1,720,973 research outputs found
Superovulatory response to gonadotrophin FSH/LH treatment and effect of progestin supplement to recipients on survival of transferred vitrified embryos in goats
No full textTwo experiments were carried out in goats to evaluate the effects of the FSH/LH ratio during treatment on ovarian response and embryo production (experiment 1) and the efficiency of progestin supplementation on pregnancy and the survival of vitrified embryos (experiment 2). In experiment 1, 30 goats were synchronized and allocated to 2 groups (n = 15) corresponding to the following superovulatory treatments with p-FSH (250 IU, over 3 days) having different doses of purified FSH and LH: (group A) control, FSH/LH ratio of 1, kept constant during treatment; (group B) FSH/LH ratio of 2 and daily FSH/LH ratio of 5.0:1.0:0.3 for the first, second, and third days of treatment, respectively. Ovarian response and embryo production were assessed 7.5 days after estrus. In experiment 2, 46 vitrified blastocysts from p-FSH-superovulated donors were transferred to 26 recipients (2 blastocysts per goat) 7.5 days after estrus. The recipients were synchronized with donors and allocated to 2 experimental groups (n = 13). Group C received progestin supplement as fluorgestone acetate (FGA) inserted into the vagina at the time of embryo transfer, replaced with a new one 16 days later, and maintained until the 45th day of pregnancy; group D, no treatment (control). Pregnancy was diagnosed by transrectal ultrasound scanning on Days 30 and 45 after estrus and followed to term. The results indicated that the increase in FSH/LH ratio from 1 to 2 with decreasing daily FSH/LH (treatment B) did not improve the superovulatory response. Superovulatory treatment A (control) advanced (P < 0.05) the onset of estrus and showed a higher ovulation rate compared to group B (14.9 vs. 10.9; P < 0.05). Fertilization rate, embryo yield, and mean number of transferable embryos in group A (7.5) were higher (P < 0.05) than those in group B (3.2). Recipient goats receiving progestin supplementation (group C) showed a higher (P < 0.05) pregnancy rate and embryo survival (kids born per embryos transferred; 69.3% and 73.1%) than the controls (group D; 23.3% and 19.2%). In conclusion, regimen A with FSH/LH ratio of 1 kept constant during the treatment gave the best ovarian response and embryo production. The progestin supplementation as FGA-pessary administered at embryo transfer time to the 45th day of pregnancy improved the pregnancy rate, kidding rate, and embryo survival of transferred vitrified embryos. Intravaginal progestin supplement has the potential to reduce the incidence of pregnancy losses during early pregnancy
Fatty acid composition and hedonic ratings of meat from light lambs of leccese breed in relation to slaughter age
Full text linkTwenty lambs from Leccese local breed were used to investigate the effect of two slaughter ages (45 vs 60 d) on fatty acid composition and hedonic ratings of meat. The lambs, born as singles in spring, were subdivided into two groups (n. 10) corresponding to the slaughter ages of 45 and 60 d. The animals received their mother's milk and a supplementation of hay and concentrate from 30 d to slaughter. The increase to 60 d of slaughter age resulted in higher proportion of lauric acid (C12:0; P<0.05), pentadecanoic acid (C15:0; P<0.01) and conjugated linoleic acid (CLA; P<0.05), and lower proportion of stearic acid (C18:0; P<0.05) and linoleic acid (C20:3 n-6; P<0.05). Using a none-point hedonic scale, consumer test showed that meat from lambs slaughtered at 60 d received a higher hedonic scores (P < 0.01) as well as higher scores for tenderness (P < 0.05), flavour (P < 0.05), and juiciness (P < 0.001) than meat from lambs slaughtered at 45 d. These findings might be useful to characterise lamb meat of local origin in relation to its nutritional traits and market perspectives connected to consumer acceptability
Udder characteristics and effects of pulsation rate on milking machine efficiency in donkeys
No full textVery little is known about the udder characteristics, partitioning of milk in the mammary gland and efficiency of machine milking in donkeys. This study aimed to evaluate the characteristics of the udder and teats, milk yield in relation to pulsation rates (90, 120 and 150 cycles/min), milk partitioning in the mammary gland, composition of the spontaneously removed and residual milk fractions and milking efficiency. Forty-one healthy Martina Franca jennies in the third month of lactation and routinely milked twice daily were used in three studies. Udder characteristics were evaluated by direct measurements and ultrasonographic scanning. Residual milk was obtained by milking after an oxytocin administration (40 IU i.m.). The prevalent shapes were 'bowl' for udders and 'conical' for teats. After milking the udder characteristics decreased within a range from-11·6% (udder depth) to-25·7% (diameter of teat at the base). The internal structures of the udder resulted as several pockets of ducts empting directly into the teat. The pulsation rate of 120 cycles/min improved (P<0·05) the milk yield in comparison to the 90 and 150 cycles/min, reduced the residual milk fraction, thus improved (P<0·05) milking efficiency. Residual milk composition had higher (P<0·05) fat content and somatic cell count than the spontaneously removed milk fraction. The udders revealed several pockets of ducts empting into the teat instead of a single cisternal cavity and showed a certain compliance. The use of 120 cycles/min pulsation rate improved milking efficiency
