1,721,139 research outputs found
Sepsis : diagnostic methods and protocols
No full textThis volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely recognized that inappropriate treatment is associated with a dramatic increase in mortality, especially within the first hours, therefore clinical and microbiological diagnosis are of pivotal importance in the management of septic patients. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls
Analysis of the Enteric Microbiome: First Tentative Steps Towards a Comprehensive Work-up of Prostate Cancer?
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Molecular signatures of hepatitis C virus (HCV)-induced type II mixed cryoglobulinemia (MCII)
Full text linkThe role of hepatitis C virus (HCV) infection in the induction of type II mixed
cryoglobulinemia (MCII) and the possible establishment of related lymphoproliferative
disorders, such as B-cell non-Hodgkin lymphoma (B-NHL), is well ascertained.
However, the molecular pathways involved and the factors predisposing to the
development of these HCV-related extrahepatic complications deserve further
consideration and clarification. To date, several host- and virus-related factors have been
implicated in the progression to MCII, such as the virus-induced expansion of selected
subsets of B-cell clones expressing discrete immunoglobulin variable (IgV) gene
subfamilies, the involvement of complement factors and the specific role of some HCV
proteins. In this review, we will analyze the host and viral factors taking part in the
development of MCII in order to give a general outlook of the molecular mechanisms
implicated
Perspectives for the utilization of neutralizing human monoclonal antibodies as anti-HCV drugs
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Structural and antigenic definition of hepatitis C virus E2 glycoprotein epitopes targeted by monoclonal antibodies
Full text linkHepatitis C virus (HCV) is the major cause of chronic liver disease as well as the major indication for liver transplantation worldwide. Current standard of care is not completely effective, not administrable in grafted patients, and burdened by several side effects. This incomplete effectiveness is mainly due to the high propensity of the virus to continually mutate under the selective pressure exerted by the host immune response as well as currently administered antiviral drugs. The E2 envelope surface glycoprotein of HCV (HCV/E2) is the main target of the host humoral immune response and for this reason one of the major variable viral proteins. However, broadly cross-neutralizing monoclonal antibodies (mAbs) directed against HCV/E2 represent a promising tool for the study of virus-host interplay as well as for the development of effective prophylactic and therapeutic approaches. In the last few years many anti-HCV/E2 mAbs have been evaluated in preclinical and clinical trials as possible candidate antivirals, particularly for administration in pre- and post-transplant settings. In this review we summarize the antigenic and structural characteristics of HCV/E2 determined through the use of anti-HCV/E2 mAbs, which, given the absence of a crystal structure of this glycoprotein, represent currently the best tool available
SARS-CoV-2 infection despite high levels of vaccine-induced anti-Receptor-Binding-Domain antibodies: a study on 1110 health-care professionals from a northern Italian university hospital
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