2,064 research outputs found
Adenocarcinoma prostatico in un gatto
No full textLe patologie prostatiche nel gatto sono rare e non conosciamo la loro reale incidenza, data la consolidata pratica veterinaria di effettuare l’orchiectomia in giovane età. Pochi casi di neoplasia prostatica sono stati riportati nel gatto,
per la maggior parte si tratta di adenocarcinomi del corpo della prostata e recentemente è stato descritto anche un
adenocarcinoma della porzione disseminata.
Nel gatto, i carcinomi prostatici si sviluppano in età avanzata e la castrazione non sembra condizionare l’incidenza della patologia
Culture and the Legacy of Anthropology Transatlantic Approaches 1870–1930
No full textThe reader investigates the changing face of the notion of culture, tracing how it emerged in some of the most important and controversial phases of the lively Anglo-American debate on the subject from the late nineteenth century to the mid-twentieth century, including the crucial years of Modernism. Shedding light on the cross-disciplinary approaches that characterized the debate and focusing especially on the legacy of anthropology, the volume presents a selection of some of the most distinguished voices from such assorted fields as literature, linguistics, anthropology, sociology and ethnology, whose interests and areas of enquiry apparently converged and partly overlapped. A selection of primary sources from leading figures such as Matthew Arnold, Bronisław Malinowski, Ruth Benedict, T. S. Eliot, Ezra Pound and Aldous Huxley provide an overview of the crucial issues raised on a wide array of topics: civilization, race, nation, progress, evolution, education, art, science, literature and politics. The primary sources are accompanied by critical essays that offer new insights into these classic texts. This reader will be of use to undergraduate and postgraduate students as well as to scholars exploring the cross-disciplinary or transatlantic nature of the study of culture
Sustainable tourism and development: the model of the Albergo Diffuso
No full textPurpose – In the light of contemporary tourist trends, this paper aims to provide insight on the topicality of
the Albergo Diffuso Model, more than 30 years since this concept was developed.
Design/methodology/approach – The paper is not based on any particular methodological approach,
as it is a practitioner viewpoint paper. It describes both, the contemporary tourist trends and the Albergo
Diffuso Model, its main characteristics and some organizational issues.
Findings – The Albergo Diffuso is still a model worth implementing as it is relevant for the sustainable
development of the Italian territory and of its treasures and to showcase the local traditions and the qualities
of the Italian lifestyle. The Italian Government, too, has acknowledged its potential by means of some recent
initiatives.
Originality/value – This paper highlights the value of the Albergo Diffuso Model as a tool to match the
peculiar features of the Italian territory, local development and the contemporary tourist trends
Effect of lonidamine on alpha2-macroglobulin, hemopexin and alpha1-antitrypsin in the rat testis and epididymis.
No full textIn a recent study (Leone et al., 2000) we reported that lonidamine (LND), an antispermatogenic drug, affected the concentration of selected testicular and epididymal proteins in the rat. Thus, the effect of LND on alpha2-macroglobulin (alpha2-M) and on other two acute phase proteins (APP), hemopexin (HPX) and alpha1-antitrypsin (alpha1-AT) was examined here. LND was administered orally at the dose of 100 mg/kg, the animals were killed after 24 and 48 hr and the samples were analyzed by immunoblotting. The drug did not induce any significant change of alpha2-M in the serum or testis and of HPX and alpha1-AT in the serum, testis or epididymis. Thus, the antispermatogenic action of LND was not accompanied by a significant change of these inflammatory markers, even if it did cause a decrease of alpha1-inhibitor-3, a negative APP, as previously reported (Leone et al., 2000)
Interaction of hyaluronic acid with mucin, evaluated by gel permeation chromatography
No full textHyaluronic acid (HA) is known to increase the ocular bioavailability of ophthalmic drugs not only for its viscous properties but also for its specific affinity for ocular mucins. This phenomenon, called bio- or mucoadhesion, can be evaluated in vitro by mechanical tests which, however, require considerable amounts of mucin (M) that are difficult to obtain from ocular surfaces. Thus, we developed an alternative method, based on gel permeation liquid chromatography, to examine the interaction of HA with microgram quantities of mucin. HA (from human umbilical cord or rooster comb) were fractionated using a Sepharose CL-4B column, before and after incubation with porcine gastric mucin (PGM), and the fractions were analyzed by a specific assay based on the histological dye Stains-all. PGM interacted with high molecular weight (M.W). HA, causing the displacement of low M.W., non-covalently bound, HA fragments, which were eluted under a distinct chromatographic peak. By quantitating the relative area of this peak, an evaluation of the mucoadhesion of HA could be obtained. This method could be useful to study the interaction between HA and microgram quantities of ocular M (mucin), obtained from individual patients or normal subjects
