3,617 research outputs found
Response (letter) to Radiologic evaluation of autoimmune pancreatitis
No full textResponse to a comment on:
Radiology. 2011 Aug;260(2):428-36. Epub 2011 May 25.
Autoimmune pancreatitis: pancreatic and extrapancreatic MR imaging-MR cholangiopancreatography findings at diagnosis, after steroid therapy, and at recurrence.
Manfredi R, Frulloni L, Mantovani W, Bonatti M, Graziani R, Pozzi Mucelli R
A host-vector system for heterologous gene expression in Streptococcus gordonii.
No full textWe have developed a host-vector system for heterologous expression in Streptococcus gordonii (Sg) Challis (formerly Streptococcus sanguis), a commensal bacterium of the human oral cavity. The system is based on (i) integration of plasmid insertion vectors into the chromosome of specially engineered recipient hosts, and (ii) the use of the M6-protein-encoding gene (emm6) as a partner for construction of translational gene fusions. M6 is a streptococcal surface protein already proven useful as a fusion partner for the delivery of foreign antigens to the surface of Sg [Pozzi et al., Infect. Immun. 60 (1992) 1902-1907]. Insertion vectors carry a drug-resistance marker, different portions of emm6 and a multiple cloning site to allow construction of a variety of emm6-based fusions. Upon transformation of a recipient host with an insertion vector, 100% of transformants acquire both the drug-resistance marker and the capacity of displaying the M6 molecule on the cell surface. Chromosomal integration occurred at high frequency in recipient host GP1221. Transformation with 1 microgram of insertion vector DNA yielded 8.1 X 10(5) transformants per ml of competent cells
Correction to: Free Diced Dorsal Augmentation (FDDA) rhinoplasty in non-caucasian patients: tips and tricks (European Journal of Plastic Surgery, (2025), 48, 1, (7), 10.1007/s00238-024-02259-1)
No full textIn this article the author’s name ‘Gianluca Marcaccini’ was incorrectly written as ‘Gianlcua Marcaccini’. Authors ‘Mirco Pozzi’ and ‘Pietro Susini’ should have been denoted as equally contributing author[s]. The original article has been corrected
Su alcuni poeti contemporanei: sei recensioni sul Corriere della Sera
No full textLuzi ha recensito, tra gli altri, sulle pagine del Corriere della Sera alcuni poeti contemporanei: Franca Bacchiega, Elio Fiore, Sebastiano Grasso, Sauro Albisan
Conjugative mobilization of the cloned M6 protein gene from Streptococcus pneumoniae to Streptococcus pyogenes
No full textThe host-vector system omega 6001-pDP36 was used to transfer the M6 protein gene (emm-6.1) of Streptococcus pyogenes to other S. pyogenes strains, isogenic and nonisogenic to D471, the strain from which emm-6.1 was originally cloned. The first step was to subclone emm-6.1 into the insertion vector pDP36. The resulting plasmid, pRMB20, was used as donor in transformation to insert emm-6.1 into the conjugative transposon omega 6001. Streptococcus pneumoniae DP1322, carrying omega 6001 integrated into the chromosome, was the recipient in the transformation experiment. omega 6001 containing emm-6.1 was then transferred by conjugation from S. pneumoniae to the chromosomes of M+ and M- S. pyogenes strains. S. pyogenes transconjugants contained one intact copy of emm-6.1 integrated into the chromosome, but no expression of M6 protein could be detected by Western blot analysis. We found no evidence of the positive transacting regulation of emm gene expression postulated by other authors. In fact, the cloned emm-6.1 was not expressed in three strains expressing their own M proteins (M5, M17 and a shorter M6). In these partial diploids M protein genes were expressed only when present in the original chromosomal locus
The tetracycline resistance gene tet(M) exhibits mosaic structure.
No full textTetracycline resistance genes of the M class, tet(M), are typically found on mobile genetic elements as the conjugative transposons of gram-positive bacteria. By comparing the sequences of eight different tet(M) genes (from Enterococcus faecalis, Streptococcus pneumoniae, Staphylococcus aureus, Ureaplasma urealyticum, and Neisseria), a mosaic structure was detected which could be traced to two distinct alleles. The two alleles displayed a divergence of 8% and a different G/C content. The block structure of these genes provides evidence for the contribution of homologous recombination to the evolution and the heterogeneity of the tet(M) locus. Unlike described cases of chromosomally located mosaic loci, tet(M) is a relatively recently acquired determinant in the species examined and it would appear that mosaic structure within tet(M) has evolved after acquisition of the gene by the mobile genetic elements upon which it is located
Novecento e postmodernità nella critica musicale di Fedele d'Amico. Riflessioni sul metodo storiografico di un corso universitario
No full textThe article examines a university course held in 1978 by the music critic Fedele d’Amico (Rome, 1912 - Rome, 1990) at the Faculty of Letters of the University of Rome La Sapienza. The course offers a reading of the twentieth century, through the analysis of the main movements and composers of the first half of the century, highlighting the historiographical method and the teacher’s critical positions. The historiographical method of Fedele d’Amico attempts to identify the dominant character of the twentieth century through a comparison with nineteenth-century music. This character resides, according to d’Amico, in the general tendency of 20th century composers to be «anti-nineteenth century», to mark the discontinuity with respect to the previous century. In the more radical experiences of modernity represented above all by expressionism, dodecaphony and the serial avant-garde of the Darmstadt mouvement, d’Amico underlines the destruction of the linguistic character of music which, according to him, is the foundation of understanding in musical listening. The author, emphasizing the reductionism of some of d’Amico’s statements, avoids a generic charge of conservatism by relating them to the crisis of modernism and to the spread, in the criticism of the arts during the seventies, of a new postmodern image of the twentieth century
Blast resistance R genes pyramiding in temperate japonica rice
No full textA major issue in rice production is the control of Pyricularia oryzae, the causal agent of rice blast. Genotypes with R resistant leucine-rich repeat genes control specific races of the parasite. However, the resistance is overcome by the pathogen, over a sufficiently long time. In temperate areas, current cultivated varieties have a largely insufficient field resistance. This prompted us to undertake the pyramiding of the R resistance genes Pib, Piz, Pik, Pita2, and Piz-t in temperate japonica materials. Two lines were produced, SJKK and SJKT-2, that have each four pyramided genes. They are fully resistant to rice blast when tested in the field and in greenhouse. However, the tropical origin of the R donor genotypes added complexity to the pyramiding exercise. The results point to a lack of fitness costs in pyramided lines
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