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Quantitative Trait Loci affecting the somatic cell score on chromosome 4 and 26 in Italian Holstein cattle
Full text linkThis work aimed to confirm previously reported quantitative trait loci (QTL) affecting the somatic cell score (SCS) in dairy cattle on Bos taurus autosomes (BTA) 4 and 26. A granddaughter design with selective genotyping was implemented that included half-sib families from 12 male lines of Italian Holstein cattle. The animals were genotyped for 5 microsatellite markers each on regions of BTA 4 (average marker spacing 9.42 cM) and BTA 26 (average marker spacing 5.26 cM), previously reported by other authors as carrying QTL for somatic cell count. Quantitative trait loci analyses were performed using interval mapping by regressing sire breeding values for SCS onto genotype probabilities at 1-cM intervals along the 2 chromosome regions. Breeding values for SCS were estimated for the whole population using a test-day repeatability animal model. Results were not significant on a chromosome basis, but a possible QTL was found at BM4505 on BTA 26, confirming this region for further studies of QTL affecting SCS in the Italian Holstein population
Tursiops truncatus genetic analysis by DNA markers
No full textA recent law rules the captive breeding of Tursiops truncatus (T.t.) and prevents the introduction of new wild subjects in Italian delphinariums (D.M. n.469,6/12/2001). Microsatellite markers and D-loop mitochondrial polymorphism are widely used for identity/parentage control and analysis of the origin and genetic variability in several animal species. In the present work we characterized genetically the great part of the subjects of Tursiops truncatus ssp kept in captivity in Italy to get information to improve breeding programmes. Genomic and mitochondrial DNA from peripheral blood, blowhole mucosal swabs or necropsy tissues was extracted from 15 animals. Fourteen microsatellite loci informative in T.t. and 558bp of mitochondrial control region (Genbank#AF155161) were amplified by PCR and analyzed by fluorescent automatised methods. Mitochondrial sequences were aligned by Clustal W., genetic distance analysis calculated and a tree created by Phylip package using NEIGHBOR program and UPGMA option. Microsatellite markers allowed to genotype all the subjects and confirmed the parentage relationships. Mitochondrial control region analysis revealed 9 different haplotypes clustering in four different groups (Mediterranean Sea, Tenerife, Caribbean Sea and Mexican Gulf), according to the declared maternal origin of the capture areas. DNA polymorphism analysis clarified some genetic differences between the individuals of Tursiops truncates and suggests indication to orient mating plans and avoid the access of impreeding
Studio del gene NRAMP1 in relazione alla tubercolosi bovina
No full textDespite eradication programs, bovine tuberculosis is still a sanitary and economic problem in Italy. A different and interesting solution to the problem is the genetic approach, aimed to identify genetic susceptibility/resistance to the disease. As a candidate gene for bovine tuberculosis resistance we studied NRAMP1 gene, which encodes a protein thought to play some role in the priming of macrophages for activation, which in turn regulates the multiplication of a variety of intracellular pathogens, like M. bovis. To study NRAMP1 in cattle, we both sequenced part of the gene, and analysed three linked microsatellites. One of these markers, AR028 was found to be polymorphic with 7 alleles and a slight significant effect has been found for immunoglobulin production to M. bovis antigens
Genetica, prevenzione e diagnosi molecolare delle principali malattie ereditarie del gatto
No full textGenetica, prevenzione e diagnosi molecolare delle principali malattie ereditarie del cane e del gatto
No full textHistocompatibility genes and somatic cell count (SCC) in Italian Holstein Friesian
Full text linkGeni di istocompatibilità e conta delle cellule somatiche (SCC) nella Frisona Italiana –
Lo studio ha considerato l’effetto del Complesso Maggiore di Istocompatibilità (MHC) sulla mastite, clinica e subclinica, utilizzando l’Indice Genetico (I.G.) per la conta delle cellule somatiche: SCC (Somatic Cell Count). Su un totale di 302 tori di razza Frisona Italiana, valutati geneticamente per le cellule somatiche, sono stati analizzati il polimorfismo degli antigeni di istocompatibilità di classe I (test di microlinfocitotossicità locus BoLA-A) e di classe II (PCR/RFLP del locus DRB3 esone 2). L’effetto degli antigeni di istocompatibilità sugli indici genetici è stato valutato con un modello di sostituzione genica
Physiochemical polymorphism in MHC class II peptide binding grove molecule and Somatic Cell Count (SCC) in Italian Holstein Friesian bulls
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