1,720,998 research outputs found
Optimization and validation of a confirmatory method for determination of ten sulfonamides in feeds by LC and UV-diode array detection
No full textA simple, rapid, and reliable multiresidue method, based on liquid chromatography (LC) and ultraviolet (UV)-diode array detection (DAD), is described for assaying ten sulfonamides (sulfadiazine, sulfathiazole, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfamonomethoxine, sulfachloropyridazine, sulfamethoxazole, sulfaquinoxaline, and sulfadimethoxine) in feeds.
The chromatographic separation is accomplished using a C18 column, eluted with a mobile phase consisting of acetate buffer, acetonitrile, and methanol. The sample preparation requires a simple extraction with chloroform/acetone and a purification step by solid-phase extraction. The analytical parameters of precision, detection and quantification limits, recovery, and ruggedness have been evaluated by a validation procedure following the European guidelines of Regulation 882/2004/EC and Decision 657/2002/EC
Assessment of Fumonisins B1 and B2 Levels in Commercial Maize-Based Food Products by Liquid Chromatography with Fluorimetric Detection and Postcolumn Chemical Derivatization
No full textThe occurrence of the fumonisins B1 and B2 in maize-based food products marketed in Italy was examined. A simply and reliable chromatographic method with fluorimetric detection and postcolumn o-phtalaldehyde derivatization was used for a monitoring of 100 samples (8 flours, 21 corn-meal, 16 snacks, 7 maize samples, 13 gluten-free products, and 35 corn-flakes) bought in local supermarkets during the years 2008 and 2009. The presence of both fumonisins B1 and B2, at a concentration higher than 15 μg/kg, was observed in all samples of corn-meal and maize-flour, in 75% of snacks, in 57% of maize samples, in 54% of gluten-free products, and in 29% of corn-flakes. A total of 7 samples including 4 corn-meals, 2 maize-flours, and 1 maize showed a value exceeding the maximum level fixed in the Regulation 1126/2007/EC; no positive sample was observed in corn-flakes, snacks, and gluten-free foods. Fumonisins contamination, on the whole range of maize-based food products analyzed, emphasizes the need of improve agricultural practices, and increase official control and monitoring studies
HPLC, CE ed ELISA: tre tecniche a confronto per la determinazione di istamina nei prodotti ittici
No full textAnalisi di ammine biogene per la valutazione dello stato di freschezza e qualità dei prodotti alimentari
No full textIon-exchange chromatography with conductivity detection for the multiresidual determination of biogenic amines in food products
No full textDetermination of deoxynivalenol and nivalenol by liquid chromatography and fluorimetric detection with on-line chemical post-column derivatization
No full textA rapid, sensitive and selective analytical method was developed for the quantitative determination of deoxynivalenol (DON) and nivalenol (NIV) in cereals intended for human and animal consumption. The method, based on liquid chromatography and fluorescence detection, involves an automated 2 channel post-column derivatization, performed with sodium hydroxide, methyl acetoacetate and ammonium acetate. The chromatographic separation was accomplished using a C18 column eluted in isocratic mode with a mixture of 0.01% acetic acid and acetonitrile. Optimal fluorescence detection was obtained by an excitation and emission wavelength of 360 nm and 470 nm, respectively. The sample preparation required a rapid extraction of mycotoxins with water and a purification step by hydrophilic-lipophilic balance column clean-up. Under the optimized experimental conditions, a complete separation of DON and NIV was obtained in less than 20 min. The on-line post-column derivatization ensures excellent results in terms of simplicity and sensitivity, with limits of detection down to 0.014 mg/kg. The proposed method was extensively validated and the analytical performances of linearity (correlation coefficient of 0.9998), selectivity, precision (intra-day precision lower than 8 %) and recovery (ranging from 89 to 101%) were evaluated, demonstrating the method feasibility in accurate confirmation analyses
Cromatografia a scambio ionico con rivelazione conduttimetrica per un’accurata determinazione di ammine biogene in alimenti a livello di ppb
No full textAflatossina M1 nel latte bovino: determinazione HPLC con rivelazione fluorimetrica e validazione del metodo secondo la decisione 2002/657CE
No full textIon-exchange chromatography with conductivity detection for the multiresidual determination of biogenic amines in food products
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