1,533 research outputs found
Il giudizio etico sui comportamenti imprenditoriali: verso una modellizzazione secondo l’Approccio Sistemico Vitale (ASV)
No full textATTENZIONE: edizione riveduta ed aggiornata
BARILE S., GATTI M., PROIETTI L., Il giudizio etico sui comportamenti imprenditoriali: verso una modellizzazione secondo l’Approccio Sistemico Vitale (ASV), in Sergio Barile (a cura di), L’impresa come sistema. Contributi sull'Approccio Sistemico Vitale (ASV), Giappichelli, Torino, 2006
Capitolo CON attribuzioni ai singoli AutoriWARNING: revised and updated edition BARREL S., CATS M., L. Proietti, Ethical judgment on entrepreneurial behavior: towards a modeling according to the Systemic Approach Vitale (ASV), in Sergio Barile (ed.), The enterprise as a system. Contributions on the approach Systemic Vitale (ASV), Giappichelli, Turin, 2006 Chapter WITH allocations to individual Author
Il giudizio etico sui comportamenti imprenditoriali: verso una modellizzazione secondo l’Approccio Sistemico Vitale (ASV)
No full textBARILE S., GATTI M., PROIETTI L., Il giudizio etico sui comportamenti imprenditoriali: verso una modellizzazione secondo l’Approccio Sistemico Vitale (ASV), in Sergio Barile (a cura di), L’impresa come sistema. Contributi sull'Approccio Sistemico Vitale (ASV), Giappichelli, Torino, 2006
Capitolo senza attribuzioni ai singoli AutoriBARREL S., CATS M., L. Proietti, Ethical judgment on entrepreneurial behavior: towards a modeling according to the Systemic Approach Vitale (ASV), in Sergio Barile (ed.), The firm as a system. Contributions on the approach Systemic Vitale (ASV), Giappichelli, Turin, 2006 Chapter without attribution to individual author
Mantaining homeostasis of flavin cofactors: importance for cellular bioenergetics and redox balance
No full text
Profili identitari e metafore per capire le potenzialità educative dei Social Network
No full textAnalizzare i Social Network (SN) può aiutare a capire le loro potenzialità a supporto dell’apprendimento. Partendo da un quadro teorico socio costruttivista e utilizzando concettualizzazioni relative agli artefatti culturali, ai posizionamenti identitari e alle metafore, questa ricerca si propone di delineare dei profili degli utenti dei SN allo scopo di individuare modalità d’uso in contesti educativi. Un apposito questionario, composto da 28 domande che indagano quattro aree tematiche (informazioni personali, orientamento e preferenze, modalità di utilizzo, metafore), è stato compilato da 327 partecipanti, la maggior parte di età compresa tra i 16 e i 27 anni. Dall’analisi dei dati emergono tre profili (Interdipendenza / Socialità, Dipendenza, Individualità) che permettono di superare i tre livelli elaborati da Wartofsky, prospettando l’emergere di un nuovo quarto livello, caratterizzato da artefatti che permettono di costruire mondi personali entro mondi collettivi astratti e simbolici. Infine, si propongono alcuni principi guida per un’eventuale progettazione di attività educative supportate da artefatti di quarto livello
Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions
No full textRiboflavin, or vitamin B2, is the precursor of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), essential redox (and sometimes non-redox) cofactors of a large number of flavoenzymes involved in energetic metabolism, protein folding, apoptosis, chromatin remodeling, and a number of other cell regulatory processes.The cellular and subcellular steady-state concentrations of flavin cofactors, which are available for flavoprotein biogenesis and assembly, depend on carrier-mediated transport processes and on coordinated synthesizing/destroying enzymatic activities, catalyzed by enzymes whose catalytic and structural properties are still matter of investigation.Alteration of flavin homeostasis has been recently correlated to human pathological conditions, such as neuromuscular disorders and cancer, and therefore we propose here protocols useful to detect metabolic processes involved in FAD forming and destroying.Our protocols exploit the chemical-structural differences between riboflavin, FMN , and FAD , which are responsible for differences in the spectroscopic properties (mainly fluorescence) of the two cofactors (FMN and FAD); therefore, in our opinion, when applicable measurements of fluorescence changes in continuo represent the elective techniques to follow FAD synthesis and degradation. Thus, after procedures able to calibrate flavin concentrations (Subheading 3.1), we describe simple continuous and rapid procedures, based on the peculiar optical properties of free flavins, useful to determine the rate of cofactor metabolism catalyzed by either recombinant enzymes or natural enzymes present in cellular lysates/subfractions (Subheading 3.2).Fluorescence properties of free flavins can also be useful in analytical determinations of the three molecular flavin forms, based on HPLC separation, with a quite high sensitivity. Assaying at different incubation times the molecular composition of the reaction mixture is a discontinuous experimental approach to measure the rate of FAD synthesis/degradation catalyzed by cell lysates or recombinant FAD synthase (Subheading 3.3). Continuous and discontinuous approaches can, when necessary, be performed in parallel
Thiamine uptake by isolated rat liver mitochondria - submitochondrial thiamine distribution
No full textPurification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD/Protein Monomer Ratio by UV-Vis Spectra
No full textHere we describe a protocol for a one-step purification of a soluble form of human FAD synthase (isoform 2; hFADS2), overexpressed as a 6-His-tagged fusion protein in Escherichia coli, with a yield of about 15 mg from 1 L of transformed bacterial culture.Following a desalting procedure, the protein is obtained in its FAD-bound form (about 0.8 molecules of FAD per 1 protein monomer). A simple method is also proposed here, for the rapid estimation of the [FAD ]/[protein monomer] ratio, starting from the typical flavoprotein spectrum of the purified protein fraction.The procedure described gives the protein at a quite high grade of purity (about 95%) and in its bifunctional (2.7.7.2/3.6.1.18) enzymatically active form, useful for further kinetical and molecular characterization
Alteration of Flavin Cofactor Homeostasis in Human Neuromuscular Pathologies
No full textThe aim of this short review chapter is to provide a brief summary of the relevance of riboflavin (Rf or vitamin B2) and its derived cofactors flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) for human neuromuscular bioenergetics.Therefore, as a completion of this book we would like to summarize what kind of human pathologies could derive from genetic disturbances of Rf transport, flavin cofactor synthesis and delivery to nascent apoflavoproteins, as well as by alteration of vitamin recycling during protein turnover
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