1,721,036 research outputs found
Genetic variability and phylogeny of Torque teno sus virus 1 (TTSuv1) and 2 (TTSuV2) based on complete genomes.
No full textGenetic variability and philogeny of Torque teno sus virus 1 (TTSuV1) and 2 (TTSuV2) based on complete genomes
No full textA novel rolling circle amplification assay to detect members of the family Anelloviridae in pigs and humans
No full textA novel rolling circle amplification assay to detect members of the family Anelloviridae from pigs and humans
No full textRapid rates of evolution in members of the Genera Iotatorquevirus (Torque teno sus virus 1 and 2).
No full textA novel rolling circle amplification assay to detect members of the family Anelloviridae in pigs and humans
No full textThe study describes a novel rolling circle amplification (RCA) assay to detect members of the family Anelloviridae from swine and human serum samples. The RCA was carried out using short anellovirus (AV)-specific primers based on a highly conserved region among available AV full-length genomes. Then, RCA products were used as templates to amplify full-length genomes with an AV-specific PCR. Amplification products were separated by agarose gel electrophoresis and full-length genomes were isolated based on the known size. With this novel AV-RCA/PCR approach it was possible to detect Torque teno sus virus 1 (TTSuV1) and Torque teno sus virus 2 (TTSuV2) in swine and many species of Torque teno virus (TTV) in human sera, which were previously tested negative by conventional PCRs. © 2011 Elsevier B.V
Cyclovirus Vietnam DNA in immunodeficient patients
No full textCyclovirus Vietnam (CyCV-VN) is a CyCV detected in 2013 from cerebrospinal fluid (CSF) samples of patients with neurological disorders. Information on prevalence, pathogenesis and disease association of CyCV-VN is still very patchy
Investigation on torquetenovirus (TTV) microRNA transcriptome in vivo
No full textTorquetenovirus (TTV) is a widespread anellovirus that establishes persistent infections in human showing an increased viremia in immunosuppressed patients. TTV possesses microRNA (miRNA)-coding sequences that might be involved in viral immune evasion. Here, the presence of TTV DNA and miRNAs expression was investigated in plasma samples of 77 diseased (20 infected with human immunodeficiency virus (HIV), 18 infected with hepatitis B (HBV) virus, 18 infected with hepatitis C (HCV) virus, 21 solid organ transplanted) patients, and 25 healthy controls. TTV prevalence was significantly different in healthy controls (60%, 15/25) versus diseased patients (80%, 62/77), showing the highest TTV loads in transplant recipients. Genetic TTV analysis showed the highest prevalence of group 1, followed by groups 3, 4 and 5, and a lack of isolates of group 2. The expression of at least one TTV miRNAs of group 1, 3 and 5 was found in exosomes of plasma of the great majority of individuals (96%, 98/102 subjects) showing the higher prevalence of miRNAs of TTV group 3 (90%, 92/102), followed by miRNAs of group 1 (66%, 67/102), and miRNA of group 5 (49%, 50/102). TTV miRNAs expression and TTV viremia were not always directly correlated, and significant differences appeared in production of some TTV miRNAs between healthy controls and diseased patients. The reported TTV miRNAs status in exosomes encourages further investigation to understand their potential role in the expansion of anelloviruses upon immunosuppression
Torque teno virus viremia correlates with intensity of maintenance immunosuppression in adult orthotopic liver transplant
Full text linkShort-term kinetics of TTV viremia after induction immunosuppression confirm T lymphocytes as the main replication-competent cells
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