88 research outputs found
2-DIMENSIONAL MAPS IN THE MOST EXTENDED (PH 2.5-11) IMMOBILIZED PH GRADIENT INTERVAL
No full textIn conventional isoelectric focusing in soluble buffers, it has been quite difficult to produce two-dimensional (2-D) separation sin pH intervals greater than pH 4-8. In general more alkaline proteins wer analyzed by non-equilibrium IEF in the first dimension. Even with the advent of immobilized pH gradients (IPG), separations could be extended to pH gradients not wider than pH 3-10, due to a lack of suitable buffers. Since more acidic and more alkaline acrylamido buffers have recently been synthesized, we have been able to optimize what is beleived to be the widest possible immobilized pH gradient, a h 2.5-11 span. We report here for the first time 2-D separations of total tissue lysates in such extended pH 2.5-11 gradients. It appears that, with the IPG technique, close to 100% of all possible cell products can be displayed in a single 2-D map
Case Study of Physiotherapeutic care for a patient after anterior cruciate ligament reconstruction
No full textAuthor: Lukáš Praus Thesis Supervisor: Mgr. Jana Sohrová Title: Case Study of Physiotherapeutic Care for a Patient After Anterior Cruciate Ligament Reconstruction Objective: The aim of this thesis is to summarize the issues related to anterior cruciate ligament (ACL) injuries of the knee joint, including the etiology of the injury, treatment methods, and modern physiotherapeutic techniques used in rehabilitation following surgical reconstruction. The thesis also includes a case study of a patient, with the aim of comparing initial and final kinesiological assessments to evaluate the effectiveness of therapy. Methods: The theoretical part was prepared based on academic literature, including recent articles. The practical part contains a case study of a patient, developed during professional practice at the Center for Musculoskeletal Treatment s.r.o. (CLPA) in Prague 9, conducted from January 6, 2025, to January 31, 2025, under the supervision of Jaroslava Tahotná. Results: The objectives of the thesis were fulfilled. The theoretical part provides an overview of the problem, treatment methods, and a comparison of rehabilitation techniques. The case study demonstrates the success of therapy-most short-term goals were achieved, and the patient was educated about self-therapy, which is crucial for...Autor: Lukáš Praus Vedoucí práce: Mgr. Jana Sohrová Název: Kazuistika fyzioterapeutické péče o pacienta po plastice předního zkříženého vazu kolenního kloubu Cíl: Cílem práce je shrnout problematiku poranění předního zkříženého vazu kolenního kloubu, včetně etiologie, terapeutických přístupů a moderních fyzioterapeutických technik používaných při rehabilitaci po chirurgické rekonstrukci. Praktickou částí je kazuistika pacienta, jejímž cílem je porovnat vstupní a výstupní kineziologický rozbor a vyhodnotit efekt terapie. Metody: Teoretická část byla zpracována na základě odborné literatury, včetně aktuálních článků. Speciální část obsahuje kazuistiku pacienta, vypracovanou během odborné praxe v Centru léčby pohybového aparátu s.r.o. (CLPA) na Praze 9 od 6. 1. 2025 do 31. 1. 2025 pod supervizí Jaroslavy Tahotné. Výsledky: Práce splnila stanovené cíle. Teoretická část poskytuje popis problematiky, léčebných metod a porovnání rehabilitačních technik. Kazuistika dokládá úspěšnost terapie - většina krátkodobých cílů byla splněna, pacient byl edukován o autoterapii, která je klíčová pro dosažení dlouhodobých cílů. Závěr: Práce přispěla k rozšíření odborných znalostí o poranění LCA a jeho rehabilitaci. Pozitivní výsledek terapie byl umožněn díky efektivním terapeutickým metodám, odbornému vedení a...Katedra fyzioterapieDepartment of PhysiotherapyFakulta tělesné výchovy a sportuFaculty of Physical Education and Spor
Case Study of Physiotherapeutic care for a patient after anterior cruciate ligament reconstruction
No full textAuthor: Lukáš Praus Thesis Supervisor: Mgr. Jana Sohrová Title: Case Study of Physiotherapeutic Care for a Patient After Anterior Cruciate Ligament Reconstruction Objective: The aim of this thesis is to summarize the issues related to anterior cruciate ligament (ACL) injuries of the knee joint, including the etiology of the injury, treatment methods, and modern physiotherapeutic techniques used in rehabilitation following surgical reconstruction. The thesis also includes a case study of a patient, with the aim of comparing initial and final kinesiological assessments to evaluate the effectiveness of therapy. Methods: The theoretical part was prepared based on academic literature, including recent articles. The practical part contains a case study of a patient, developed during professional practice at the Center for Musculoskeletal Treatment s.r.o. (CLPA) in Prague 9, conducted from January 6, 2025, to January 31, 2025, under the supervision of Jaroslava Tahotná. Results: The objectives of the thesis were fulfilled. The theoretical part provides an overview of the problem, treatment methods, and a comparison of rehabilitation techniques. The case study demonstrates the success of therapy-most short-term goals were achieved, and the patient was educated about self-therapy, which is crucial for..
