29 research outputs found

    Role of the microbial consortium Enzyveba in the bioremedation of diesel and HiQ diesel-contaminated soils.

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    Diesel fuels spills from pipeline ruptures, underground storage tanks leaking or tanker over turning determine remarkable amounts of diesel fuel hydrocarbons released on areas often dedicated to agricultural activities. As a consequence of their massive production and use as fuel for transportation, they have become one of the most common source of organic pollutants. They generally consist of a complex mixture of linear, branched and cyclic alkanes and aromatic compounds. The biodegradation of such a complex mixture of hydrocarbons usually requires the cooperation of different microorganisms. Such consortia of microorganisms are often absent in the majority of freshly contaminated soils or sites, which do not undergo microbial decontamination promptly. Therefore, the application of unspecified, naturally established complex consortia of microorganisms, such as those occurring in domestic or agro-industry sludge, manure or compost, which generally carry a high diversity of bacteria and fungi along with a variety of essential nutrients, able to sustain survival and colonization of allocthonous microbes in the inoculated biotope, might enhance the bioremediation of diesel-oil mixed contaminated soil. On the other hand, current legislation on the restoration of contaminated sites in some European Countries encourages the employment of some of such sources of microflora, and in particular those obtained from the microbial decomposition/stabilization of “adequate quality organic materials” from municipal wastes, in the biological restoration of contaminated soils and sites (see, as an example, the Italian D. Lgs. No. 152/2006). Enzyveba, a partially characterized complex consortium of not-adapted microorganisms developed through prolonged stabilization of high quality organic wastes produced by Marcopolo Engineering SpA (Cuneo, Italy), was recently found to be able to markedly intensify the aerobic remediation of actual sites aged PAH- and PCB-contaminated soil by acting as a source of both exogenous specialized microorganisms and nutrients. In the present study, the biodegradability of two commercial diesel fuels, i.e., Diesel and HiQ Diesel, spiked to an agricultural soil at 10 g kg-1 was studied under aerobic slurry-phase conditions in the absence and in the presence of Enzyveba, through an integrated chemical-microbiological-ecotoxicological monitoring procedure. A quite similar hydrocarbon composition was displayed by Diesel and HiQ Diesel, which were found to be both extensively and almost completely biodegraded (90%) under all conditions after 4.5 months of treatment. The low initial toxicity, monitored with the animal Folsomia candida mortality test and the plant Lepidium sativum inhibition shoot elongation test, was removed at the end of treatment. The addition of Enzyveba resulted in a higher availability of cultivable specialized bacteria and fungi in the reactors but this only resulted in a slight intensification of soil bioremediation, probably because the pristine soil was rich of microorganism able to degrade diesel fuel. A faster biodegradation of hydrocarbons and a more rapid and extensive depletion of initial ecotoxicity were generally observed in the soil reactors spiked with HiQ Diesel with respect to those spiked with Diesel probably for its content of additives capable of improving hydrocarbons bioavailability

    Enrichment and characterization of Diesel hydrocarbons degrading bacteria from the microbial source Enzyveba

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    The stable consortium of aerobic and anaerobic bacteria and fungi Enzyveba produced by Marcopolo Engineering S.p.A. (Italy) was recently found to significantly intensify the biological remediation of Diesel, PCB and PAH -contaminated soils (1), thus representing a promising inoculum for the bioremediation of polluted soils lacking indigenous active specialized biomass. In this work, Enzyveba’s bacteria able to degrade Diesel hydrocarbons were enriched through five cycles of enrichment cultures in liquid mineral medium with Diesel or Blue Diesel (1000 ppm) as the sole source of carbon and energy. The enriched cultures displayed a similar degradation pathways and potential towards Diesel and Blue Diesel hydrocarbons, which were removed by 62% and 70% after three days of incubation under shaken flask batch aerobic conditions. Accordingly, a remarkable biomass growth (from 106 to 109 CFU/ml) was detected during the incubation. From each culture seven isolates were obtained which are currently under biochemical and molecular characterization. (1) Di Toro S., Zanaroli G., Fava F. Microbial Cell Factories. 2006; 5:11

    Labour markets, poverty and development

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    As we reach the turn of the millennium, the disparity between developed and developing world is of increasing concern. Labour, Poverty, and Development brings together a worldwide mix of contributors from both the academic and practitioner sides of the current debate, combining rigorous economic analysis and broader-based theorizing to provide a detailed picture of the causes, effects, and implications of the current situation in the developing world. All the contributions stress the vertical relationship between macro trends and micro functionings of markets. Part I deals with the interaction between employment and growth and trade policy, investigating the positive relationship betwen growth and emplyment, and the connection between trade liberalization and better working conditions. Part II looks at the situation in urban areas: the crucial and often hidden role that the informal sector plays in the urban employment market and its connection to the formal labour sector, and the collective decision-making involved in gender differentials in education. Finally, Part III investigates the other side of the ruralurban divide, with a detailed micro-study of labour supply in rural communes in China, and an analysis of a common developing-world poverty trap: the spiralling relationship between destitution and low productivit

    PhoNeS: A novel approach to BNCT with conventional radiotherapy accelerators

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    PhoNeS (Photo Neutron Source) is an INFN project devoted to the optimization of the neutron production and moderation in radiotherapy linear accelerators. LinAcs producing high energy (15-25 MeV) photon beams are becoming widespread. At this energy neutron photo-production is unavoidable and the neutron dose must be controlled and reduced during normal radiotherapy. A technique known as BNCT (Boron Neutron Capture Therapy) uses neutrons for radiotherapic treatments: the cells are given a drug containing 10B which undergoes fission after neutron capture, inducing heavy damages to the DNA of the cell itself. This paper will describe the moderator developed by PhoNeS and the results in terms of neutron flux and spectrum and photon contamination of the measurements performed on several radiotherapy accelerators

    PhoNeS: A novel approach to BNCT with conventional radiotherapy accelerators

    No full text
    PhoNeS (Photo Neutron Source) is an INFN project devoted to the optimization of the neutron production and moderation in radiotherapy linear accelerators. LinAcs producing high energy (15-25 MeV) photon beams are becoming widespread. At this energy neutron photo-production is unavoidable and the neutron dose must be controlled and reduced during normal radiotherapy. A technique known as BNCT (Boron Neutron Capture Therapy) uses neutrons for radiotherapic treatments: the cells are given a drug containing B-10 which undergoes fission after neutron capture, inducing heavy damages to the DNA of the cell itself. This paper will describe the moderator developed by PhoNeS and the results in terms of neutron flux and spectrum and photon contamination of the measurements performed on several radiotherapy accelerators
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