1,335 research outputs found
Moderate-degree acidosis is an independent determinant of postoperative bleeding in cardiac surgery
No full textBackground. Acidosis is a well-known factor leading to coagulopathy. It has been widely explored as a risk factor for severe bleeding in trauma patients. However, no information with respect to acidosis as a determinant of postoperative bleeding in cardiac surgery patients exists. The aim of this study was to investigate the role of acidosis and hyperlactatemia (HL) in determining postoperative bleeding and need for surgical revision in cardiac surgery patients. Methods. We carried out a retrospective analysis on 4521 patients receiving cardiac operations in two institutions. For each patient the preoperative data and operative profile was available. Arterial blood gas analysis data at the arrival in the intensive care unit were analyzed to investigate the association between acidosis (pH4.0 mMol/L) and postoperative bleeding and surgical revision rate. Results. After correction for the potential confounders, both acidosis (P=0.001) and HL (P=0.001) were significantly associated with the amount of postoperative bleeding. HL was an independent risk factor for postoperative bleeding even in absence of acidosis. Overall, surgical revision rate was 5.6% in patients with HL and no acidosis; 7.7% in patients with acidosis and HL, and 7.2% in patients with acidosis and no HL. All these values are significantly (P=0.001) higher than the ones in patients without acidosis/HL (2%). Conclusions. Even a moderate degree of postoperative acidosis is associated with a greater postoperative bleeding and surgical revision rate in cardiac surgery patients. Correction of acidosis with bicarbonate does not lead to an improvement of the postoperative bleeding asset
Moneta straniera a Pompei in età repubblicana: nuove acquisizioni, in J. UROZ, J. M. NOGUERA, F. COARELLI, (eds.), Iberia e Italia: modelos romanos de integración territorial. IV Congreso Hispano-Italiano, Murcia 26-29 de abril de 2006, Murcia 2008
No full textEffetto dell’integrazione della dieta di pecore con Rosmarinus officinalis sullo stato ossidativo del formaggio Pecorino durante la stagionatura R. BRANCIARI, C. FORTE, M. TRABALZA MARINUCCI, D. RANUCCI, M. ORRU, M. CODINI, R. ORTENZI, D. MIRAGLIA, A. VALIANI
No full textE' stato valutato l ́effetto dell ́integrazione di
rosmarino nella dieta di pecore da latte sulla stabilità ossidativa ed il potere
antiossidante del formaggio Pecorino. La somministrazione di rosmarino
si è dimostrata in grado di influenzare lo stato ossidativo dei formaggi.
I TBARs (Fig. 1) hanno fatto registrare valori inferiori nel gruppo R rispetto
al gruppo CTR a partire da 30 giorni fino alla fine del periodo di
stagionatura. Per quanto riguarda la capacità antiossidante del formaggio
anch’essa è aumentata nel corso della stagionatura ed è risultata
influenzata dal trattamento alimentare fin dal primo giorno
Polyamidoamine grafted cotton with antimicrobial activity
No full textThe widespread of multidrug resistant bacteria, especially in clinical environment, is an important issue. It is necessary to develop new antimicrobial strategies to combine with traditional antibiotics to control the pathogen growth. Cotton fabrics used in a hospital setting can be colonized by pathogens playing an important role in the infection chain of patients, thus the antimicrobial activity of cotton fabrics is a desired add-value. Since linear polyamidoamines (PAAs) showed a certain degree of toxicity against Gram-positive and Gram-negative microorganisms [1], in this study we evaluated the antimicrobial activity of cotton fabrics grafted with antimicrobial PAA chains either directly or through a PEG spacer (Figure 1). The antimicrobial PAA was obtained by polyaddition of N,N’-methylenbisacrylamide (MBA) with N,N’-dimethylethylenediamine (DMEDA). Direct PAA grafting was achieved by first treating cotton with 3 M NaOH for 1 h, then with a 10% (wt/v) MBA aqueous solution for one night at 40 °C. After this time, an equimolar amount of DMEDA compared to MBA was added and the resultant mixture allowed reacting for 72 h. The PEG spacer was introduced by treating MBA-functionalized cotton fabrics with PEG1000-α-ω-bis(1-carbonylpiperazine) previously synthesized [2], then equimolar amounts of DMEDA and MBA were added as in the previous case. The grafted cotton was finally retrieved after exhaustive washing with water and drying.Preliminary antimicrobial tests were performed against the model microorganism Escherichia coli (E. coli) C1a. Samples (1x1 cm) of fabric cotton alone, grafted directly or through PEG spacer with MBA-DMEDA, were inoculated for 2 h with E. coli (106 cfu/fabric). The fabrics were vigorously shaken in nutrient medium and incubated at 37 °C for 24 h. The bacterial growth associated with cotton grafted directly with PAA was as comparable as the control sample. On the other hand, the fabric grafted through PEG spacer inhibited the bacterial growth of E. coli. The positive effect of PEG on the antimicrobial activity displayed by cotton grafted with PAA was confirmed by an “agar patch-test”. Further investigations will be performed to study the mechanism of action.