Effects of microbial transglutaminase levels on donkey cheese production
No full textMicrobial transglutaminase (MTGase) is an enzyme widely used in the dairy sector to improve the functional properties of protein-based products via the formation of a network between protein molecules. The aim of this study involving cheese from the milk of donkeys was to evaluate the effects of treatment with MTGase at the concentrations of 0 (control), 5, 8 and 10 U/g milk protein on the cheese-making process parameters, as well as the physical and chemical characteristics of the resulting cheese. MTGase influenced the time of gel formation from rennet addition (P < 0.05), with a delay at the two highest concentrations, accompanied by a lower (P < 0.01) pH of cheese and the lowest (P < 0.01) loss in cheese weight at 24 h of storage. The highest gel viscosity (P < 0.01) was observed at the highest concentration of the enzyme, reaching the value of 70 mPa⋅sec after 60 min. The chemical composition and color of the cheeses were not significantly affected by the inclusion of MTGase, regardless of the enzyme concentration. These findings may be of relevance in adapting the cheese-making process and might help in the design of new dairy products from donkey's mil
PrP genotype frequencies and risk evaluation for scrapie in dairy sheep breeds from southern Italy
No full textConcerns regarding scrapie in sheep breeding have increased in the last few decades. The present study was carried out in dairy sheep breeds from southern Italy. In order to find breeding animals resistant to scrapie, the PrP genes of 1,205 animals from entire flocks of dairy native Apulian Leccese and Altamurana breeds, and Sicilian Comisana breed, were analysed for polymorphisms at codons 136, 154, and 171 related to scrapie resistance/susceptibility. The Altamurana breed was considered as two populations (Alt-Cav and Alt-Cra-Zoe), based on presumed cross-breeding. A total of five alleles and ten different genotypes were found. The ARQ allele was predominant for all breeds followed by ARR, the most resistant allele to scrapie, which was highly prevalent in Comisana (50%) and in native Alt-Cav (42.4%). The VRQ allele, associated with the highest susceptibility to scrapie, was detected at not negligeable levels in allocthonous Comisana (3.5%), at a low frequency (0.2%) in native Leccese and Alt-Cra-Zoe, while it was absent in Alt-Cav. The frequencies of PrP genotypes with a very low susceptibility risk to scrapie (R1) was higher in Comisana and Alt-Cav. The most susceptible genotype, ARQ/VRQ, was found only in Comisana. Within the Altamurana breed, there were notable differences between Alt-Cav and Alt-Cra-Zoe sheep. The Alt-Cav was characterised by the absence of VRQ and AHQ alleles and by the higher frequency of the ARR/ARR genotype (18.7%). Breeding programs, mainly in endangered breeds such as Altamurana, should be conducted gradually, combining resistance to scrapie, maintenance of genetic variability, and production
Potential Use of Wheat Straw, Grape Pomace, Olive Mill Wastewater and Cheese Whey in Mixed Formulations for Silage Production
Full text linkTwo experiments were conducted to investigate the chemical and fermentative characteristics of by-product-mixed silages consisting of wheat straw (WS), grape pomace (GP), olive mill wastewater (OMWW) and cheese whey (CW) at 7, 30 and 90 days. The silage formulations were based on a ratio of 60% solids (WS + GP) and 40% liquids (CW + OMWW), with the addition of water (W) where necessary to achieve 40% of liquids. In experiment 1, the effects of the inclusion of GP or CW in a mixture of WS and OMWW were studied according to two silage formulations: SIL-A, WS40% + OMWW5% + GP20% + W35%; SIL-B, WS60% + OMWW5% + CW35%. In experiment 2, the effects of two levels of CW and the inclusion of OMWW in mixed silages based on WS, GP, and CW were studied according to four silage formulations: SIL-C, WS40% + GP20% + CW20% + W20%; SIL-D, WS40% + GP20% + CW20% + OMWW5% + W15%; SIL-E, WS40% + GP20% + CW35% + W5%; SIL-F, WS40% + GP20% + CW35% + OMWW5%. In experiment 1, the silage formulation affected the chemical composition showing a greater (p p p > 0.05) in the chemical characteristics of the silages were found. In both of the experiments, the chemical composition and total phenol content did not change (p > 0.05) during the ensiling period. Fermentative characteristics were not affected (p > 0.05) by the by-product combination nor the ensiling period and proved to be adequate for good-quality silages. The Flieg’s scores at D30 and D90 were greater than a 100 score in all the experimental silages, leading to the conclusion that WS, GP, OMWW and CW can be effective for producing silage
Fatty acid composition of light lamb meat from Leccese and Comisana dairy breeds as affected by slaughter age
