1,720,980 research outputs found
Fecal organic anions in diarrhoeal diseases. Scand. J. Gastroenterol. 1987;22 (suppl.129): 105-109
No full textAbsen
High fibre and colonic fermentation products in man. Ital. J. Gastroenterol. 1988;20:6-10
No full textHuman colonocytes in primary culture: a model to study epithelial growth, metabolism and differentiation. Int. J. Colorect. Dis. 1994;9:13-22
No full textThe purpose of this work was to set up an in vitro model for the study of normal
and pathological functions of the colonic epithelium. We have isolated colonic
crypts by mild proteolytic digestion and mechanical dissociation of human biopsy
material obtained during colonoscopy. The crypts, free of connective tissue, when
placed in culture rapidly attached to the substrate and formed colonies
containing over 95% of epithelial cells. Histochemical and ultrastructural
characterization of the colonies showed the presence of both absorptive and
secretory cells, exhibiting a high degree of differentiation. Proliferative
activity occurred mostly during the first 24 h and progressively declined
thereafter. The cells survived and maintained differentiated characteristics for
at least three days in culture. This method can be used to study normal functions
of the colonic epithelium. It may also be employed to investigate both noxious
and protective factors in pathological conditions such as inflammatory bowel
disease and colorectal neoplasia
Scanning and Transmission Electron Microscopic Observations of Microarterial Anastomosis
No full textSeeking clues for a positive diagnosis of the irritable bowel syndrome. Eur. J. Clin. Invest. 1987;17:189-193
No full textDespite its high prevalence the irritable bowel syndrome (IBS) lacks acceptable
pathophysiological markers and its diagnosis largely depends on the exclusion of
underlying organic disease. Systemic acid-base balance, serum electrolytes and
the composition of faecal water (electrolytes and organic anions), were studied
in thirty-eight diarrhoeal patients out of a series of ninety-three consecutive
IBS patients. Only patients with diarrhoea as the predominant symptom were
included in the study to evaluate whether this subgroup could provide the clue
for a positive diagnosis of the syndrome. Serum electrolytes and systemic
acid-base balance were within the normal range. Faecal electrolytes were also
normal (Na 26.6 +/- 19.3 SD; K 66.8 +/- 28.3; Cl 19.1 +/- 15.2 mEq 1(-1)),
despite the finding of a moderately increased 24-h faecal output. The K:Na ratio
was also within the normal range. These data are in agreement with the lack of
systemic changes observed in IBS patients even with profuse or longstanding
diarrhoea. Both faecal short chain fatty acids and lactic acid were increased in
patients vs. controls, but a considerable overlap with normal values was observed
(131.4 +/- 62.6 SD vs. 108.5 +/- 58.3 mEq 1(-1). Only lactic acid concentration
was significantly higher than in controls (1.3 +/- 1.2 vs. 0.5 +/- 0.2). Despite
these findings it is concluded that the subgroup of IBS patients with diarrhoea
also appears to lack a pathophysiological marker and does not provide clues for a
positive diagnosis of this syndrome
Studio al microscopio elettronico a scansione ed a trasmissione di microanastomosi arteriose
No full text
Microanastomosi Arteriose (diam. 1 mm) studio al microscopio elettronico a scansione ed a trasmissione
No full text- …
