1,720,992 research outputs found
A live mammalian cells electroporation array for on-chip immunofluorescence
No full textThe detection of intracellular proteins in vitro is commonly realized with immunofluorescence techniques, through which antibodies or markers are delivered into fixed cells and recognize specific proteins. Many innovative techniques, however, avoid cells fixation by chemical compounds and, among the others, electroporation is widely used. Here we demonstrate that in situ electroporation on thin film SiO2 capacitive microelectrodes can be realized with high efficiency to deliver fluorescent markers and antibodies into mammalian cell lines and primary neuronal cells to detect intracellular proteins, like actin. The results presented in this work open the way to the use of this technique for the detection of potentially any target protein, even through subsequent electroporations
High Resolution Cortical Imaging Using Electrolyte-(Metal)-Oxide-Semiconductor Field Effect Transistors
No full textA MATLAB Based Toolbox for Processing and Analysis of Multichannel, Multisource Cortical Local Field Potentials
No full textAn Automated Method to Determine Angular Preferentiality using LFPs Recorded from Rat Barrel Cortex by Brain-Chip Interface under Mechanical Whisker Stimulation
No full text
A High Resolution Bi-Directional Communication through a Brain-Chip Interface.
No full textExisting brain-machine interfacing techniques allow
either high precision recordings from one or a few single
neurons, or low spatial resolution recordings with a sparse
sampling within the networks. Through our app-roach an
efficient simultaneous bidirectional communication to the
brain is realized using capacitively coupled recording and
stimulation sites arranged in a large 2D multi-transistor array
(MTA) with 1000 elements, integrated to a planar chip at high
resolution (10μm pitch and below). The aim of the present
work is to evaluate the reliability of a simple-generation silicon
micro-device in recording neuronal signals from rat brain.
Simultaneous recording of signals using this chip from the
somatosensory cortex (S1) of living rat, are compared to
standard in vivo recordings with a glass micropipette. We show
that the two types of signals are identical, indicating the
possibility to record signals at the same time from different
sites and to perform a real-time electrical imaging of the brain
cortex in vivo
A MATLAB Based Tool for Cortical Layer Activation Order Detection through Latency Calculation in Local Field Potentials Recorded from Rat Barrel Cortex by Brain-Chip Interface
No full textAn Automated Method to Remove Artifacts Induced by Microstimulation in Local Field Potentials Recorded from Rat Somatosensory Cortex
No full textProcessing of Neuronal Signals Recorded by Brain-Chip Interface from Surface of the S1 Brain Cortex
No full textWith the rapidly growing means of neuronal signal recording, it is very important to perform suitable processing to infer meaningful conclusions. The signals recorded from surface of the S1 brain cortex using brain-chip interface are noisy and prone to stimulus artifact, thus noise characterization and artifact removal are required to obtain a clean signal. Here we present an easy way to process these signals using techniques for artifact removal and noise characterization to clean the neuronal recordings and assess the quality of the recorded signals, thus the recording device
- …
