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miRNA biomarkers for the diagnosis of Duchenne Muscular Dystrophy progression, for monitoring therapeutic interventions and as therapeutics
No full textBrevetto Internazionale PCT/EP2010/05708
The DAG1 and DAG2 Dof zinc finger genes influence with opposite effects germination of Arabidopsis seeds
No full textThe Dof proteins are a family of plant transcription factors ubiquitously present in plants, but absent from yeast and animals. These proteins are characterised by a strikingly conserved (Dof) domain containing a single zinc finger and a downstream basic region. All Dof proteins bind similar DNA target sequences with a CTTT core. By means of a reverse genetics approach, we demonstrated that the Dof genes DAG1 and DAG2 are both involved, but with opposite roles, in the control of Arabidopsis seed dormancy and germination. In particular, inactivation of DAG1 considerably increases the germination capability of the seeds, while mutation of DAG2 results in seeds with a substantially lower germination potential than the wild type. All the physical and hormonal stimuli known to promote germination are almost superflous for dag1 seeds, whereas for dag2 seeds they are more essential than for the WT. The DAG1 and DAG2 proteins share an identical Dof domain and a high degree (77%) of aminoacid identity outside the domain. Furthermore, the DAG1 and DAG2 genes have identical expression patterns, limited to the vascular system of the mother plant but not of the embryo. This is in good agreement with the maternal effect of both the dag1 and the dag2 mutations. In addition, DAG1 is epistatic over DAG2. These evidences allow to formulate a model whereby the zinc finger proteins DAG1 and DAG2 act on a maternal switch that controls seed germination, possibly by regulating the same gene(s)
The microRNA transcription machinery: analysis of costitutive and tissue-specific microRNAs
No full text
DAG1 and DAG2: two Arabidopsis transcription factors that play a maternal role in controlling seed germination”
No full textThe Arabidopsis Dof transcription factor DAG1 is involved in phyB- and GA-mediated seeds germination
No full textDAG1 AND DAG2, TWO HIGHLY RELATED TRANSCRIPTION FACTORS INVOLVED IN SEED GERMINATION, ACT ON THE SAME TARGET GENES WITH OPPOSITE EFFECTS
No full textBARCELONA, SPAI
The Dof protein DAG1 mediates PIL5 activity on seed germination by negatively regulating GA biosynthetic gene AtGA3ox1
No full textP>We have previously shown that inactivation of the gene encoding the Arabidopsis thaliana transcription factor DOF AFFECTING GERMINATION 1 (DAG1) renders seed germination more sensitive to both phytochrome B (phyB) and gibberellins (GA). dag1 mutant seeds require less red (R) light fluence and a lower GA concentration than WT to germinate. Here, we show that inactivation of the gene PHYTOCHROME INTERACTING FACTOR 3-LIKE 5 (PIL5) results in down-regulation of DAG1. Inactivation of PIL5 in the dag1 mutant background further increased the germination potential of dag1 mutant seeds, supporting the suggestion that DAG1 is under the positive control of PIL5. Germination of dag1phyB seeds showed a reduced requirement of gibberellins as compared with phyB mutant seeds, both in the presence and in the absence of GA biosynthesis. Furthermore, the GA biosynthetic gene AtGA3ox1 is upregulated in dag1 seeds as compared with the WT, and DAG1 actually binds to the AtGA3ox1 promoter, as shown by chromatin immunoprecipitation experiments. Expression analysis at different time points confirms that AtGA3ox1 is directly regulated by DAG1, while suggesting that DAG1 is not a direct regulatory target of PIL5. Our data indicate that in the phyB pathway leading to seed germination, DAG1 negatively regulates GA biosynthesis and suggest that DAG1 acts downstream of PIL5. In addition, the analysis of hypocotyls of dag1 and phyB mutant plantlets, of plantlets overexpressing phyB in the dag1 mutant, as well as of dag1phyB double mutant suggests that DAG1 may act as a negative regulatory element downstream of phyB also in hypocotyl elongation
DAG1 and DAG2: two Arabidopsis transcription factors that play a maternal role in controlling seed germination
No full text“DAG1 e DAG2: due proteine “materne” coinvolte nella germinazione dei semi di Arabidopsis thaliana”
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