Apulia and Sicily presented morphological characteristics of both Rhinoestrus usbekistanicus and Rhinoestrus purpureus. The presence of mixed features on the L3 of Rhinoestrus collected from the same animals may be explained by the existence of a single species of Rhinoestrus presenting different morphotypes or of two species of Rhinoestrus (R. usbekistanicus and R. purpureus,) which are both present in southern Italy
Larve di Rihoestrus spp. negli equidi in Puglia e Sicilia: una nuova Miasi per il vecchio continente
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