76,504 research outputs found
Le nuove forme del dialetto.
No full textIl volume affronta l'argomento proposto da diverse prospettive teoriche e metodologiche, mettendo a confronto studiosi di diverse discipline e di diverse sedi universitarie. Temi affrontati e nome degli autori: Prefazione (G. Marcato); Dialetti fra tradizione e innovazione (P. D'Achille, G. Ruffino, E. Assenza, A.G. Mocciaro, L. Chillà, S.C. Trovato, P. Del Puente, A. Cantisani, A. Dantonio, T. Giammaria, L. Passacantando, N. Arigoni, I. D'Agostino); Instabilità e variazione dialettale (M. Maiden, E. Triantafillis, M. Da Tos, R. Lai, L. Marano, A. Zorzan); Dialetto, mondo letterario, media (P. Koch, G. Alfieri, A. Dettori, S. Bressan, G. Brian, M. Giuliano, L. Girardi, A.M. Boccafurni, M. Romano); Nuovi spazi per il dialetto: la rete e i giovani (F. Lurà, C. Zoli, A. Viviani, M. Apostolico, R. Sardo, M.T. Vigolo, P. Barbierato, E. Mastantuoni, I. Tempesta, A. Schena); Contatti linguistici e riassestamento del repertorio (M. Tessarolo, L. Gaddi, R. Sottile, E. Capitummino, M. Burgio, A. Batinti, A. Lamanna, E. Golovko, B. Moss, V.M. Frosi, G. Marcato
Cell proliferation and apoptosis in the pathogenesis of oesophagogastric lesions in pigs
No full textRecent studies have stressed the importance of epithelial hyperproliferation in the pathogenesis of early lesions (parakeratotic hyperkeratosis) of the porcine gastric pars oesophagea (PO). In this study, immunohistochemical staining with Ki67 (clone MIB 1) and AgNOR proteins silver staining were used to evaluate, by means of image analysis, cell proliferation in normal and parakeratotic (parakeratotic hyperkeratosis) epithelia of the PO. Apoptotic activity was also assessed with the TUNEL assay and compared with cell proliferative parameters. Early lesions of the PO were characterised by a significant increase in epithelial proliferative activity while there was no difference in the apoptotic activity between normal and parakeratotic epithelia. Our data confirm the hyperproliferative nature of epithelial changes preceding degeneration and erosion/ulcer of the PO and suggest that an underlying feature of gastric ulcers in pigs is an imbalance between cell proliferation and programmed cell death. © 2000 Harcourt Publishers Ltd
Detection of proliferating cell nuclear antigen (PCNA) in canine and feline mammary tumours
No full textQuantitation of immunohistochemical staining of the proliferating cell nuclear antigen (PCNA, clone PC10) by image analysis was performed on benign and malignant mammary tumours of dogs and cats. Scoring of the slides was carried out by image analysis to assess the percentage of labelled nuclei (expressed as a ratio of areas). Either the strongly labelled nuclei (SP-PCNA index), or all of the stained nuclei (TP-PCNA index) were counted as positive to determine the growth fraction and its correlation with the histopathological classification and nuclear grade (degree of nuclear differentiation, considered a morphological correlate of tumour aggressiveness). A significant difference in the values of PCNA indices was seen between benign and malignant growths (P<0·0001, dog; P<0·05, cat). Neither of the PCNA indices showed correlation with nuclear grade in dogs (P=0·14 for SP-PCNA index and P=0·31 for TP-PCNA index) or cats (P=0·09 for SP-PCNA index and P=0·07 for TP-PCNA index). A significant difference in the number of mitoses, expressed as mitotic index, was seen between benign and malignant growths in the dog (P<0·01) but not in the cat (P=0·078). Good correlation of mitotic index with nuclear grade was revealed in canine malignant growths (P<0·05), but in feline malignant tumours such correlation ({itP<0·05) was shown only when the values of intermediate plus typical forms were compared with the data for atypical forms. It is concluded that quantitation of PCNA-positive nuclear area by image analysis provides an objective method for assessing proliferative activity in benign and malignant mammary tumours of dogs and cats. © 1995 Academic Press Limited
Proliferative activity assessed by anti-PCNA and Ki67 monoclonal antibodies in canine testicular tumours