In vitro evaluation of antioxidant activity by electrophoresis and high performance liquid chromatography
No full textTwo methods for the analysis of antioxidants, based on polyacrylamide gel electrophoresis (PAGE) and gel permeation high performance liquid chromatography (HPLC) were developed. Both of them exploit the variations of the signal (band or peak) given by human serum albumin (0.2% w/v in 100 mM sodium phosphate pH 7) upon oxidation with hypochlorite (1% of a solution containing 4% active Cl), quantitatively determined by densitometric analysis or peak integration. Based on such changes, two formulas were defined which allowed the determination of the antioxidant activity of ascorbic acid (EC50,PAGE = 1.5X10(-4) M, EC50,HPLC = 3.6X10(-4) M), glutathione (EC50,PAGE = 1.5X10(-4) M, EC50,HPLC = 2.0X10(-4) M) and melatonin (EC50,PAGE = 5.2X10(-4) M, EC50,HPLC = 3.2X10(-4) M), chosen as reference compounds. A good correlation was found between the activities of these substances in the two assays, which are also in good agreement with literature data, indicating that the two methods are essentially equivalent. These assays could be useful for the screening of new antioxidant drugs for pathological conditions such as cataract, rheumatic diseases, atherosclerosis and Alzheimer's disease. (C) 2000 Elsevier Science B.V. All rights reserved
Quantity discrimination in newly hatched zebrafish suggests hardwired numerical abilities
No full textAn intriguing hypothesis to explain the ubiquity of numerical abilities is that all vertebrates are born with hardwired neuronal networks for processing numbers. To date, only studies on human foetuses have clearly supported this hypothesis. Zebrafish hatch 48–72 h after fertilisation with an embryonic nervous system, providing a unique opportunity for investigating this hypothesis. Here, we demonstrated that zebrafish larvae exposed to vertical bars at birth acquired an attraction for bar stimuli and we developed a numerical discrimination task based on this preference. When tested with a series of discriminations of increasing difficulty (1vs.4, 1vs.3, 1vs.2, and 2vs.4 bars), zebrafish larvae reliably selected the greater numerosity. The preference was significant when stimuli were matched for surface area, luminance, density, and convex hull, thereby suggesting a true capacity to process numerical information. Converging results from two phylogenetically distant species suggests that numerical abilities might be a hallmark feature of vertebrates’ brains
Quantifying groundwater recharge in the Venetian high plain between the Brenta and Piave Rivers through integrated surface–subsurface hydrological modeling
No full textA universal real-time PCR assay for the quantification of group-M HIV-1 proviral load
No full textQuantification of human immunodeficiency virus type-1 (HIV-1) proviral DNA is increasingly used to measure the HIV-1 cellular reservoirs, a helpful marker to evaluate the efficacy of antiretroviral therapeutic regimens in HIV-1-infected individuals. Furthermore, the proviral DNA load represents a specific marker for the early diagnosis of perinatal HIV-1 infection and might be predictive of HIV-1 disease progression independently of plasma HIV-1 RNA levels and CD4(+) T-cell counts. The high degree of genetic variability of HIV-1 poses a serious challenge for the design of a universal quantitative assay capable of detecting all the genetic subtypes within the main (M) HIV-1 group with similar efficiency. Here, we describe a highly sensitive real-time PCR protocol that allows for the correct quantification of virtually all group-M HIV-1 strains with a higher degree of accuracy compared with other methods. The protocol involves three stages, namely DNA extraction/lysis, cellular DNA quantification and HIV-1 proviral load assessment. Owing to the robustness of the PCR design, this assay can be performed on crude cellular extracts, and therefore it may be suitable for the routine analysis of clinical samples even in developing countries. An accurate quantification of the HIV-1 proviral load can be achieved within 1 d from blood withdrawal
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