23,301 research outputs found
Solitudo sive vitae patrum eremicolarum per antiquissimu[m] Patre[m] D. Hieronymu[m] eorundé primaru[m] olim conscripta [Material gráfico]: iam verò oeneis laminis.
No full textDedicatoria en verso firmada por Jean Turpin, al Cardenal Alejandro Perret - 1. PAVLVS. Tempore... capit.- 2 MARCO. Praebuit... manu./ M. de Vos fig: - 2. ANTONIUS. Progenie... dolos.- 3. HILARION fugiens... suam.- 4. ABRAHAM. A primis... sibi.- 5. MALCHUS. Acta... subit. - 6. IOANNES. Duxit... iugum. - 7. THEONAS. Officio... restitubeat ope. - 8. APOLLONIUS. Solinagus... sacre. - 9. MUTIUS excellens... humo. - 10. HELENUM. Mundities... sibi. - 12. ARSENIUS. Omnibus... fuit / Martín de Vos in. - 12. PAPHNUTIUS. Duxit... polis. - 13. DIDYMO. Tanta... malis. - 14. CALUPPANUS. Haud... pretio. - 14. HELIAS. Pregravis... sequi. - 15. SPIRIDIONIS. Tirones...dolos. - 16. EULOGIUS. Mahuit...suae. - 17. FRIARDUM . Nunneti... Deum / M. de Vos fig: - 17. APELLES. Moribus... manu. - 18. ORIGENES. In tristi... noui. - 19. EVAGRIUS sacri... dabat. - 20. OR deserta... fuit. - 20. EGIDIUM regis... potest / M. de Vos in. - 21. COPRES. Numinis... replet. - 22. MACHARIUS. Mundanum... Deum. - 23. SIMEON. Arua...rapit / Marti de Vos fig: - 23. MACHARIUS. Alter...feras. - 24. ZOERARDE. Haec toti...potest / Martín de Vos figuravit. - 24. ANUB. Magnus... iubet. - 25. CIOMUS. Et si non...fuit. - 26. BRUNO. Carthusiae...timor / M. de Vos figuravit -26. AMMON in Nitra... gregem. - 27. ONOFRIVS. Divinis... esuriem. - 28. PIAMON. In memore... modo. - 29. HIRONYMVS. Multiplici... styl
Sylvae Sacrae [Material gráfico] : Monumenta sa[n]ctioris philosophie quam severa anachoretarum disciplina vitae et religio docuit : Illmo. et Rmo. D.D. Alexandro Perreto Sti. Laurentii in Damaso Diacono Card. de Montealto...
No full text- 1. Iconius CHARITON... preces / M. de Vos invent - 3. EPHREM. Soli... malos / M. de Vos in. - 4. GUIDONE. Itala... queat / M. de Vos fig. - 6. EPIPHANIUS. Isacidum... preces / M. de Vos fi. - 7. IACOBE. Dextera... tumulo / Martí de Vos fig. - 8. BLASIUS hoc... manus / Martí de Vos figur. - 16. MARTINIANUS. Primitias... iter / Martin de Vos figur. - 28. DESIBODE. Dum... tuis / M. de Vos figu
Distinctness effects on VOS order: Evidence from Yucatec Maya
No full textSkopeteas S, Verhoeven E. Distinctness effects on VOS order: Evidence from Yucatec Maya. In: Avelino H, ed. New Perspectives in Mayan Linguistics. Newcastle: Cambridge Scholars Publishing; 2011: 275-300
Distinctness effects on VOS order: Evidence from Yucatec Maya
No full textSkopeteas S, Verhoeven E. Distinctness effects on VOS order: Evidence from Yucatec Maya. MIT Working Papers in Linguistics. 2009;59(New Perspectives in Mayan Linguistics):135-152
First person - Ivo de Vos
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Ivo de Vos is first author on 'The novel zebrafish model pretzel demonstrates a central role for SH3PXD2B in defective collagen remodelling and fibrosis in Frank-Ter Haar syndrome', published in BiO. Ivo conducted the research described in this article while a Research Fellow in Professor Maurice van Steensel's lab at the Skin Research Institute of Singapore (SRIS), Agency for Science, Technology and Research (A*STAR), Singapore. He is now a Postgraduate House Officer in Clinical Genetics, currently working in patient care in the Department of Genetics, at the University Medical Center Groningen (UMCG), The Netherlands, investigating pathophysiological mechanisms underlying common skin conditions by studying rare genetic skin disorders, ultimately improving patient care
Co-occurrence of author’s specific keyword, source: Formulated by author using VOS viewer.
No full textCo-occurrence of author’s specific keyword, source: Formulated by author using VOS viewer.</p
Co-authorship network of authors selected papers, source: Formulated by author using VOS viewer.
No full textCo-authorship network of authors selected papers, source: Formulated by author using VOS viewer.</p
Co-authorship network of authors grouped by affiliated countries, source: Formulated by author using VOS viewer.