Laser-induced transient currents in CdZnTe quasi-hemispherical radiation detector
No full textLaser-induced transient currents were measured after applying pulsed or direct-current bias to a CdZnTe quasi-hemispherical radiation detector with gold contacts. The temporal evolution of current transients was analyzed to evaluate the dynamics of the space charge formation and its spatial distribution. The observed effects were explained by a model involving hole injection from positively biased contacts. Experimental results were complemented by numerical simulations, which supported the model. This paper discusses how the detected phenomena affect the detector performance and proposes an improved detector design
Charakterisierung des Glukosestoffwechsels im Schweinherz bei regionaler chronischer Ischämie im Vergleich zum normalen Herzen
No full textThe regional myocardial expression of GLUT1, GLUT3, GLUT4 and hexokinase type II was studied with 2 physiologically differing groups of pigs using real-time PCR and immunoblot: 1-week ischaemia was compared to a control-group with normal perfused hearts. Additionally, perfusion and cellular glucose-uptake was measured using positron-emission-tomographie (PET) to distinguish between vital and non-vital tissue. Results showed a heterogenous regional distribution of the examined proteins and mRNA in normal hearts. In ischaemic segments a distinct shift of mRNA expression from GLUT4 to GLUT1 was detected. This corresponds to an adaption process to reduced oxygen-supply also seen in fetal metabolism.Die regionale myokardiale Expression von GLUT1, GLUT3, GLUT4 und Hexokinase Typ II wurde anhand von 2 physiologisch unterschiedlichen Gruppen von Schweinen mittels real-time PCR und Immunoblot untersucht: 1-wöchige Ischämie wurde einer Kontrollgruppe mit normaldurchblutetem Herzen gegenüber gestellt. Zusätzlich wurde der Blutfluß und die Glukose-Aufnahme der Zellen mittels Positronen-Emissions-Tomographie (PET) erfaßt, um vitales von nicht-vitalem Gewebe zu unterscheiden. Die Ergebnisse zeigten eine heterogene regionale Verteilung der untersuchten Proteine und ihrer mRNA bei Normalherzen. In ischämischen Segmenten konnte eine deutliche Verschiebung der mRNA-Expression von GLUT4 zu GLUT1 nachgewiesen werden. Dies entspricht einem adaptiven Prozess auf reduzierte Sauerstoffversorgung ähnlich einem fetalen Stoffwechselmuster
Secure Control Applications in Smart Homes and Buildings
No full textWith today’s ongoing integration of heterogeneous building automation systems, increased comfort, energy efficiency, improved building management, sustainability as well as advanced applications such as active & assisted living scenarios become possible. These smart homes and buildings are implemented as decentralized systems, where embedded devices are connected via networks to exchange their data. Obviously, the demands – especially regarding security – increase: Secure communication becomes equally important as secure software being executed on the embedded devices. While the former has been addressed by standardization committees, manufacturers and researchers, until now the problem of secure control applications in this domain has not been addressed extensively. This leads to insecure and unprotected software being executed on the embedded devices. Thus, adversaries are capable of attacking building automation systems. This paper introduces an architecture for distributed control applications in smart homes and buildings, which tackles the problem on how to secure software running on different device classes. The following novelties are contributed: an application model capable of depicting control applications in a formal way, the concept of security attributes, being able to formally specify a security policy, and a framework, which allows the secure development and execution of control applications, and an enforcement of the defined security policies
1974 Jay-Cee-An BJC--Page 93
No full textPhotographs of the Lineman's ClubROW1: LEFT TO RIGHT: Tom
Davis, Dan Snell man, Ti m
Weiser, Andy Caylor, Phil Shell,
Randv Sattler, Bob Chubb.