No full textThe recent availability of monoclonal antibodies raised against cell cycle nuclear antigens makes possible, by means of immunohistochemical techniques, an easy and quick method of evaluating tumour kinetic activity, in addition to older methods such as measurement of the mitotic index. Some of these antibodies can be used on formalin-fixed paraffin wax-embedded samples, thus allowing the use of archival material. In the present study the proliferative activity of testicular tumours of the dog (seminomas and Sertoli and Leydig cell tumours) was investigated with two monoclonal antibodies to proliferating cell nuclear antigen (PCNA) clone PC10, and Ki67 clone MIB1. The former recognizes a formalin-resistant epitope of PCNA, and MIB1 the same antigen as Ki67 in formalin-fixed, paraffin wax-embedded sections after incubation in a microwave oven. Three parameters of proliferative activity were considered: PCNA and Ki67 indices (percentage of nuclear area positive to PCNA and to Ki67), and mitotic index (number of mitoses per 1000 cells). The PCNA index and Ki67 index revealed a good correlation in linear regression analysis (P<0·001) as did the mitotic index (P<0·01). None of the parameters considered revealed a significant difference in proliferative activity of the three types of tumour (P<0·05—Spearman test), but in both seminomas and Sertoli cell tumours the progression from tubular to diffuse pattern paralleled an increase in growth fraction. It is interesting that some seminomas of the diffuse type, often considered on histological grounds to be the most malignant, showed the highest values of the above-mentioned parameters. © 1994, Academic Press Limited. All rights reserved
Giant Cells in anaplastic mammary carcinoma of the dog and cat
No full textFour uncommon anaplastic mammary carcinomas containing numerous giant cells are described in three dogs and one cat. The giant cells of all cases were studied by means of monoclonal and polyclonal antibodies to detect epithelial (carcinoembryonic antigen and keratin) and mesenchymal (vimentin, lysozyme and S-100 protein) differentiation. Most of them proved to have an epithelial immunophenotype. Ultrastructurally, scattered bundles of tonofilaments but no lysosome-like bodies could be detected. One tumour had an additional, different type of giant cell, which had a benign multinucleated osteoclast-like appearance, gave positive staining for acid phosphatase, had a histiocytic-stromal immunohistochemical pattern, and was, ultrastructurally, multinucleate with irregular folds and no evidence of tonofilaments. In one case some giant cells had an epithelial immunophenotype and others a stromal immunophenotype, even though their histological and ultrastructural features were the same. In the least histologically differentiated tumour the giant cells presented a coexpression of intermediate filaments. This supported the theory that there might be a stem cell origin for most canine mammary tumours. © 1993, Academic Press Limited. All rights reserved
Tra lingua e dialetto
No full textTemi affrontati e nome degli autori dei saggi: Lingua e dialetto naturalmente coesistenti. Questioni di metodo (E.Triantafillis, G.Marcato, N.De Blasi); Tra matrice linguistica e repertorio (L. Corrà, P. Avena, A. Schena, T. Giammaria, L. Passacantando, L. Marano, I. Scarpello, L. Amenta, C. Piazza, F. Modena, S. Widlak, M.Montes Lopez, S. Rossi, M. Berizzi); Lingua, dialetto e linguaggi creativi (C.Giovanardi, G. Sulis, D. Pescarini, A. Viviani, A.M. Boccafurni, P. Maturi, M. Tassi, N. Arigoni); Locuzioni, toponimi, lessici dialettali ( P. D'Achille, P. Barbierato, M.T.Vigolo, A. Cascone, M. Burgio, A. Batinti, A. Lamanna, R. Sottile, F. Avolio, A. Stefinlongo, M. Castiglione, I. Tempesta, R. Fresu, A. Colonna Romano, V. Lospennato, E. Assenza); Considerazioni fonetiche, morfofonologiche, sintattiche (S.C. Trovato, L. Chillà, P. Dal Puente, R. Ferri, P. Curcio, A. Cantisani, F.V. Russo, E. Mastantuoni, F. Finco, P. Chinellato, N. Penello, M. Da Tos, L. Rigobianco, E. Algozino)
Dialetto. Uso, funzioni, forma.
No full textIl volume fa il punto sulla questione affrontando l'argomento da diverse angolature, mettendo a confronto studiosi di diverse discipline e diverse prospettive teoriche e metodologiche. lTemi trattati e nome degli autori: Riflettendo sulla forma, tra modello e uso (L. Giannelli, M. Magnanini, R.P. Abbamonte, E. Assenza, G. Soriani, M. da Tos, A. De Cassan, F. Modena, D. Pescarini, L. Rigobianco, c. Zanini, M. Di Salvo, F. Finco, J. Garzonio, B. Pacini, L. Passacantando, N. Penello, G. Vitolo); Dialetto e italiano nella ristrutturazione del costume e del codice (M. Tessarolo, P. D'Achille, P. Barbierato, M.T. Vigolo, M. Burgio, A.M. Boccafurni, R. Sottile, I. tempesta, D. De Salvatore, A. Bitonti); Sinergie tra italiano e dilaletto, dalle narrazioni orali, agli scritti informali, all'uso letterario, alla comunicazione in rete (L. Vanelli, V. Lospennato, G. Paternostro, A.G. Mocciaro, S.C. Trovato, C. Grosso, D. Giordano, R. Sardo, G. Sulis, T. Giammaria, A. Centi Pizzutilli, G. Scarano); "Valore" di una varietà linguistica e sue funzioni all'interno del repertorio (G. Alfonzetti, A. Batinti, A. Lamanna, L. Amenta, M. Castiglione, M. Gargiulo, M. Tassi, E. Algozino, A. Schena, L. Giacchi, L. Melchior, G. Pizzolato, F. Benucci); L'italiano nel mondo, tra lingua e dialetti (J. Al Shuaibi, S. Widlak, M. Boureux, V.M. Frosi)
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