References
1. E. Caruso, S. Ferrara, P. Ferruti. A. Manfredi, E. Ranucci, V.T. Orlandi, Lasers Med Sci., 2018, DOI: 10.1007/s10103-017-2345-0.
2. E. Ranucci, P. Ferruti, Synthetic Commun. 1990, 20, 2951
Data for: FIBRINOGEN LEVELS COMPENSATION OF THROMBOCYTOPENIA-INDUCED BLEEDING FOLLOWING CARDIAC SURGERY
No full textThrombocytopenic patients with their levels of fibrinogen, bleeding, transfusion
Data for: FIBRINOGEN LEVELS COMPENSATION OF THROMBOCYTOPENIA-INDUCED BLEEDING FOLLOWING CARDIAC SURGERY
No full textThrombocytopenic patients with their levels of fibrinogen, bleeding, transfusion
Le monete, in L. Anniboletti, Progetto Regio VI. Sacello di Vicolo di Narciso (VI 2, 16-21)
No full textAmido-thioether polymers containing pendant carboxylato groups: thermodynamics of protonation and copper(II) complex formation in dilute aqueous solution
No full textCalorimetric data are reported for the enthalpy of protonation of pendant carboxylato groups in new polymers 1a - d containing amido and thioether moieties in their main chain. These data, together with potentiometric and viscometric data, afford a complete thermodynamic description of the protonation process of the new polyacids, which are water-soluble in a narrow range of alpha (degree of protonation) values. Comparison with the non-macromolecular model 2 revealed that the thermodynamic features are attributable to polyelectrolyte effects. In contrast with model 2, all polymers studied form stable hydroxocomplex species with copper(II) ion. The hypothesized [Cu(OH)2L2]2- (where L is the monomeric unit of the polymer) stoichiometry fits the potentiometric data well when processed with the SUPERFIT program. The trend of the corresponding stability constant (log beta) values is to decrease with increasing pH over a wide range
Probing chiral interactions between L- and D-arginine-based polymers and sodium deoxycholate solutions
No full textNowadays the spontaneous self-organization of a polymer into an ordered structure is a soughtafter property of many smart materials, whose applications might range from catalysis [1] to drugdelivery [2]. However, literature regarding the role played by these specific conformations in chiral recognition remains scarce. In this context, polyamidoamino acids (PAACs) are an emerging class of stimuli-responsive bioinspired synthetic polymers able to self-assemble into pH depend conformations [3,4]. PAACs are an off-spring of polyamidoamines (PAAs), a family of polymers obtained by the Michael-type polyaddition of prim-monoamines or sec-diamines with bisacrylamides. The reaction occurs in aqueous solution at pH = 8–9 and at room temperature. By using α-amino acids as monomers, PAACs are obtained.
The first example of PAAC was named ARGO7, obtained by the stepwise polyaddition in water of L- or D-arginine to N,N’-methylenebisacrylamide. Results indicated Mn 8500, PDI 1.4 and Rh of 1.2 nm [3]. Molecular dynamics (MD) and circular dichroism (CD) showed ARGO7 folded into a rigid structure, reminiscent of the hairpin conformation, solely driven by the polymer main chain. Due to its ability to self-assemble in solution forming chiral structures, L- and D-ARGO7 may selectively interact with biological components.
To assess chiral recognition, sodium deoxycholate (NaDC), one of the components of bile salts, was chosen as a chiral model surface. In aqueous solution, NaDC showed three different pH dependent behaviour: homogeneous solution (pH>8), gel phase (pH 7-8) and aggregation/flocculation (pH<6.5). Notwithstanding the ability of NaDC to self-assemble into different conformations at each pH interval, signs of chiral recognition were found in NaDC gel phase only. Conformational modifications were probed by circular dichroism spectroscopy: both D- and L-ARGO7 changed shape and magnitude of the CD pattern, whereas D,L-ARGO7 did not modify the CD spectra of NaDC. After 8 days, NaDC compact structure loosened, ended up being fluid and the CD pattern were completely modified due to NaDC and D- or L-ARGO7 interactions. Incoming diffusion NMR and SANS studies will probably highlight the mechanisms and dynamics of the chiral interactions in these polyelectrolyte-micelle systems.
[1] Luo, R.; Zhu, M.; Shen, X.; Li, S. J. Catal. 2015, 331, 49. [2] Quiñones, J. P.; Peniche, H.; Peniche, C. Polymers. 2018, 10, 3, 235. [3] Manfredi, A.; Mauro, N.; Terenzi, A.; Alongi, J.; Lazzari, F.; Ganazzoli, F.; Raffaini, G.; Ranucci, E.; Ferruti, P. ACS Macro Lett. 2017, 6, 987. [4] Lazzari, F.; Manfredi, A.; Alongi, J.; Mendichi, R.; Ganazzoli, F.; Raffaini, G.; Ferruti, P.; Ranucci, E. Polymers 2018, 10, 1261
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