ROW2: LEFT TO RIGHT:
Mike Swanson, Larry
Wittmayer, Cliff Maier, Doug
Dixon, Sid Paulson, Roger Praus
ROW 3: LEFT TO RIGHT:
Terry Kringstad, Terry Hartl,
Jim Hovda, Del Newman.
Dave Short. Tony Wald, Dennis
Baasch ROW 4: LEFT TO
RIGHT: Daryl Engel, Bill
Muller, Rex Baker, Bob
Hendrickson, Bob Scholl, ClydE!
Haack, Sidney Sailer. FORE-GROUND:
LEFT TO RIGHT:
Mi ck Mc Lanahan, instructor;
Slim Rutschke, instructor
9
Secure control applications in smart homes and buildings
No full textDie zunehmende Integration von heterogenen Gebäudeautomationssystemen ermöglicht gesteigerten Komfort, Energieeffizienz, verbessertes Gebäudemanagement, Nachhaltigkeit sowie erweiterte Anwendungsgebiete, wie beispielsweise "Active Assisted Living" Szenarien. Diese Smart Homes und Gebäude sind heutzutage als dezentrale Systeme realisiert, in denen eingebettete Geräte Prozessdaten über ein Netzwerk austauschen. Offensichtlich verändern sich dabei die Anforderungen an derlei Systeme, vor allem hinsichtlich der Informations- und Datensicherheit (Security). Dem Themengebiet sichere Kommunikation kommt dabei ein ähnlich wichtiger Stellenwert zu wie dem Aspekt der Softwaresicherheit. Während erstere Thematik bereits von Standardisierungsgremien und Herstellern aufgegriffen wurde, gibt es bis jetzt keine wissenschaftliche Aufarbeitung, wie das Problem der Softwaresicherheit in diesem Bereich systemweit realisiert werden kann. Kein generisches Angriffsmodell ist bekannt und es fehlt an Sicherheitsempfehlungen. Existierende Schutzmechanismen sind entweder zu zeit- und kostenintensiv oder können nicht einfach auf bestehende Technologien übertragen werden bzw. berücksichtigen nicht die besonderen Anforderungen. Der Entwurf und die Umsetzung von Sicherheitsmaßnahmen wird daher EntwicklerInnen überlassen, die oft aufgrund der Vielfältigkeit des Problems und der Sicherheitsanforderungen überfordert sind. Daraus resultiert, dass Steuerungs- und Regelungsanwendungen unsicher ausgeführt sind, und es Widersachern ermöglicht wird, Gebäudeautomationssysteme anzugreifen. Diese Dissertation stellt eine Architektur für sichere und verteilte Steuerungs- und Regelungsanwendungen in Smart Homes und Gebäuden vor. Damit soll das Problem gelöst werden, wie diese Software sicher auf den unterschiedlichen oft eingebetteten Systemen ausgeführt werden kann. Die folgenden, bisher noch nicht wissenschaftlich aufgearbeiteten Themen, werden diskutiert: eine umfassende Identifikation der Sicherheitsanforderungen, ein Anwendungsmodell, das es ermöglicht Steuerungs- und Regelungsanwendungen formal zu spezifizieren, das Konzept von Sicherheitsattributen, die die Formulierung einer Sicherheitsrichtlinie erlauben und zu guter Letzt, eine Architektur, die die sichere Entwicklung und Ausführung von Steuerungs- und Regelungsanwendungen sowie die Einhaltung von Sicherheitsrichtlinien garantiert.With today's ongoing integration of heterogeneous building automation systems, increased comfort, energy efficiency, improved building management, sustainability as well as advanced applications such as active assisted living scenarios become possible. These smart homes and buildings are implemented as decentralized systems, where embedded devices are connected via networks to exchange their data. Obviously, the demands - especially regarding security - increase: Secure communication becomes equally important as secure software being executed on the embedded devices. While the former has (recently) been addressed by standardization committees and manufacturers, until now no scientific research is available, that targets the problem of secure control applications in this domain. No attack model has been defined, no security measures have been recommended, existing measures from other domains are either too cost or time intensive to deploy, cannot be trivially applied to or do not cover specific demands and constraints of