1,721,010 research outputs found
Antioxidant properties of minimally processed (ready-to-eat) Italian Cichorium genus salads
No full textThe modern lifestyle is characterized by a continuous increase in the demand of ready-to-eat fresh food products subjected to minimal processes prior marketing. Attention is drawn towards the effect of this processing on the antioxidant/antiradical properties of two typical Italian salads belonging to Cichorium genus. Four chemical assays (anti-peroxyl radical activity, DPPH• assay, metal chelating ability assay, and reducing power assay) were used for evaluating these properties. The results indicated that the anti-peroxyl radical activity, the anti-DPPH radical activity, the metal chelating ability, and the reducing power were significantly higher in fresh samples as compared to 8-days stored samples (p < 0.001). Minimal processing applied to vegetables further affected the tested properties causing a decrease over time from 24h to 8d of storage at 4°C. Overall, the results presented in this study showed that any minimal processing applied to ready-to-eat vegetable affects the antioxidant/antiradical properties determining a decrease in the values registered in the four chemical assays
Direct analysis of urinary trans,trans-muconic acid by coupled column liquid chromatography and spectrophotometric UV detection. Method applicability to human urine
No full textThe method developed can be used for direct analysis of the benzene metabolite, tt-muconic acid in urine samples collected from benzene exposed workers
Improved coupled column liquid chromatographic method for high speed direct analysis of urinary trans,trans-muconic acid as a biomarker of exposure to benzene
No full textA coupled column liquid gaschromatographic method for high speed analysis of the urinary ring-opened benzene metabolite, tt-muconic acid is described.
This analytical procedure could be valuably applied to occupational medicine for biological monitoring of benzene exposure
Systematic Toxicological Analysis by Gas Chromatography-Continuos Scan Mass Spectrometry
No full textEffects of sorbic acid ingestion on urinary trans, trans-muconic acid excretion in rats exposed to low levels of benzene
No full textIngestion of sorbic acid, a widely used food additive, significantly affected the urinary levels of the benzene metabolite tt-muconic acid in rats exposed to 1 ppm benzene for 6 hours
Combining DoE and MASE: a winning strategy for the isolation of natural bioactive compounds from plant materials
No full textThe successes achieved in pursuing a nature-aided drug discovery (NADD) program are many and well-known, but it is still considered a second-order approach. Biomass extraction is a fundamental and critical step in the NADD process and often requires a high volume of usually organic and not eco-compatible solvents and a prolonged time. Optimization of such procedures could drastically decrease the costs required for the NADD process, also considering waste management. For this reason, many extraction techniques have been developed, among which one of the most diffused is microwave assisted solvent extraction (MASE). The MASE procedure is well suited for use in the drug discovery phase from natural sources. Still, there are several factors to consider, and the one-factor-at-a-time (OFAT) approach risks limiting the advantages the technique provides. The way to make it truly green is to couple MASE with DoE, even if this winning combination is limited. Consistently, we analyze the 10-year literature (2013–2022), reporting a critical discussion about DoE applied to set up MASE protocols for the extraction of metabolites (both performed with traditional solvents and with ionic and eutectic solvents) and essential oils
GC-O and GC-MS analytical profile of a fragrant compound prepared by microwave assisted synthesis
No full textDetermination of purine and pyrimidine bases, nucleosides, and nucleotides 5'-monophosphate by hydrophilic liquid interaction chromatography (HILIC) and UV detection
No full textPhenolic compounds in red Chicorium intybus vegetable
No full textPolyphenols are biologically active compounds widely distributed in the plant kingdom and so present in plant-derived foods and intrinsic components of human diets. Polyphenols have been shown to possess a range of biological activities, that are consistent with them contributing to the protection afforded by a diet rich in fruit and vegetables against degenerative diseases, inflammatory process, and aging, and modulating the immunity system.
Cichorium intybus with its different varieties is a genus rich in polpyphenols; in fact, it is known to contain a number of hydroxycinnamic acid derivatives and flavonoids, with anthocyanins characterizing the var. silvestre, but the qualitative evaluation of polyphenolic fraction of red chicories, commonly used as food, is far to be completed. As the degree of hydroxylation of polyphenols is important in determining the biological activity and the degree and type of glycosylation are important in determining the ability of these compounds to be adsorbed in humans, the aim of this work was to investigate red chicory variety largely consumed in Italian diet, characterizing the different isomeric forms of polyphenols. The investigation was carried out using HPLC-DAD-ESI-MSn. The obtained results indicated the presence of a variety of phenolic acids in their different isomeric forms, flavonols (kaempferol and quercetin derivatives), and anthocyanidins glycosilated with one or more sugar moieties
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