4 research outputs found
Promoter sequence analysis of ATP citrate lyase gene in buffalo and Vechur cattle
No full textA study has been carried out to analyze the promoter region of ATP citrate lyase (ACLY) gene in Murrah buffalo (Bubalusbubalis) and native Vechur cattle. PCR amplification of the promoter region generated a fragment of 768 bp in both Buffalo and Vechur cattle.Sequence analysis revealed a few elements/consensus sequences, which have potential role in regulation of transcription. TATA box is found to be absent in the promoter region whereas, TATA like sequence, CAAT box, Cyclic AMP responsive element (CRE), Fat Specific Element (FSE), Enhancer core, Nuclear factor-k Binding site (NF-kB), E- box and inverted CAAT box are present. Comparison of these sequences between ruminants and non-ruminants revealed the presence of important sequence motifs such as, CRE, inverted CAAT boxes and a CAAT box situated far away from the transcription initiation site, together with the absence of a TATA box. All these findings correlate with down regulation of the gene resulting in a low level of expression in ruminants. Phylogenetic analysis of the nucleotide sequences revealed clustering of buffalo, Vechur cattle and Cattle on one clade and Rat and Human delineated from other species and clustered out on a separate clade
Analysis of differential expression of microRNA, bta-miR-93 in lipopolysaccharide challenged peripheral blood mononuclear cells of cattle
No full textThe present study was undertaken to analyse the differences in the microRNA
(miRNA) expression in lipopolysaccharide (LPS) challenged and unchallenged peripheral blood
mononuclear cells (PBMCs) of cattle. The research work was carried out in adult apparently
healthy female crossbred cattle maintained at the University Livestock Farm and Fodder Research
and Development Scheme, Mannuthy. The expression of miRNA and bta-miR-93 was profiled in
the present study by qRT-PCR assay. In silico analysis was done using various online bioinformatic
tools for the prediction of target genes, gene ontology analysis and also to study the cellular
pathways associated to the target genes of bta-miR-93. The expression analysis of bta-miR-
93 showed decrease in its expression in LPS treated PBMCs when compared to control cells.
Besides, significant enrichment of target genes of bta-miR-93 was noticed in many immune-related
GO terms and in critical immune-associated cellular pathways. The findings of the current study
will help in understanding the regulatory role of bta-miR-93 in LPS-mediated immune responses in
cattle
