1,721,127 research outputs found
EMILIN1 role in tumor growth after enzymatic degradation
Full text linkEMILIN1 is a ECM glycoprotein involved in several cellular processes. In particular, EMILIN1 is involved in elastogenesis processes and in the maintenance of lymphatic vessels structures. EMILIN1 presents several regulatory properties exercised through its EMI domain that is located at N-terminal region and is able to regulate homeostasis of blood pressure, and its gC1q domain located at C-terminal region. In particular, gC1q domain is involved in the regulation of both cell adhesion and cell proliferation through the binding with α4β1 integrin. EMILIN1-/- mice present an increase of tumoral cell proliferation; this is due to the loss of the interaction between gC1q domain-α4β1 integrin, that determines the activation of the MAPK pathway, resulting in upregulation of cell proliferation. Studies previously published in our laboratory demonstrated that neutrophil elastase, released by neutrophils present in tumor microenvironment, is able to degrade EMILIN1 resulting in the loss of its regulatory functions.
Other authors have proposed EMILIN1 a possible substrate of several matrix metalloproteinases: matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase 14 (MT1-MMP). We demonstrated that among these MMPs only MT1-MMP shows a weak proteolytic activity on EMILIN1. Moreover, we observed that MT1-MMP was not able to impair EMILIN1 functions. On the contrary we observe that the digestion of EMILIN1 with neutrophil elastase was able to impair EMILIN1 tumor suppressor role. At this step, we wanted to analyze the capability of neutrophil elastase to degrade the gC1q domain. We digested the gC1q domain with several proteases and we observed that among the tested proteases only neutrophil elastase was able to degrade the gC1q domain and to impair its functionality. Thus, we wanted to pinpoint the neutrophil elastase cleavage site on gC1q domain, in order to generate a mutant of gC1q domain resistant to neutrophil elastase cleavage. We consulted several peptidase database that contained predicted neutrophile elastase cleavage sites, on these basis we generated several gC1q mutants. Among these mutants that generated, we found that the mutant R914W, in which aminoacid arginine was substituted with aminoacid tryptophan, was resistant to neutrophil elastase cleavage. Functional adhesion and proliferation assays confirmed the capability of R914 mutant to maintain its properties after neutrophil elastase treatment
Stable isotope geochemistry and structural elements of the Sabina region (Central Apennines, Italy)
No full textStable isotope geochemistry and examination of meso- and microstructures show that strain conditions differed through the course of tectonic history. Carbon and oxygen isotope analyses of the calcite-filled extensional fractures, the sigmoidal veins present between stylolitic cleavage surfaces, and fault plane surfaces with differing motion, demonstrate those different geneses. -from Author
Emotion in motion: a kinesemiotics analysis of character interpretation through dance discourse
No full textBallet is a very popular form of art that attracts audiences of fans all over the world, especially when traditional classical ballet performances are released by established ballet companies. However, very few people really understand how dancers communicate through the interaction between choreographed body-structures and space. As a consequence, new choreographies that, unlike the great classical crowd-pleasers, are not based on well-known stories or characters tend to attract much smaller audiences. Drawing on the principle of Projection, which informs the Functional Grammar of Dance (Maiorani 2017, 2021) and the new interdisciplinary research area of Kinesemiotics (Maiorani 2021), this article will provide an example of comparative analysis of dance sequences that will show how, through different choices of Projections, two different dancers actually express different meanings and emotions even if dancing the same role on the same score. The article will also discuss the implications of such an analysis and its possible automated development in terms of potential for data collection and archival.</p
Emotion in motion: a kinesemiotics analysis of character interpretation through dance discourse
No full textBallet is a very popular form of art that attracts audiences of fans all over the world, especially when traditional classical ballet performances are released by established ballet companies. However, very few people really understand how dancers communicate through the interaction between choreographed body-structures and space. As a consequence, new choreographies that, unlike the great classical crowd-pleasers, are not based on well-known stories or characters tend to attract much smaller audiences. Drawing on the principle of Projection, which informs the Functional Grammar of Dance (Maiorani 2017, 2021) and the new interdisciplinary research area of Kinesemiotics (Maiorani 2021), this article will provide an example of comparative analysis of dance sequences that will show how, through different choices of Projections, two different dancers actually express different meanings and emotions even if dancing the same role on the same score. The article will also discuss the implications of such an analysis and its possible automated development in terms of potential for data collection and archival.</p
