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Use of single and tandem plasmids for GMO quantification in maize and soybean by SybrGreen Real-time PCR
Aggiornamento sulle problematiche da virus delle colture orticole in serra.
An update on viruses infecting greenhouse vegetable crops
in Italy
Updates on some viruses infecting cucurbits and tomato
crops grown in greenhouses are briefly outlined. Evolutionary
aspects of WMV and ZYMV concerning the emergence
of necrogenic strains and seed transmissibility of the two
viruses are discussed as well as the unexpected detection
of TYLCV in cucumber, melon, squash and watermelon.
Since TYLCV infections in cucurbits are symptomless, the
risk of latent infections for tomato crops is highlighted. As
for tomato crops, for the first time substantial evidence is
provided that reassortants constitute a consistent part of the
natural population of TSWV. Finally, eco-epidemiological
consequences of seed transmission of PZSV and PepMV are
discussed envisaging that phytosanitary restrictions must be
applied to seed to limit the spread of harmful plant viruses
Synergies and antagonisms in virus interactions
Metagenomic surveys and data from next generation sequencing revealed that mixed infections among plant viruses are probably a rule rather than an exception in natural pathosystems. The documented cases may range from synergism to antagonism, which may depend from the spatiotemporal order of arrival of the viruses on the host and upon the host itself. In synergistic interactions, the measurable differences in replication, phenotypic and cytopathological changes, cellular tropism, within host movement, and transmission rate of one of the two viruses or both are increased. Conversely, a decrease in replication, or inhibition of one or more of the above functions by one virus against the other, leads to an antagonistic interaction. Viruses may interact directly and by transcomplementation of defective functions or indirectly, through responses mediated by the host like the defense mechanism based on RNA silencing. Outcomes of these interactions can be applied to the risk assessment of transgenic crops expressing viral proteins, or cross-protected crops for the identification of potential hazards. Prior to experimental evidence, mathematical models may help in forecasting challenges deriving from the great variety of pathways of synergistic and antagonistic interactions. Actually, it seems that such predictions do not receive sufficient credit in the framework of agriculture
Synergism in plant–virus interactions: a case study of CMV and PVY in mixed infection in tomato
Viral Misadventures During Movement In Grafted Vegetables: An Alternative Way To Produce Virus-Resistant Crops
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