the building automation domain. Thus, deploying adequate control application security measures is left open to developers, who are overburdened with the manifold and often unknown security requirements. This yields to insecure control applications, which enable adversaries to attack building automation systems. This dissertation introduces an architecture for distributed control applications in smart homes and buildings, which tackles the problem on how to secure software running on different device classes. The following novelties are contributed, which - to the best knowledge of the author - have not been addressed in research, yet: a comprehensive identification of security requirements for control applications in smart homes and buildings, an application model capable of depicting control applications in a formal way, the concept of security attributes, being able to formally specify a security policy, and a framework, which allows the secure development and execution of control applications, and an enforcement of the defined security policies
Comparison of graphitic carbon nitrides synthetized from melamine and melamine-cyanurate complex: Characterization and photocatalytic decomposition of ofloxacin and ampicillin
No full textGraphitic carbon nitride (g-C3N4, hereafter abbreviated as CN) was prepared by the heating of melamine (CN-M) and melamine-cyanurate complex (CN-MCA), respectively, in air at 550 °C for 4 h. The specific surface area (SSA) of CN-M and CN-MCA was 12 m2 g−1 and 225 m2g−1 and the content of oxygen was 0.62 wt.% and 1.88 wt.%, respectively. The band gap energy (Eg) of CN-M was 2.64 eV and Eg of CN-MCA was 2.73 eV. The photocatalytic activity of the CN materials was tested by means of the decomposition of antibiotics ofloxacin and ampicillin under LED irradiation of 420 nm. The activity of CN-MCA was higher due to its high SSA, which was determined based on the physisorption of nitrogen. Ofloxacin was decomposed more efficiently than ampicillin in the presence of both photocatalysts
Fast rise of broadly cross-reactive antibodies after boosting long-lived human memory B cells primed by an MF59 adjuvanted prepandemic vaccine
No full textProactive priming before the next pandemic could induce immune memory responses to novel influenza antigens. In an open-label study, we analyzed B cell memory and antibody responses of 54 adults who received 2 7.5-microg doses of MF59-adjuvanted A/Vietnam/1194/2004 clade 1 (H5N1) vaccine. Twenty-four subjects had been previously primed with MF59-adjuvanted or plain clade 0-like A/duck/Singapore/1997 (H5N3) vaccine during 1999-2001. The prevaccination frequency of circulating memory B cells reactive to A/Vietnam/1194/2004 was low in both primed and unprimed individuals. However, at day 21 after boosting, MF59-adjuvanted primed subjects displayed a higher frequency of H5N1-specific memory B cells than plain-primed or unprimed subjects. The immune memory was rapidly mobilized by a single vaccine administration and resulted in high titers of neutralizing antibodies to antigenically diverse clade 0, 1, and 2 H5N1 viruses already at day 7. In general, postvaccination antibody titers were significantly higher in primed subjects than in unprimed subjects. Subjects primed with MF59-adjuvanted vaccine responded significantly better than those primed with plain vaccine, most notably in early induction and duration of cross-reacting antibody responses. After 6 months, high titers of cross-reactive antibody remained detectable among MF59-primed subjects. We conclude that distant priming with clade 0-like H5N3 induces a pool of cross-reactive memory B cells that can be boosted rapidly years afterward by a mismatched MF59-adjuvanted vaccine to generate high titers of cross-reactive neutralizing antibodies rapidly. These results suggest that pre-pandemic vaccination strategies should be considered
- …