He, C and S isotopes in subaerial and submarine fumaroles from the caldera of Phlaegrean Fields, Italy
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Personalized Treatment of Periodontitis in a Patient with McArdle’s Disease: The Benefits from Probiotics
No full textIntroduction. McArdle’s disease is a severe glycogen storage disease characterized by intolerance to exercise; patients have a syndrome of muscle intolerance to stress, associated with myalgia, cramps, fatigue, and muscle weakness. Periodontal disease is a multifactorial pathology of the supporting tissues of the teeth: one of the main factors is the formation of bacterial biofilm; its control favors the prevention and the maintenance of good health of the oral cavity; and some systemic pathologies can worsen the periodontal disease and hinder its therapy. This case report concerns a woman with McArdle’s disease diagnosed with periodontal disease. Material and Methods. A 54-year-old female patient with McArdle’s disease has been diagnosed with Stage 3 generalized periodontitis, Grade B. At the baseline, the patient had 82 pockets with probing pocket depth (PPD) equal to or greater than 4 mm. The patient was instructed in the correct methods of oral hygiene and was advised toothpaste and mouthwash based on probiotics; subsequently, a debridement was performed to remove etiological factors using Dental-Biofilm Detection Topographic Technique (D-BioTECH). Results. After 60 days, the number of pockets was reduced from 82 to 14 overall with PPD≥4 mm and from 50 to 2 pockets with PPD≥5 mm. Full mouth bleeding score (FMBS) increased from 48% to 15% and full mouth plaque score (FMPS) from 73% to 15%. Conclusions. In this case, the use of a correct brushing method combined with the D-BioTECH has reduced the disease state, with the use of probiotics at home to restore and maintain a healthy oral microbiome
Rethink Architecture. Riqualificazione di Valle Giulia, sede della facoltà di Architettura della Sapienza
No full textCome rendere comprensibile il ruolo ed il valore che il progetto degli spazi interni ha per la vita di ciascuno? Ci è sembrato che un tema efficace potesse essere quello che chiama gli studenti a ri-pensare gli interni della propria facoltà; spazi in cui hanno vissuto per anni valutandone, nel bene e nel male, le caratteristiche fisico-spaziali e le ricadute che queste hanno avuto nella loro quotidianità.
Il progetto Rethink Architecture sviluppa una riflessione sugli spazi odierni della Facoltà di Valle Giulia ipotizzandone un rinnovamento a partire dal sistema degli spazi distributivi. Tramite la progettazione di nuovi ballatoi e collegamenti ad ogni piano viene ricostituita una ditribuzione ad anello (e non più ad L) che gira intono alla biblioteca e alla corte. L’intervento mira a stabilire una nuova gerarchia tra i nuclei costituenti il complesso edilizio, cui viene aggiunto un nuovo volume con il ruolo di cerniera spaziale, una sorta di piazza interna della facoltà. la vecchia corte infatti viene vetrata in copertura e facciata con una grande struttura in acciaio sorretta da tre pilastri-albero in acciaio: elementi figurativi che giocano sull’ambiguità tra interno-abitato e esterno-giardino, natura e tecnologia. Questa grande stanza vetrata è animata da ballatoi sospesi che collegano i corpi di fabbrica esistenti e la copertura è attrezzata in maniera tale da difendere il grande spazio dall’irraggiamento solare.
Altro tema fondamentale di progetto è stato lo studio di nuovi spazi per lo studio e per il miglioramento del rapporto tra didattica e ricerca. E’ stato incrementato il numero delle aule ricostruendo i volumi che circondano l’aula magna e ogni spazio della didattica è stato attrezzato in modo da garantire isolamento acustico, luminosità, versatilità degli spazi e dotazione tecnologica. E’ stata adoperata una differenziazione tra aule per le lezioni e aule per lo studio individuale.
La biblioteca è stata ampliata e rifunzionalizzata, garantendo un percorso interno più efficiente e rendendola un‘area sudio adatta a permanenze più lunghe e non solo a rapide consultazioni. L’aula magna al suo interno è rimasta invariata ma all’esterno è stata dotata di un rivestimento fotovoltaico che contribuisce all’alimentazione dell’edificio
On the origin of cordierite bearing rhyolites from Mt S. Angelo (Lipari, Italy): fluid inclusions and petrological studies
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