13 research outputs found

    Studies on lipase enzyme production by indigenously isolated Bacillus Cereus (BAIT GCT 127002 ) and Bacillus Cereus (BAIT GCT 127001)

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    Microbial lipases holds a prominent place among biocatalysts that act on carboxylic ester bonds.  This present study involves about eight strains isolated form slaughter house waste water,Coimbatore producing lipase.Among those strains , two bacterial strains exhibiting high lipase production  were identified as  Bacillus Cereus (BAIT GCT 127002 ) and Bacillus Cereus (BAIT GCT 127001 ) by both biochemical analysis and 16S rRNA sequencing.  Initial studies were done for optimizing lipase production using many components such as time course,Carbon sources ,pH and innoculum volume which  revealed maximum lipase acitivity (43.37 U/ml) at 24 hours, using  palm oil , at pH 8 and innoculum volume of 1 ml by Bacillus Cereus (BAIT GCT 127002 ).Further the percentage  removal of oil from oil stained fabric was determined using partially purified lipase with or without detergents and the results indicated 20% ,30% removal with use of  crude lipase and commercial detergent respectively.Hence, lipase from Bacillus Cereus(BAIT GCT 127002 )   can be regarded as an ideal ingredient that can be used in the laundry detergents.Â&nbsp

    Hydroponics agriculture as a modern agriculture technique

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    Purpose Hydroponics, also known as controlled environment agriculture, is a method of cultivating plants and herbs without soil using mineral-supplemented solutions. Agriculture biotechnology enhances its wings on developing genetically modified plants for expanding crop yield and introducing characteristic features such as growing seasonal crops throughout the year, weather-resistant, and pest resistance. Compared to traditional agriculture, hydroponic cultivation yields high-quality crops with 90% more efficient use of water. Therefore, hydroponic cultivation could be considered a succeeding future of agriculture. In this context, understanding agriculture through the hydroponic route is vital for the efficient cultivation of crops. This review elaborates on the different classes of hydroponic systems and the factors that enable the systematic elements of the frame-up. Design/methodology/approach The significant intent of this review is to provide information on distinct hydroponic systems. Findings The present review reports a comprehensive discussion about the significance of the hydroponics system, its mechanism, nutrient solution preparation, types of hydroponic setup, and the challenges faced and would light up the knowledge in the same. Originality/value This review focus on the current feasible hydroponic method of crop cultivation

    Assessment of a Strategy for Valorization of Fatty Acids in the Production of Diazotrophic Microbial Communities with Biofertilizer Potential

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    Ilustraciones, gráficas, tablasLa agricultura actual usa cerca de 100 millones de toneladas anuales de fertilizantes nitrogenados para sostener la demanda alimentaria mundial; la producción de estos fertilizantes y su aplicación en campo ha causado efectos ambientales considerables. Por otra parte, la producción de alimentos también viene asociada con la producción de millones de toneladas anuales de residuos agroindustriales. Se han propuesto alternativas de solución para estas problemáticas basadas en: la teoría ecológica de comunidades microbianas, la ingeniería de bioprocesos y la valorización de residuos agroindustriales con el fin de producir biofertilizantes. En este estudio, nuestro objetivo fue utilizar la bioprospección dirigida centrada en microbiomas, para cultivar comunidades microbianas diazótrofas. Esto se logró mediante el enriquecimiento de muestras de suelo en biorreactores que suministran nitrógeno mediante bombeo de aire y son alimentan con ácidos grasos volátiles como fuente de carbono. Los resultados muestran que a través de un proceso de selección microbiana se puede obtener una comunidad microbiana diazótrofa capaz de fijar 2,7 veces más nitrógeno que el inóculo de partida. Dicha comunidad es capaz de crecer usando ácidos grasos volátiles provenientes de residuos como fuente de carbono. Durante el proceso de cultivo se pueden obtener formas inorgánicas de nitrógeno en el sobrenadante en concentraciones que alcanzan 12,7 mg*ml-1 . La comunidad microbiana diazótrofa crecida en bioreactores, cuyos tres géneros más abundantes son Sinirhodobacter sp. (44,4%), Aureimonas sp. (17,7%) y Taibaiella sp. (12,4%); es capaz de promover el crecimiento de plantas de tomate tanto en hidroponía como en suelo. Alcanzando una altura y producción igual a la obtenida usando fertilizantes nitrogenados de síntesis química con aporte completo de nitrógeno. Se evidencia el potencial de la metodología para hacer crecer una comunidad microbiana diazótrofa con potencial biofertilizante. La sencillez de funcionamiento del reactor y la capacidad de operar con fuentes de carbono provenientes del tratamiento de residuos agroindustriales hace que su aplicación sea prometedora para los países en desarrollo con bajo progreso tecnológico. (Texto tomado de la fuente)Current agriculture utilizes approximately 100 million tons of nitrogen fertilizers annually to sustain global food demand, yet their production and application have led to significant environmental consequences. Additionally, food production generates millions of tons of agro-industrial waste annually. Proposed solutions to these issues involve leveraging ecological theories of microbial communities, bioprocess engineering, and valorization of agro-industrial waste to produce biofertilizers. This study aimed to utilize directed bioprospecting focused on microbiomes to cultivate diazotrophic microbial communities. This was achieved by enriching soil samples in bioreactors that supplied nitrogen through air pumping and were fed with volatile fatty acids as a carbon source. Results demonstrate that through microbial selection, a diazotrophic microbial community capable of fixing 2.7 times more nitrogen than the initial inoculum can be obtained. This community can grow using volatile fatty acids from waste as a carbon source. During cultivation, inorganic forms of nitrogen can be obtained in the supernatant at concentrations reaching 12.7 mg*ml-1 . The diazotrophic microbial community grown in bioreactors, with the three most abundant genera being Sinirhodobacter sp. (44.4%), Aureimonas sp. (17.7%), and Taibaiella sp. (12.4%), is capable of promoting tomato plant growth in both hydroponic and soil environments. Achieving height and production equal to that obtained using chemically synthesized nitrogen fertilizers. The potential of this methodology to cultivate a diazotrophic microbial community with biofertilizer potential is evident. The simplicity of reactor operation and the ability to operate with carbon sources from agro-industrial waste treatment make its application promising for technologically underdeveloped countries.MaestríaMagíster en Ciencias AgrariasSe han propuesto alternativas de solución para estas problemáticas basadas en: la teoría ecológica de comunidades microbianas, la ingeniería de bioprocesos y la valorización de residuos agroindustriales con el fin de producir biofertilizantes. En este estudio, nuestro objetivo fue utilizar la bioprospección dirigida centrada en microbiomas, para cultivar comunidades microbianas diazótrofas. Esto se logró mediante el enriquecimiento de muestras de suelo en biorreactores que suministran nitrógeno mediante bombeo de aire y se alimentan con ácidos grasos volátiles como fuente de carbono.Suelos y AguasCiencias Agropecuarias.Sede Palmir
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