Full text linkForty lambs of two Italian dairy breeds were used to study the effects of slaughter age and breed on meat fatty acid composition. Lambs were subdivided into four groups (n. 10) according to a factorial scheme of two breeds (Leccese and Comisana). ×. two slaughter ages (45 and 60 days). The lambs were fed maternal milk supplemented with hay and concentrate from the 30th day to the slaughter. Leccese lambs at 45 days exhibited a FA profile more compatible to nutritional requirements for human health. They displayed a lower SFA proportion, a higher UFA/SFA and MUFA/SFA ratios than Comisana. The delay of slaughtering age at 60 days improved FA composition in Comisana lambs which had lower SFA content, AI and TI indexes and higher UFA/SFA and MUFA/SFA ratios and n-3 PUFA content than in Leccese. In both the breeds, the slaughter age at 60 days improved the CLA content
Oxidative stress, aging, antioxidant supplementation and their impact on human health: An overview
No full textAging is characterized by a progressive loss of tissue and organ function due to genetic and environmental factors, nutrition, and lifestyle. Oxidative stress is one the most important mechanisms of cellular senescence and increased frailty, resulting in several age-linked, noncommunicable diseases. Contributing events include genomic instability, telomere shortening, epigenetic mechanisms, reduced proteome homeostasis, altered stemcell function, defective intercellular communication, progressive deregulation of nutrient sensing, mitochondrial dysfunction, and metabolic unbalance. These complex events and their interplay can be modulated by dietary habits and the ageing process, acting as potential measures of primary and secondary prevention. Promising nutritional approaches include the Mediterranean diet, the intake of dietary antioxidants, and the restriction of caloric intake. A comprehensive understanding of the ageing processes should promote new biomarkers of risk or diagnosis, but also beneficial treatments oriented to increase lifespan
Evaluation of genetic variability within PrP genotyped sheep of endangered Italian Altamurana breed
No full textIn the last few decades, there has been increased awareness of preservation and exploitation of endangered breeds for the maintenance of biodiversity, as well as the concern for diseases in sheep breeding.This study was carried out in native endangered Altamurana dairy sheep breed from Southern Italy. The Altamurana breed was considered as two populations (Alt-Cav and Alt-Cra-Zoe), based on presumed cross-breed and remarkable differences in the PrP genotypes frequencies. The genetic diversity between the two Altamurana populations (Alt-Cav and Alt-Cra-Zoe) was evaluated in comparison to three Italian dairy breeds through fourteen microsatellite markers. Both measures of genetic distance and the population structure analysis highlighted that the Alt-Cav and Alt-Cra-Zoe sheep have a particular genetic background. The estimated fixation index (FST) and the genetic Nei's distances among the populations showed a higher level of genetic differentiation for Alt-Cav than Alt-Cra-Zoe. The Bayesian clustering analysis implemented in the STRUCTURE software showed clear and distinct clusters for the two Altamurana populations, confirming the hypothesis of Alt-Cav as a genetic group well differentiated from Alt-Cra-Zoe. Alt-Cav likely can be considered as belonging to the original strain of the Altamurana breed. This findings may be used to assist the programme for conservation and selection of scrapie resistance genotypes in endangered Altamurana sheep breed
Effect of STAT5A7Eco81I gene polymorphism on milk yield in Garganica goat breed
No full textSignal transducers and activators of transcription (STATs) are latent cytoplasmic transcription factors. They
belong to a 7-member family (STAT1-4, STAT5A, STAT5B, and STAT6) playing a prominent role as mediators
of many hormone and cytokines within target cells. STAT5 is known as the main mediator of growth hormone
action on target genes; it plays a key-role as mediator of prolactin signalling and can activate transcription
of milk protein genes in response to prolactin. A single STAT5 gene was identified in sheep, subsequently
two forms, STAT5 and STAT5B have been identified. The STAT5A gene has been mapped to chromosome
19 in goat. Many polymorphisms at STAT5A gene has been investigated in bovine species as candidate
markers affecting quantitative traits. To date, only few SNPs at STAT5A gene were detected in goat species
and few studies have been conducted. The aims of the present study were to investigate the STAT5A/Eco81I
polymorphism in a sample of Garganica goats, an Italian autochthonous breed, and to investigate the possible
association between this polymorphism and milk production. Most of Italian goats breeds are represented
by small populations with peculiar characteristics and variable productive traits. Garganica breed showed
an exceptional ability to adapt to difficult environments and to use poor pasture. This breed originates in the
Gargano promontory as a result of a crossbreeding with west European goats. Nowadays, the Garganica goat
breed is included in the list of Italian endangered breeds drawn up by the Italian Department for Environment,
Food, and Rural Affairs. In the investigated Garganica population, only two out of three possible genotypes
were identified, moreover the association between milk yield and different genotypes was investigated
- …
