6,521 research outputs found
Nuclear localization and signalling activity of inositol lipids
No full textIt has been recently shown that also the nucleus is a site for both synthesis and hydrolysis of the phosphorylated forms of phosphatidylinositol. Among the enzymes of the cycle we and others have demonstrated that phospholipase C specific for inositol lipids (PLC) is one of the main steps of the inositol lipid cycle. The PLC family and namely the type raised a noticeable interest since, because of their common COOH-terminus, it has been shown a nuclear localisation in addition to that at the plasma membrane. The autonomous intranuclear inositide cycle is endowed, besides the PLC, with conventional lipid kinases and phosphatidylinositol 3 kinase (PI 3-kinase) which plays an important role in granulocytic differentiation. The combination of morphology and molecular biology gave us the opportunity to localise the sites inside the nucleus where the cycle takes place and to understand the physiological significance of such a subcellular compartmentalisation both during cell growth and differentiation
Chromatin Phospholipids in Normal and Chronic Lymphocytic Leukemia Lymphocytes
No full textCertain phospholipids are associated with the nonhistone chromosomal proteins extracted from normal B- and chronic lymphocytic leukemia lymphocytes. The ratio of phospholipids to nonhistone chromosomal proteins was constant with the different methods used for isolating nuclei and extracting the chromatin, although the various methods allowed a different recovery of total lipids from chromatin. Three phospholipids were extractable from the nonhistone protein fraction, but their respective ratios varied in chronic lymphocytic leukemia compared to normal B-lymphocytes. The most significant variation concerns the reduction of sphingomyelin content in leukemic lymphocytes, since this prospholipid in vitro affects both DNA stability and transcription
Lipid-F1 nucleohistone interactions
No full textHigh concentrations of phospholipids determine destabilization of F1 histone-DNA complex at the weight ratios, histone:DNA, 0.8:1 and 1:1, but low concentrations cause only negligible destabilization. Cholesterol at high weight ratios has little effect on nucleohistone stability. Only linolenic acid of the fatty acids used reproduces similar changes in the thermal stability of F1 histone-DNA complex as phospholipids. The type of interaction of phospholipids with the F1 histone-DNA complex is analyzed, and the involvement of phospholipids in DNA replication in vivo is discussed
Effect of phospholipid vesicles on the activity of terminal transferase
No full textThe terminaI transferase activity is modified in the presence of
Iipid vesicles. A deep inhibitory effect takes pIace with phosphati-
dylserine and phosphatidylinositol, while some stimulation is pre-
sent with sphingomyelin and almost no effect has been detected with
phosphatidylethanolamine vesicles. These effects seem to be related
to the charge properties of the Iipid membranes.
A possible involvement of phospholipids in the mechanism of action
of the terminaI transferase is suggested
Lipid--DNA interactions. II. Phospholipids, cholesterol, glycerophosphorylcholine, spingosine and fatty acids.
No full textHigh concentrations of lecitin, phosphadylethanolamine, phosphatidyl-serine, cholesterol and lauric acid have a destabilizing effect on the DNA helix. On the contrary, lower concentrations of the same lipids produce stabilization on DNA. The stabilizing and destabilizing effects with sphingomyelin components can be mainly attributed to sphingosine and lignocerin acid components, whwreas glycerophosphorycholine probably has a minimal effect. The saturated long-chain fatty acids were capable of having a moderate destabilizing effect on DNA; however, the unsaturated fatty acids were found to be generally more stabilizing in nature. The type of bonding within the lipid-DNA interaction has been investigated and the existence of a lipid-DNA complex in vivo has been considered
Incarnare la realtà. La dicotomia cinema-teatro nella teoria pasoliniana dell'attore.
No full textIl saggio fa il punto sulla teoria dell'attore di Pasolini, confrontando la teoria teatrale con quella cinematografica
Prefazione
No full textLa prefazione fa il punto sull'integrazione degli studi sull'industria all'interno della ricerca relativa al cinema e all'audiovisivo
Dall'educazione al cinema alla digital media literacy: storia e prospettive.
No full textIl saggio fa il punto sulla trasformazione della cosiddetta "educazione al cinema" nel più ampio orizzonte della media literacy
Non è roba per femminucce. Media, aging e sessualità.
No full textIl saggio fa il punto sulla rappresentazione dell'invecchiamento femminile nella cultura mediale italiana contemporanea
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