1,720,996 research outputs found
Synapsins Are Downstream Players of the BDNF-Mediated Axonal Growth
No full textSynapsins (Syns) are synaptic vesicle-associated phosphoproteins involved in neuronal development and neurotransmitter release. While Syns are implicated in the regulation of brain-derived neurotrophic factor (BDNF)-induced neurotransmitter release, their role in the BDNF developmental effects has not been fully elucidated. By using primary cortical neurons from Syn I knockout (KO) and Syn I/II/III KO mice, we studied the effects of BDNF and nerve growth factor (NGF) on axonal growth. While NGF had similar effects in all genotypes, BDNF induced significant differences in Syn KO axonal outgrowth compared to wild type (WT), an effect that was rescued by the re-expression of Syn I. Moreover, the significant increase of axonal branching induced by BDNF in WT neurons was not detectable in Syn KO neurons. The expression analysis of BDNF receptors in Syn KO neurons revealed a significant decrease of the full length TrkB receptor and an increase in the levels of the truncated TrkB.t1 isoform and p75NTR associated with a marked reduction of the BDNF-induced MAPK/Erk activation. By using the Trk inhibitor K252a, we demonstrated that these differences in BDNF effects were dependent on a TrkB/p75NTR imbalance. The data indicate that Syn I plays a pivotal role in the BDNF signal transduction during axonal growth
In vivo effects of phosphodiesterase inhibition on basal cGMP levels in the pre-frontal cortex, hippocampus and cerebellum of freely moving rats.
No full text‐Aspartate as an amino acid neurotransmitter: mechanisms of the depolarization‐induced release from cerebrocortical synaptosomes
No full text
Synapsin II mutations associated with autism or epilepsy: effects on neuronal development and synaptic vesicle cycling
No full text
Activity-dependent structural plasticity of cerebellar climbing fibers and its functional consequences
No full text
Role of Synapsin II mutations associated with autism or epilepsy in neuronal development and synaptic vesicle cycling
No full text
- …
