1,720,976 research outputs found
Molecular Characterization of Italian Heavy Draught Horse (IHDH) using mitochondrial DNA and microsatellite markers
No full textGenetic Chatacterization of Italian Heavy Draught Horse (IHDH) breed using mirosatellite markers
No full textGenetic variability of Italian Heavy Draught Horse
No full textThis study aimed to analyze the genetic variability of the Italian Heavy Draught Horse (IHDH) breed using a panel of 23 microsatellite markers. We also compared the population structure of the IHDH to other two unrelated breeds (Italian Haflinger, IH and Quarter Horse, QH). The IHDH showed a genetic variability comparable with other European heavy draught horse breeds and with the IH and QH breeds analyzed. Clustering analyses using a posterior Bayesian approach clearly differentiated the three breeds; it also showed a fragmentation of the IHDH in three subpopulations that need to be further investigated. These findings are an indicator of the present situation of the IHDH and will contribute to the conservation and implementation of the selection programme for this breed
Temporal variation in genetic diversity and population structure of Burlina cattle breed
Full text linkWe analysed the temporal variation of inbreeding, genetic variability and population structure in the Burlina (BUR) cattle breed. A total of 279 individuals were chosen for the analysis representing a period of 19 years (1991-2010) and analysed using 24 microsatellite markers. A total of 235 alleles were detected in the population with a mean of 9.79 +/- 3.91 alleles per locus. In the 19-year period, a stable pattern in the mean number of alleles was found. The mean observed heterozygosity was 0.63 and it was slightly lower than the expected in all birth year groups. Neither an increase nor a decrease in heterozygosity and inbreeding estimates were detected over the years, with the exception of the F-IS index which was close to zero in two birth year groups: 2001-2002 and 2006. Absence of bottleneck events was proved and structure analysis revealed an increase in breed complexity over the years and a clear differentiation with the Italian Holstein Friesian cattle breed. Molecular markers were successfully applied in the monitoring of the genetic variability of BUR thus enabling the planning and the application of strategies for the in situ conservation of genetic resources and the improving of breed identity
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes
No full textA SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s rRNA gene was amplified using interspecific conserved primer and sequenced. The sequences were aligned and analyzed for the presence of SNPs; three SNPs (MerSNP1, MerSNP7 and MerSNP9) were identified and selected to allow discrimination between the four species. Aplotypes were TCC, CCC, CAT and CAC for Pacific cod, Atlantic cod, Haddock and Ling respectively. Confirmation of results was achieved by sequencing 16s rRNA gene fragments of 16 G. morhua, 7 G. macrocephalus, 15 M. aeglefinus and 5 M. molva samples collected at different fish catching campaign. Nucleotide sequence of 16s rRNA mitochondial gene has been shown to be a useful tool to allow rapid reliable and fully automatable for discrimination of 4 economically important species in fisheries industry
I "public concern" e legislazione comunitaria, nazionale e regionale sugli organismi geneticamente modificati
No full textGenetic analysis reveals Roe deer (Capreolus capreolus) population structure in North-Eastern Italian Alps
No full textThis preliminary study described the genetic variability and analysed the population structure of 119 roe deer (Capreolus capreolus) sampled over the provinces of Belluno and Trento, in the north-eastern Italian Alps, using 11 microsatellite markers. The panel of microsatellites was highly informative, and the whole population was subdivided into 2 distinct sub populations. The observed ecological population sub-units did not coincide with the administrative subdivision (the provinces borders) that are now in use for management. The results of this work confirm that molecular genetic approaches may provide useful indication for roe deer management in that area
I public concern e legislazione comunitaria, nazionale e regionale sugli organismi geneticamente modificati
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