No full textCo-authorship network of authors grouped by affiliated countries, source: Formulated by author using VOS viewer.</p
Molecular cloning and expression of full-length DNA copies of the genomic RNAs of cowpea mosaic virus
No full textThe experiments described in this thesis were designed to unravel various aspects of the mechanism of gene expression of cowpea mosaic virus (CPMV). For this purpose full-length DNA copies of both genomic RNAs of CPMV were constructed. Using powerful invitro transcription systems RNA transcripts closely resembling the viral RNAs were prepared from these clones, which were efficiently translated invitro and were able to infect cowpea protoplasts. Mutations were introduced in specific regions in the cDNA clones using methods for site-direced mutagenesis. Investigation of the effect of these mutations on viral gene expression has increased our insight in the multiplication cycle of CPMV.Most of the mutagenized cDNA clones described in this thesis were designed to study the proteolytic processing of the CPMV polyproteins. The proteolytic processing of CPMV lends itself very well to be studied invitro because it reliably mimicks the processing invivo upon translation of the viral RNAs in rabbit reticulocyte lysates (Pelham, 1978; Franssen etal ., 1984). The results obtained here clearly indicate that the B RNA encoded 24K polypeptides is the proteinase involved in all cleavage reactions in the CPMV polyproteins. The question remains however how to processing of the CPMV polyproteins is regulated invivo . Some cleavage reactions which occur slowly invitro occur so rapidly invivo , that the precursor polypeptides cannot be detected. In the proteolytic processing of the M RNA encoded polyproteins. the 32K polypeptide also plays a role. because in its absence the glutamine-methionine site is cleaved very oorly by the 24K proteinase (chapter 6). Furthermore it is not clear whether the 24K polypeptide is proteolytically active in infected cells in the form one or mroe of the precursor polypeptides: the 170K, 110K and 84K polypeptides or as a free protein. It may therefore be expected that the proteoytic cleavages invivo are strictly regulated depending on the different processes in the life cycle of the virus, like RNA multiplication and virus assembly.The only way to gain more insight in the regulation of the proteolytic cleavage reactions is to study the expression of specifically modified cDNA clones invivo . In view of that the expression of RNA transcripts of fulllength cDNA clones in cowpea protoplasts is very promising. The expression of such transcripts in protoplasts was successful, but their infectivity in comparison to viral RNA was rather low and therefore the usefulness of these transcripts at this moment is limited. Besides the infection of whole cowpea plants could not be achieved. It is obvious that the infectivity of the RNA transcripts needs to be improved to increase their applicability. The difference in infectivity between viral RNA and the invitro synthesized RNA sent originate from the different properties of the RNA molecules. Compared to viral RNA the invitro synthesized RNAs lack the genome-linked protein VPg and have two additional nucleotides at the 5' end and 4 to 5 extra nucleotides to the 3' end of the RNA. The invitro synthesized RNA transcripts of full-length cDNA clones of tobacco mosaic virus (TMV) and bromo mosaic virus (BMV) have a relatively high level of infectivity (Damson etal ., 1986; Meshi etal ., 1986; Ahlquist etal ., 1984), but these transcripts have exactly the same 5' end as the viral RNAs; i.e. a cap structure. Using one of the currently known invitro transcription systems it seems difficult to synthesize invitro transcripts from DNA copies of CPMV RNA, which have exactly the same 5' end as the viral RNAs. It may be hoped that the infectivity of the transcripts will increase if the number of extra nucleotides at the 5' end is reduced, but the synthesis of such transcripts will most likely be less efficient (Kang and Nu, 1987; Dunn and Studier, 1983). It in also possible that the extra nucleotides at the 3' end affect the infectivity, although in case of other viruses for which the invitro transcription of cDNA clones has been reported sofar a limited number of extra nucleotides added to the 3' end did not significantly influence infectivity (Ahlquist etal ., 1984: Van der Werf etal ., 1986; Dasmahapatra etal ., 1986; Meshi etal ., 1986; Dawson etal ., 1986). It should be possible to construct cDNA clones, which allow the invitro synthesis of RNA transcripts terminating in a poly(A) tail without extra nucleotides at the 3' end using a restriction enzym for linearization of the template DNA, which restricts the DNA upstream of its recogntion sequence like e.g. BspM1.The possibilities for investigating the various aspects of the multiplication cycle of CPMV and of virus-host interaction will increase significantly when RNA transcripts can be synthesized which are much more infectious than the present ones. Since B RNA replicates independently in cowpea protoplasts because it encodes all proteins necessary for replication of the viral RNAs and proteolytic processing of the polyproteins. various mutations can be introduced in M RNA transcripts without affecting the multiplication and expression of B RNA. The infection of cowpea protoplasts with B RNA transcripts together with specifically modified K RNA transcripts may lead to a better understanding of the proteolytic processing of the M RNA encoded polyproteins invivo and to the elucidation of sequences involved in viral RNA replication.</TT
A vos marques... Prêtes ? Bombez !
No full textM. , Lydie , Moruni . A vos marques... Prêtes ? Bombez !. In: Marie Pas Claire, n°2, 1993. Je suis féministe... et, alors ? p. 14
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